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Open AccessPredicting hypovitaminosis C with LASSO algorithm in adult critically ill patients in surgical intensive care units: a bi-center prospective cohort study
Vitamin C played pleiotropic roles in critical illness and vitamin C insufficiency was predictive of the development of multiple organ failure. Currently, the prevalence of vitamin C insufficiency in Chinese c...
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Article
The discovery of type IV interferon system revolutionizes interferon family and opens up a new frontier in jawed vertebrate immune defense
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Anti-Aquareovirus Immunity
In this chapter, immunity against aquareoviruses (mainly grass carp reovirus and piscine orthoreovirus) is discussed, including the innate and adaptive immunities. Innate immunity mainly involves the high-mobi...
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Article
The biological features and genetic diversity of novel fish rhabdovirus isolates in China
The Rhabdoviridae is a diverse family of negative-sense single-stranded RNA viruses which infects mammals, birds, reptiles, fish, insects and plants. Herein, we reported the isolation and characterization of 6 no...
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Article
Open AccessThe destiny of the resistance/susceptibility against GCRV is controlled by epigenetic mechanisms in CIK cells
Hemorrhagic disease caused by grass carp reovirus (GCRV) has severely threatened the grass carp (Ctenopharyngodon idella) cultivation industry. It is noteworthy that the resistance against GCRV infection was repo...
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Open AccessLarge-scale SNP screenings identify markers linked with GCRV resistant traits through transcriptomes of individuals and cell lines in Ctenopharyngodon idella
Grass carp (Ctenopharyngodon idella) is an important economic species in freshwater aquaculture and its industry has been confined due to variety degeneration and frequent diseases. Marker-assisted selection is a...
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Article
Open AccessTranscriptome analysis provides insights into the regulatory function of alternative splicing in antiviral immunity in grass carp (Ctenopharyngodon idella)
Characterization of the transcriptomic response to infection is an effective approach to understanding the immune mechanisms. Herein we challenged grass carp (Ctenopharyngodon idella) with grass carp reovirus (GC...
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Article
Dynamic localization and the associated translocation mechanism of HMGBs in response to GCRV challenge in CIK cells
High-mobility group box (HMGB) proteins, a family of chromatin-associated nuclear proteins, play amazingly multifaceted roles in the immune system of mammals. Thus far, little is known about the nucleocytoplas...
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cDNA cloning and characterization of a new member of the tumor necrosis factor receptor family gene from scallop, Chlamys farreri
Tumor necrosis factors receptor (TNFR) is a superfamily of proteins derived mainly from vertebrates. It plays significant role in diverse physiological and pathological events such as inflammation, apoptosis, ...
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Article
Construction and Characterization of Two Bacterial Artificial Chromosome Libraries of Grass Carp
Bacterial artificial chromosome (BAC) library is an important tool in genomic research. We constructed two libraries from the genomic DNA of grass carp (Ctenopharyngodon idellus) as a crucial part of the grass ca...
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Hybrid Cytomegalovirus-U6 Promoter-based Plasmid Vectors Improve Efficiency of RNA Interference in Zebrafish
Short hairpin RNA (shRNA) directed by RNA polymerase III (Pol III) or Pol II promoter was shown to be capable of silencing gene expression, which should permit analyses of gene functions or as a potential ther...
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The Cytomegalovirus Promoter-Driven Short Hairpin RNA Constructs Mediate Effective RNA Interference in Zebrafish In Vivo
The ability to utilize the RNA interference (RNAi) machinery for silencing target-gene expression has created a lot of excitement in the research community. In the present study, we used a cytomegalovirus (CMV) p...
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Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection
Toll-like receptor 3 (TLR3) plays a key role in activating immune responses during viral infection. To study the genes involved in the regulatory function of TLR3 in the rare minnow Gobiocypris rarus after viral ...
