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Article
Heterotrophic nitrification and related functional gene expression characteristics of Alcaligenes faecalis SDU20 with the potential use in swine wastewater treatment
A new heterotrophic nitrifying bacterium was isolated from the compost of swine manure and rice husk and identified as Alcaligenes faecalis SDU20. Strain SDU20 had heterotrophic nitrification potential and could ...
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Article
Open AccessThe role of lung ultrasound B-lines and serum KL-6 in the screening and follow-up of rheumatoid arthritis patients for an identification of interstitial lung disease: review of the literature, proposal for a preliminary algorithm, and clinical application to cases
Screening and follow-up of interstitial lung disease associated with rheumatoid arthritis (RA-ILD) is a challenge in clinical practice. In fact, the majority of RA-ILD patients are asymptomatic and optimal too...
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Article
Construction of recombinant Escherichia coli expressing xylitol-4-dehydrogenase and optimization for enhanced L-xylulose biotransformation from xylitol
L-Xylulose is a rare ketopentose which inhibits α-glucosidase and is an indicator of hepatitis or liver cirrhosis. This pentose is also a precursor of other rare sugars such as L-xylose, L-ribose or L-lyxose. Rec...
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Article
Application of Firefly Luciferase (Luc) as a Reporter Gene for the Chemoautotrophic and Acidophilic Acidithiobacillus spp.
Acidithiobacillus spp. are the most active bacteria in bioleaching and bioremediation, because of their remarkable extreme environmental adaptabilities and unique metabolic characteristics. The researches on regu...
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Article
Optimization for allitol production from d-glucose by using immobilized glucose isomerase and recombinant E. coli expressing d-psicose-3-epimerase, ribitol dehydrogenase and formate dehydrogenase
To develop a method combining enzymatic catalysis and resting-cell biotransformation to produce allitol from low cost substrate d-glucose.
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Article
Ammonium Removal by a Newly Isolated Heterotrophic Nitrification–Aerobic Denitrification Bacteria Pseudomonas Stutzeri SDU10 and Its Potential in Treatment of Piggery Wastewater
A strain SDU10 was isolated from swine manure compost and identified as Pseudomonas stutzeri SDU10. It demonstrated excellent capability in NH4+-N removal. Optimal conditions of NH4+-N removal were determined, wh...
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Article
Efficient Allitol Bioproduction from d-Fructose Catalyzed by Recombinant E. coli Whole Cells, and the Condition Optimization, Product Purification
Allitol is a kind of rare sugar alcohol with potential application value. An engineered strain, which simultaneously expressed d-psicose-3-epimerase (DPE), ribitol dehydrogenase (RDH), and formate dehydrogenase (...
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Article
Production of d-allose from d-fructose using immobilized l-rhamnose isomerase and d-psicose 3-epimerase
d-Allose is a rare sugar, can be used as an ingredient in a range of foods and dietary supplements, has alimentary activities, especially excellent anti-cancer effects and used in assisting cancer chemotherapy .....
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Article
Utilize lung ultrasound B-lines and KL-6 to monitor anti-MDA-5 antibody-positive clinically amyopathic dermatomyositis-associated interstitial lung disease: a case report and literature review
In the past decade, lung ultrasound (LUS) B-lines and serum Krebs von den Lungen-6 (KL-6) antigen have been recognized as biomarkers of the connective tissue disease-associated interstitial lung diseases (CTD-...
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Article
Acute fibrinous and organizing pneumonia as initial presentation of primary Sjögren’s syndrome: a case report and literature review
Acute fibrinous and organizing pneumonia (AFOP) is a new histopathological pattern of acute lung injury first described by Beasley et al. in 2002. Hallmarks of pathological findings are characterized by the pr...
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Article
Modeling and simulation of enzymatic gluconic acid production using immobilized enzyme and CSTR–PFTR circulation reaction system
Production of gluconic acid by using immobilized enzyme and continuous stirred tank reactor-plug flow tubular reactor (CSTR–PFTR) circulation reaction system.
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Chapter
Research of Nonlinear Architecture Morphology Based on Psychology Rules and Mathematical Orders
Technological development is to serve the needs of the human body and mind. Today’s digital technology has become a relatively perfect platform, it has built a new generative architecture form, with numerical ...
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Article
Application of β-glucuronidase (GusA) as an effective reporter for extremely acidophilic Acidithiobacillus ferrooxidans
Acidithiobacillus ferrooxidans is a model organism for investigating metal sulfide bioleaching. The regulatory mechanism of gene expression by metabolizing various substrates is critical ...
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Article
Identification and characterization of an ETHE1-like sulfur dioxygenase in extremely acidophilic Acidithiobacillus spp.
Elemental sulfur (S0) oxidation in Acidithiobacillus spp. is an important process in metal sulfide bioleaching. However, the gene that encodes the sulfur dioxygenase (SDO) for S0 oxidation has remained unclarifie...
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Article
Complete genome of Leptospirillum ferriphilum ML-04 provides insight into its physiology and environmental adaptation
Leptospirillum ferriphilum has been identified as the dominant, moderately thermophilic, bioleaching microorganism in bioleaching processes. It is an acidic and chemolithoautrophic bacterium that gains electrons ...
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Article
Overexpression of Rusticyanin in Acidithiobacillus ferrooxidans ATCC19859 Increased Fe(II) Oxidation Activity
A wide-host-range plasmid pTRUS containing a homologous rus gene under the control of Ptac promoter was constructed and transferred into Acidithiobacillus ferrooxidans ATCC19859 using conjugation gene transfer me...
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Article
Competitive adsorption of binary mixture of Leptospirillum ferriphilum and Acidithiobacillus caldus onto pyrite
Leptospirillum ferriphilum and Acidithiobacillus caldus are two important acidophilic microorganisms involved in iron and sulfur oxidation during bioleaching. Cell adsorption to mineral surfaces is important for ...
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Article
Development and optimization of two-dimensional-electrophoresis protocol ofLeptospirillum ferriphilum
Leptospirillum ferriphilum is important in bioleaching, in which process it is often under heavy stresses of heavy metal ions and high oxidation reduction potential (ORP). Two-dimensional-electrophoresis (2-DE) a...