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Article
Somatic embryogenesis of slash pine (Pinus elliottii Engelm.): initiation, maturation, germination and mycorrhization of regenerated plantlets
Slash pine (Pinus elliottii Engelm.), an important economic tree species with a high resin yield, is extensively cultivated in southern China. Somatic embryogenesis (SE) technology could hasten the breeding of P....
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Article
Effects of Plant Growth-Promoting Rhizobacteria on the Growth and Soil Microbial Community of Carya illinoinensis
Plant growth-promoting rhizobacteria (PGPR) are important members of soil microbial communities. In this study, the effects of several PGPR on the growth of Carya illinoinensis plants, the microbial community com...
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Article
Somatic embryogenesis of masson pine (Pinus massoniana): initiation, maturation and genetic stability analysis at SSR loci
Pinus massoniana planted widely in southern China in recent decades. The pinewood nematode (PWN) causes pine wilt disease (PWD), which threatens pine forests, including masson pine. Resistance breeding to pinewoo...
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Article
Open AccessComparative transcriptomic analysis of candidate effectors to explore the infection and survival strategy of Bursaphelenchus xylophilus during different interaction stages with pine trees
The pine wood nematode (PWN), Bursaphelenchus xylophilus, is a devastating pathogen of many Pinus species in China. The aim of this study was to understand the interactive molecular mechanism of PWN and its host ...
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Article
Effects of Different Culture Conditions on the Biofilm Formation of Bacillus pumilus HR10
Many studies have focused on the significant role of biofilm formation by Bacillus in the biocontrol process. Bacillus pumilus HR10 is a plant growth-promoting rhizobacterium with multiple biocontrol functions, i...
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Article
A Bursaphelenchus xylophilus effector, Bx-FAR-1, suppresses plant defense and affects nematode infection of pine trees
Bursaphelenchus xylophilus is an economically important pathogen that has caused serious damage to pine forests. Fatty acid and retinol-binding proteins (FARs) are nematode-specific proteins. One FAR gene, Bx-FAR...
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Article
Isolation and characterization of a mycorrhiza helper bacterium from rhizosphere soils of poplar stands
If mycorrhizal formation could be enhanced by co-inoculation with mycorrhiza helper bacteria (MHB) which promote rapid root colonization by specific ectomycorrhizal fungi, this would be of advantage to the pop...
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Article
Profiling of differentially expressed genes in ectomycorrhizal fungus Pisolithus tinctorius responding to mycorrhiza helper Brevibacillus reuszeri MPt17
A cDNA library of the ectomycorrhizal (ECM) fungus Pisolithus tinctorius Pt2 after interaction with the mycorrhiza helper bacterium (MHB) Brevibacillus reuszeri MPt17 was constructed by suppression subtraction hy...
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Article
Biosafety and colonization of Burkholderia multivorans WS-FJ9 and its growth-promoting effects on poplars
Burkholderia cepacia complex (Bcc) is a group of bacteria with conflicting biological characteristics, which make them simultaneously beneficial and harmful to humans. They have been exploited fo...
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Article
Isolation and identification of phytate-degrading rhizobacteria with activity of improving growth of poplar and Masson pine
A number of soil microorganisms can convert insoluble forms of phosphorus (P) to an accessible form to increase plant yields. Phytate is such a large kind of insoluble organic phosphorus that plants cannot abs...
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Article
NOS-like-mediated nitric oxide is involved in Pinus thunbergii response to the invasion of Bursaphelenchus xylophilus
The content of NO and H2O2 as well as the activities of nitric oxide synthase (NOS)-like and nitrate reductase (NR) were monitored in the needles of Pinus thunbergii infected by Bursaphelenchus xylophilus. The re...
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Article
Effects of ectomycorrhizal fungus Boletus edulis and mycorrhiza helper Bacillus cereus on the growth and nutrient uptake by Pinus thunbergii
The present greenhouse study was undertaken to evaluate the effects of co-inoculating the ectomycorrhizal (ECM) fungus Boletus edulis with the mycorrhiza helper bacterium Bacillus cereus HB12 or HB59 on the growt...
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Article
Isolation and characterization of a new Burkholderia pyrrocinia strain JK-SH007 as a potential biocontrol agent
Poplar canker is a kind of serious disease of poplar branches in China and all over the world. In China, the poplar canker is mainly caused by three pathogens of Cytospora chrysosperma, Phomopsis macrospora and F...
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Article
A nested PCR assay targeting the DNA topoisomerase I gene to detect the pine wood nematode, Bursaphelenchus xylophilus
The pine wood nematode (PWN), Bursaphelenchus xylophilus, is a disastrous pathogen of the pine forests in East Asia and Europe. Plant quarantine is one of the most important ways to prevent its infection in curre...
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Article
Micropropagation of Pinus massoniana and mycorrhiza formation in vitro
A protocol was developed for the micropropagation of Pinus massoniana and mycorrhiza formation on rooted microshoots. Seedling explants were first cultured on Gresshoff and Doy (GD) medium supplemented with 6-ben...
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Article
Detection of the pine wood nematode using a real-time PCR assay to target the DNA topoisomerase I gene
A species-specific real-time PCR assay targeting the DNA topoisomerase I gene has been developed to detect Bursaphelenchus xylophilus. The specificity of the assay was confirmed by the lack of amplification of ge...
