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  1. Article

    Open Access

    Next-generation fungal identification using target enrichment and Nanopore sequencing

    Rapid and accurate pathogen identification is required for disease management. Compared to sequencing entire genomes, targeted sequencing may be used to direct sequencing resources to genes of interest for mic...

    Pei-Ling Yu, James C. Fulton, Owen H. Hudson, Jose C. Huguet-Tapia in BMC Genomics (2023)

  2. Article

    Open Access

    A synthetic ‘essentialome’ for axenic culturing of ‘Candidatus Liberibacter asiaticus’

    ‘Candidatus Liberibacter asiaticus’ (CLas) is associated with the devastating citrus ‘greening’ disease. All attempts to achieve axenic growth and complete Koch’s postulates with CLas have failed to date, at best...

    Lulu Cai, Mukesh Jain, Alejandra Munoz-Bodnar, Jose C. Huguet-Tapia in BMC Research Notes (2022)

  3. Article

    Open Access

    Broad-spectrum resistance to bacterial blight in rice using genome editing

    Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind s...

    Ricardo Oliva, Chonghui Ji, Genelou Atienza-Grande in Nature Biotechnology (2019)

  4. Article

    Open Access

    Diagnostic kit for rice blight resistance

    Blight-resistant rice lines are the most effective solution for bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo). Key resistance mechanisms involve SWEET genes as susceptibility factors. Bacterial ...

    Joon-Seob Eom, Dang** Luo, Genelou Atienza-Grande, Jungil Yang in Nature Biotechnology (2019)

  5. No Access

    Article

    Penicillin Trunk Injection Affects Bacterial Community Structure in Citrus Trees

    Huanglongbing (HLB), caused by Candidatus Liberibacter asiaticus (CLas), an uncultured α-proteobacterium, is the most destructive disease of citrus trees worldwide. In previous studies, trunk injections of penici...

    Marina S. Ascunce, Keumchul Shin, Jose C. Huguet-Tapia, Ravin Poudel in Microbial Ecology (2019)

  6. Article

    Open Access

    Pacbio sequencing of copper-tolerant Xanthomonas citri reveals presence of a chimeric plasmid structure and provides insights into reassortment and shuffling of transcription activator-like effectors among X. citri strains

    Xanthomonas citri, a causal agent of citrus canker, has been a well-studied model system due to recent availability of whole genome sequences of multiple strains from different geographical regions. Major limitat...

    Alberto M. Gochez, Jose C. Huguet-Tapia, Gerald V. Minsavage in BMC Genomics (2018)

  7. No Access

    Article

    What does it take to be a plant pathogen: genomic insights from Streptomyces species

    Plant pathogenicity is rare in the genus Streptomyces, with only a dozen or so species possessing this trait out of the more than 900 species described. Nevertheless, such species have had a significant impact on...

    Dawn R. D. Bignell, José C. Huguet-Tapia, Madhumita V. Joshi in Antonie van Leeuwenhoek (2010)

  8. No Access

    Article

    Thaxtomin biosynthesis: the path to plant pathogenicity in the genus Streptomyces

    Streptomyces species are best known for their ability to produce a wide array of medically and agriculturally important secondary metabolites. However, there is a growing number of specie...

    Rosemary Loria, Dawn R. D. Bignell, Simon Moll in Antonie van Leeuwenhoek (2008)