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Article
Open AccessColon cancer cells adopt an invasive phenotype without mesenchymal transition in 3-D but not 2-D culture upon combined stimulation with EGF and crypt growth factors
The intestinal crypt homeostasis is maintained by a combination of growth factors including Wnt, R-Spondin1, Noggin and the epidermal growth factor (EGF). In human colorectal cancer, the Wnt pathway is constit...
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Open AccessErratum to: Exploiting the promiscuity of imatinib
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Open AccessEffect of hydroxyurea on the promoter occupancy profiles of tumor suppressor p53 and p73
The p53 tumor suppressor and its related protein, p73, share a homologous DNA binding domain, and mouse genetics studies have suggested that they have overlap** as well as distinct biological functions. Both...
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Article
Exploiting the promiscuity of imatinib
The protein kinase inhibitor imatinib, also known as Gleevec, has been a notable success in treating chronic myelogenous leukemia. A recent paper in BMC Structural Biology reports a 1.75 Å crystal structure of im...
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Article
Open AccessSystems analysis of quantitative shRNA-library screens identifies regulators of cell adhesion
High throughput screens with RNA interference technology enable loss-of-function analyses of gene activities in mammalian cells. While the construction of genome-scale shRNA libraries has been successful, resu...
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Article
DNA damage-induced cell death: lessons from the central nervous system
DNA damage can, but does not always, induce cell death. While several pathways linking DNA damage signals to mitochondria-dependent and -independent death machineries have been elucidated, the connectivity of ...
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Article
Modulation of human checkpoint kinase Chk1 by the regulatory β-subunit of protein kinase CK2
Protein kinase CK2 is a serine/threonine protein kinase involved in various aspects of cellular regulation. The regulatory β-subunit of CK2 exerts a central role not only in mediating formation of tetrameric CK2 ...
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Article
Regulation of cell death by the Abl tyrosine kinase
The c-abl proto-oncogene encodes a protein tyrosine kinase that is distributed in the nucleus and the cytoplasm of proliferating cells. In the nucleus, c-Abl activity is negatively regulated by the retinoblastoma...
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Article
The SH2-containing adapter protein GRB10 interacts with BCR-ABL
Bcr-Abl is an oncogenic tyrosine kinase expressed in tumor cells of CML and a subset of ALL which in its unregulated and activated state is thought to cause cell transformation and leukemia. Bcr-Abl contains s...
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Article
Hyperphosphorylated p107 and p130 bind to T-antigen: identification of a critical regulatory sequence present in RB but not in p107/p130
The retinoblastoma tumor suppressor RB and its related proteins, p107 and p130, are targets of several viral oncoproteins, including SV40 large T-antigen (T-Ag) and adenovirus E1A. T-Ag and E1A each contains a...
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Article
Effect of Bcr sequences on the cellular function of the Bcr-Abl oncoprotein
In Philadelphia chromosome (Ph1)-positive human leukemia, the c-Abl tyrosine kinase is activated by fusion to sequences encoded by the breakpoint cluster region (bcr) gene. Two major types of Bcr-Abl fusion prote...
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Article
Functional analysis of wild-type and malignant glioma derived CDKN2Aβ alleles: Evidence for an RB-independent growth suppressive pathway
The tumor suppressor gene CDKN2A (p16/MTS1/INK4A), which encodes the cyclin-dependent kinase inhibitor p16INK4a, is a target of 9p21 deletions during the malignant progression of human gliomas. This gene also enc...
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Article
Point mutations can inactivate in vitro and in vivo activities of p16INK4a/CDKN2A in human glioma
Deletions of chromosomal region 9p21 are among the most common genetic alterations observed during the clonal evolution of high grade malignant gliomas. Structural and functional evidence has suggested that ho...