29 Result(s)
within
Johannes Messinger
Plant Sciences
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Article
Open AccessOn the simulation and interpretation of substrate-water exchange experiments in photosynthetic water oxidation
Water oxidation by photosystem II (PSII) sustains most life on Earth, but the molecular mechanism of this unique process remains controversial. The ongoing identification of the binding sites and modes of the ...
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Protocol
Measurements of Oxygen Evolution in Photosynthesis
This chapter compares two different techniques for monitoring photosynthetic O2 production; the wide-spread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We...
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Article
Open AccessEvolutionary diversity of proton and water channels on the oxidizing side of photosystem II and their relevance to function
One of the reasons for the high efficiency and selectivity of biological catalysts arise from their ability to control the pathways of substrates and products using protein channels, and by modulating the tran...
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Article
Open AccessSolar energy conversion by photosystem II: principles and structures
Photosynthetic water oxidation by Photosystem II (PSII) is a fascinating process because it sustains life on Earth and serves as a blue print for scalable synthetic catalysts required for renewable energy appl...
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Article
Open AccessThomas John Wydrzynski (8 July 1947–16 March 2018)
With this Tribute, we remember and honor Thomas John (Tom) Wydrzynski. Tom was a highly innovative, independent and committed researcher, who had, early in his career, defined his life-long research goal. He w...
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Article
Open AccessUnequal misses during the flash-induced advancement of photosystem II: effects of the S state and acceptor side cycles
Photosynthetic water oxidation is catalyzed by the oxygen-evolving complex (OEC) in photosystem II (PSII). This process is energetically driven by light-induced charge separation in the reaction center of PSII...
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Protocol
Liquid-Phase Measurements of Photosynthetic Oxygen Evolution
This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We ...
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Article
Gernot Renger (1937–2013): his life, Max-Volmer Laboratory, and photosynthesis research
Gernot Renger (October 23, 1937–January 12, 2013), one of the leading biophysicists in the field of photosynthesis research, studied and worked at the Max-Volmer-Institute (MVI) of the Technische Universität B...
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Article
Warwick Hillier: a tribute
Warwick Hillier (October 18, 1967–January 10, 2014) made seminal contributions to our understanding of photosynthetic water oxidation employing membrane inlet mass spectrometry and FTIR spectroscopy. This arti...
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Article
Efficiency of photosynthetic water oxidation at ambient and depleted levels of inorganic carbon
Over 40 years ago, Joliot et al. (Photochem Photobiol 10:309–329, 1969) designed and employed an elegant and highly sensitive electrochemical technique capable of measuring O2 evolved by photosystem II (PSII) in ...
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Chapter and Conference Paper
An EPR and ENDOR Spectroscopic Investigation of the Ca2+-Depleted Oxygen-Evolving Complex of Photosystem II
Multifrequency EPR and 55Mn ENDOR spectroscopy were used to characterize the S2 ′ state of the Ca2+- depleted oxygen-evolving complex. The 55Mn ENDOR spectrum of the S2 ′ state is ...
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Article
Agu Laisk, Ladislav Nedbal, and Govindjee (eds): Photosynthesis in silico. Understanding complexity from molecules to ecosystems
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Article
Special educational issue on ‘Basics and application of biophysical techniques in photosynthesis and related processes’—Part B
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Article
Open AccessOn-line mass spectrometry: membrane inlet sampling
Significant insights into plant photosynthesis and respiration have been achieved using membrane inlet mass spectrometry (MIMS) for the analysis of stable isotope distribution of gases. The MIMS approach is ba...
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Article
Special educational issue on ‘Basics and application of biophysical techniques in photosynthesis and related processes’
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Article
Open AccessEffects of methanol on the S i -state transitions in photosynthetic water-splitting
From a chemical point of view methanol is one of the closest analogues of water. Consistent with this idea EPR spectroscopy studies have shown that methanol binds at—or at least very close to—the Mn4O ...
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Chapter and Conference Paper
Structure of the Photosynthetic Mn4Ca Cluster Using X-ray Spectroscopy
Single crystals of Photosystem II (PSII) isolated from thermophilic cyanobacteria have been studied by X-ray diffraction (XRD) with resolutions between 3 and 3.8 Å (Ferreira et al. 2004; Loll et al. 2005). The...
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Chapter and Conference Paper
Effects of Chloride/Bromide Substitution on Substrate Water Exchange Rates in Photosystem II
The role of chloride in photosynthetic water oxidation is still under discussion since both its precise function and possible binding site are unresolved. In the present study the effects of Cl− on substrate wate...
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Chapter and Conference Paper
Oxygen Evolving Reactions by Synthetic Manganese Complexes
Four manganese complexes were screened for O2 formation under coherent experimental conditions. We identified several new oxygen evolving reactions and in one case 18O labelling with the expected isotope ratio in...
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Chapter and Conference Paper
Formate-Induced Release of Carbon Dioxide/ Hydrogencarbonate from Photosystem II
Using Membrane-Inlet Mass Spectrometry (Mims) We Confirm That Injections Of High Concentrations (100 Mm Final Concentration) Of Formate Into The Psii Samples Induce A Slow Release Of Carbon Dioxide/Hydrogencar...
