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  1. No Access

    Article

    Early changes in membrane potential of Saccharomyces cerevisiae induced by varying extracellular K+, Na+ or H+ concentrations

    Recently we introduced a fluorescent probe technique that makes possible to convert changes of equilibrium fluorescence spectra of 3,3’-dipropylthiadicarbocyanine, diS-C3(3), measured in yeast cell suspensions un...

    Jaromír Plášek, Dana Gášková, Jost Ludwig… in Journal of Bioenergetics and Biomembranes (2013)

  2. No Access

    Article

    Monitoring of real changes of plasma membrane potential by diS-C3(3) fluorescence in yeast cell suspensions

    The fluorescent dye 3,3′-dipropylthiadicarbocyanine, diS-C3(3), is a suitable probe to monitor real changes of plasma membrane potential in yeast cells which are too small for direct membrane potential measuremen...

    Jaromír Plášek, Dana Gášková… in Journal of Bioenergetics and Biomembranes (2012)

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    Saccharomyces cerevisiae as Biosensor for Cyto- and Genotoxic Activity

    Conventionally, toxicological bioassays are based on rodent models to evaluate the toxic effects of chemical compounds and to study the mechanism of action of toxicants. However, scientific developments are re...

    Jost Ludwig, Marcel Schmitt… in Atmospheric and Biological Environmental M… (2009)

  4. No Access

    Article

    Electrophysiological and Fluorescence Microscopy Studies with HERG Channel/EGFP Fusion Proteins

    HERG (human ether-a-go-go-related gene) encodes the Kv11.1 protein α-subunit that underlies the rapidly activating delayed rectifier K+ current (I Kr) in the heart. Alterations in the func...

    Sonja Claassen, Sarah Schwarzer, Jost Ludwig… in Journal of Membrane Biology (2008)

  5. No Access

    Article

    Ring test assessment of the mKir2.1 growth based assay in Saccharomyces cerevisiae using parametric models and model-free fits

    Inward rectifying K+ (Kir) channels are a subfamily of the potassium channel superfamily. They mediate potassium influx into the cells, a process responding to the polarization state, a variety of intracellular m...

    Guido Hasenbrink, Lucie Kolacna, Jost Ludwig… in Applied Microbiology and Biotechnology (2007)

  6. No Access

    Article

    Extracellular Mg2+ regulates activation of rat eag potassium channel

    The rat homologue of Drosophila ether à gogo cDNA (rat eag) encodes voltage-activated potassium (K) channels with distinct activation properties. Using the Xenopus expression system, we examined the importance of...

    Heinrich Terlau, Jost Ludwig, Rüdiger Steffan, Olaf Pongs… in Pflügers Archiv (1996)