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Article
Stability analysis of new safety cleaning bank in steep slope mining
Based on the study of the slope with gently granular structure in **ngqiao open mine, a new safety cleaning bank mode for steep slope mining was developed, including setting up dint cut, and forming natural re...
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Article
Heat transfer law in leaching dump
Based on the law of temperature changes in the leaching dump and the forming process of heat flux, the basic balance equation of heat flow in dump was established, the dissipated heat flow from dump to the atm...
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Article
Cloning of a Laccase Gene from a Novel Basidiomycete Trametes sp. 420 and Its Heterologous Expression in Pichia pastoris
The laccase gene lacD, cloned from a novel laccase-producing basidiomycete Trametes sp. 420, contained 2,052 base pairs (bp) interrupted by 8 introns. lacD displayed a relatively high homology with laccase genes ...
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Article
Synthesis, crystal structure, electrochemical properties and large optical limiting effect of a novel 3-(E)-ferrocenyl-vinyl-N-hexyl carbazole
The novel compound, 3-(E)-ferrocenyl-vinyl-N-hexylcarbazole (FVHC) was first synthesized and fully characterized by elemental analysis, IR, 1H-NMR, single-crystal X-ray diffraction, ultraviolet (UV) absorption, c...
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Article
Polymorphisms of KIRs Gene and HLA-C Alleles in Patients with Ankylosing Spondylitis: Possible Association with Susceptibility to the Disease
An emerging body of evidence is accumulating to suggest that killer cell immunoglobulin-like receptors (KIRs) and human leukocyte antigen (HLA) class I ligands contribute to the pathogenesis of diverse kinds o...
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Article
Open AccessThe FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species
Plant genome sequencing has resulted in the identification of a large number of uncharacterized genes. To investigate these unknown gene functions, several transient transformation systems have been developed ...
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Article
Production of a cytotoxic cationic antibacterial peptide in Escherichia coli using SUMO fusion partner
Antibacterial peptide CM4 (ABP-CM4) is a small cationic peptide with broad-spectrum activities against bacteria, fungi, and tumor cells, which may possibly be used as an antimicrobial agent. We report here the...
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Article
Open AccessProtocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology
Research in plant molecular biology involves DNA purification on a daily basis. Although different commercial kits enable convenient extraction of high-quality DNA from E. coli cells, PCR and agarose gel samples ...
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Article
Shell-isolated nanoparticle-enhanced Raman spectroscopy
Surface-enhanced Raman scattering (SERS) spectroscopy is a powerful analytical technique able to detect substances down to single molecule level. Its applications are limited, however, because to realize a sub...
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Article
Production of bioactive human hemangiopoietin in Escherichia coli
To devise an efficient approach for production of human hemangiopoietin (hHAPO), the gene of hHAPO was synthesized and subcloned into the pSUMO vector with a SUMO tag at the N-terminus. The expression construc...
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Article
Efficient Expression of Bioactive Human Leptin in Escherichia coli in Soluble Fusion Form
Leptin, a 16 kDa nonglycosylated hormone, is produced by mature adipocytes and functions primarily in the hypothalamus to reduce food intake and body weight. To explore a new approach for high-level expression...
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Article
Production of Bioactive Human Beta-Defensin-4 in Escherichia coli Using SUMO Fusion Partner
The human beta defensins-4 (hBD4) exhibit a broad range of antimicrobial properties and are thought to be ideal therapeutic agents because of their potential ability to circumvent the problems of acquired resi...
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Article
Tool wear criterion, tool life, and surface roughness during high-speed end milling Ti-6Al-4V alloy
The objective of the present research is to investigate the relationship among tool wear, surface topography, and surface roughness when high-speed end milling Ti-6Al-4V alloy, and also to define an optimal fl...
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Article
Polymorphisms of KIR Gene and HLA-C Alleles: Possible Association with Susceptibility to HLA-B27-Positive Patients with Ankylosing Spondylitis
Accumulating evidences indicate that killer cell immunoglobulin-like receptors (KIRs) and their corresponding specific HLA-C ligands contribute to the pathogenesis of multiple autoimmune diseases via the modul...
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Article
Transcriptional regulation by HSV-1 induced HTRP via acetylation system
The protein HTRP (human transcription regulator protein) is encoded by the differential gene htrp and induced by Herpes simplex virus type 1 (HSV-1) infection in KMB-17 cells. HTRP was found to interact with SAP3...
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Article
SUMO Mediating Fusion Expression of Antimicrobial Peptide CM4 from two Joined Genes in Escherichia coli
Antibacterial peptide CM4 (ABP-CM4) is a small cationic peptide with broad-spectrum activities against bacteria, fungi, and tumor cells, which may possibly be used as an antimicrobial agent. To improve the exp...
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Article
SEM study on filum terminale with tethered cord syndrome
To improve the understanding in pathology and provide steady proof for diagnosis and treatment of tethered cord syndrome (TCS), the ultrastructure of filum terminale (FT) with TCS will be researched, respectiv...
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Article
High Efficient Expression of Bioactive Human BMP-14 in E. coli Using SUMO Fusion Partner
Bone morphogenetic proteins (BMPs) are cytokines from the TGF-β superfamily, with important roles during embryonic development and in the induction of bone and cartilage tissue differentiation in the adult bod...
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Protocol
DNA Purification from Multiple Sources in Plant Research with Homemade Silica Resins
DNA purification is a routine procedure in most plant laboratories. Although different kits are available in the market allowing convenient DNA purification, the cumulative cost of purchasing multiple kits for...
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Article
The antibacterial peptide ABP-CM4: the current state of its production and applications
The increasing resistance of bacteria and fungi to currently available antibiotics is a major concern worldwide, leading to enormous efforts to develop new antibiotics with new modes of actions. Antibacterial ...
