-
Article
Structural and biochemical insight into a modular β-1,4-galactan synthase in plants
Rhamnogalacturonan I (RGI) is a structurally complex pectic polysaccharide with a backbone of alternating rhamnose and galacturonic acid residues substituted with arabinan and galactan side chains. Galactan sy...
-
Article
Open AccessElucidating Human Milk Oligosaccharide biosynthetic genes through network-based multi-omics integration
Human Milk Oligosaccharides (HMOs) are abundant carbohydrates fundamental to infant health and development. Although these oligosaccharides were discovered more than half a century ago, their biosynthesis in t...
-
Article
Open AccessGlycan remodeled erythrocytes facilitate antigenic characterization of recent A/H3N2 influenza viruses
During circulation in humans and natural selection to escape antibody recognition for decades, A/H3N2 influenza viruses emerged with altered receptor specificities. These viruses lost the ability to agglutinat...
-
Article
Measurement of residual dipolar couplings in methyl groups via carbon detection
Residual dipolar couplings (RDCs) provide both structural and dynamical information useful in the characterization of biological macromolecules. While most data come from the interaction of simple pairs of dir...
-
Article
An automated platform for the enzyme-mediated assembly of complex oligosaccharides
An automated platform that can synthesize a wide range of complex carbohydrates will greatly increase their accessibility and should facilitate progress in glycoscience. Here we report a fully automated proces...
-
Article
Streamlining the chemoenzymatic synthesis of complex N-glycans by a stop and go strategy
Contemporary chemoenzymatic approaches can provide highly complex multi-antennary N-linked glycans. These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare adv...
-
Article
A Traveling Wave Ion Mobility Spectrometry (TWIMS) Study of the Robo1-Heparan Sulfate Interaction
Roundabout 1 (Robo1) interacts with its receptor Slit to regulate axon guidance, axon branching, and dendritic development in the nervous system and to regulate morphogenesis and many cell functions in the non...
-
Article
Open AccessPublisher Correction: Network inference from glycoproteomics data reveals new reactions in the IgG glycosylation pathway
Correction to: Nature Communications (2017) 8:1231. doi:10.1038/s41467-017-01525-0
-
Article
Expression system for structural and functional studies of human glycosylation enzymes
A modular protein expression system enables the structural and functional characterization of human glycosyltransferases, glycoside hydrolases and other carbohydrate-modifying enzymes. ...
-
Article
Open AccessNetwork inference from glycoproteomics data reveals new reactions in the IgG glycosylation pathway
Immunoglobulin G (IgG) is a major effector molecule of the human immune response, and aberrations in IgG glycosylation are linked to various diseases. However, the molecular mechanisms underlying protein glyco...
-
Article
Open AccessRestoration of Physiologically Responsive Low-Density Lipoprotein Receptor-Mediated Endocytosis in Genetically Deficient Induced Pluripotent Stem Cells
Acquiring sufficient amounts of high-quality cells remains an impediment to cell-based therapies. Induced pluripotent stem cells (iPSC) may be an unparalleled source, but autologous iPSC likely retain deficien...
-
Article
Open AccessA 72-hour high fat diet increases transcript levels of the neuropeptide galanin in the dorsal hippocampus of the rat
Recent evidence identifies the hippocampus, a brain structure commonly associated with learning and memory, as key to the regulation of food intake and the development and consequences of obesity. Intake of a ...
-
Article
Comparative Gene Expression Signature of Pig, Human and Mouse Induced Pluripotent Stem Cell Lines Reveals Insight into Pig Pluripotency Gene Networks
Reported pig induced pluripotent stem cells (piPSCs) have shown either a bFGF-dependent state with human embryonic stem cell (ESC) and mouse epiblast stem cell (EpiSC) morphology and molecular features or piPS...
-
Article
Effect of xanthohumol and isoxanthohumol on 3T3-L1 cell apoptosis and adipogenesis
Xanthohumol (XN), the chalcone from beer hops has several biological activities. XN has been shown to induce apoptosis in cancer cells and also has been reported to be involved in lipid metabolism. Based on th...
-
Article
Enhancement of ajoene-induced apoptosis by conjugated linoleic acid in 3T3-L1 adipocytes
Ajoene has been shown to induce apoptosis in 3T3-L1 adipocytes. In this report the effects on apoptosis of combinations of ajoene and trans-10, cis-12 conjugated linoleic acid (t10,c12CLA) in 3T3-L1 adipocytes we...
-
Article
Stimulation of melanogenesis by scoparone in B16 melanoma cells
The effect of coumarin derivatives on melanogenesis was investigated in B16 murine melanoma cells.
-
Article
Esculetin induces mitochondria-mediated apoptosis in 3T3-L1 adipocytes
Adipose tissue mass is determined by the volume and the number of adipocytes and is subjected to homeostatic regulation involving cell death mechanisms. We investigated the effects of esculetin, a coumarin com...
-
Article
Open AccessHerba houttuyniae extract induces apoptotic death of human promyelocytic leukemia cells via caspase activation accompanied by dissipation of mitochondrial membrane potential and cytochrome c release
Herba houttuyniae has been used as a constituent of herval medicine prescriptions for the treatment of inflammation, cancer, and other diseases. In the present study, we investigated the cellular effects of herba...
-
Article
Open AccessRetinoic acid inhibits inducible nitric oxide synthase expression in 3T3-L1 adipocytes
The present study was undertaken to explore whether retinoids, which are known to have immunomodulatory actions, could attenuate tumor necrosis factor-α (TNF)-stimulated inducible nitric oxide synthase (iNOS) ...
-
Article
Open AccessStimulation of melanogenesis by glycyrrhizin in B16 melanoma cells
Glycyrrhizin (GR), triterpenoid saponin composed of one glycyrrhetinic acid (GA) and two glucuronic acids, is a main constituent of the hydrophilic fraction of licorice (Glycyrrhiza glabra) extracts and is kno...
