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    Protocol

    Protein–Protein Interactions: Co-immunoprecipitation

    Proteins often do not function as single substances but rather as team players in a dynamic network. Growing evidences show that protein–protein interactions are crucial in many biological processes in living ...

    Jer-Sheng Lin, Jemal Ali, Erh-Min Lai in Bacterial Secretion Systems (2024)

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    Protocol

    Protein–Protein Interactions: Yeast Two Hybrid

    The yeast is a powerful and commonly used genetic tool to investigate the interaction between artificial fusion proteins inside the nucleus of yeast. Here, we describe how to use the Matchmaker GAL4-based ...

    Jer-Sheng Lin, Erh-Min Lai in Bacterial Secretion Systems (2024)

  3. No Access

    Protocol

    Protein–Protein Interactions: Co-Immunoprecipitation

    Proteins often do not function as single substances but rather as team players in a dynamic network. Growing evidence shows that protein–protein interactions are crucial in many biological processes in living ...

    Jer-Sheng Lin, Erh-Min Lai in Bacterial Protein Secretion Systems (2017)

  4. No Access

    Protocol

    Protein–Protein Interactions: Yeast Two-Hybrid System

    The yeast two-hybrid system is a powerful and commonly used genetic tool to investigate interactions between artificial fusion proteins inside the nucleus of yeast. Here we describe how to use the Matchmaker G...

    Jer-Sheng Lin, Erh-Min Lai in Bacterial Protein Secretion Systems (2017)