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Chapter
Gene Editing of Wheat to Reduce Coeliac Disease Epitopes in Gluten
By using gene editing technologies such as CRISPR/Cas, precise modifications can be made in the genome. CRISPR/Cas is especially valuable for targeted mutagenesis in polyploids, as it can induce mutations of m...
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Article
Open AccessThe biological feasibility and social context of gene-edited, caffeine-free coffee
Coffee, especially the species Coffea arabica and Coffea canephora, is one of the world’s most consumed beverages. The consumer demand for caffeine-free coffee is currently being met through chemical decaffeinati...
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Article
Open AccessGenome editing of polyploid crops: prospects, achievements and bottlenecks
Plant breeding aims to develop improved crop varieties. Many crops have a polyploid and often highly heterozygous genome, which may make breeding of polyploid crops a real challenge. The efficiency of traditio...
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Article
Open AccessOutlook for coeliac disease patients: towards bread wheat with hypoimmunogenic gluten by gene editing of α- and γ-gliadin gene families
Wheat grains contain gluten proteins, which harbour immunogenic epitopes that trigger Coeliac disease in 1–2% of the human population. Wheat varieties or accessions containing only safe gluten have not been id...
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Article
Open AccessRe-sequencing transgenic plants revealed rearrangements at T-DNA inserts, and integration of a short T-DNA fragment, but no increase of small mutations elsewhere
Transformation resulted in deletions and translocations at T-DNA inserts, but not in genome-wide small mutations. A tiny T-DNA splinter was detected that probably would remain undetected by conventional techni...
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Article
Open AccessErratum: Screening for recombinants of Crambe abyssinica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration
The T-DNA region of pMF1 vector of marker-free system developed by Wageningen UR, has Recombinase R-LBD gene fusion and nptII and codA gene fusion between two recombination sites. After transformation applying de...
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Article
Open AccessScreening for recombinants of Crambe abyssinica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration
The T-DNA region of pMF1 vector of marker-free system developed by Wageningen UR, has Recombinase R-LBD gene fusion and nptII and codA gene fusion between two recombination sites. After transformation applying de...
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Article
Fine map** of the gene Rvi18 (V25) for broad-spectrum resistance to apple scab, and development of a linked SSR marker suitable for marker-assisted breeding
Apple scab, caused by the fungal pathogen Venturia inaequalis, is one of the most devastating diseases for the apple growing industry in temperate zones with humid springs and summers. Breeding programs around th...
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Article
Open AccessRegeneration and transformation of Crambe abyssinica
Crambe abyssinica (crambe) is a non-food oil seed crop. Its seed oil is widely used in the chemical industry because of the high erucic acid content. Furthermore, it is a potential platform for various feedstock ...
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Article
Cloning and functional characterization of the Rvi15 (Vr2) gene for apple scab resistance
Apple scab, caused by Venturia inaequalis, is a serious disease of apple. Previously, the scab resistance Rvi15 (Vr2) from the accession GMAL 2473 was genetically mapped, and three candidate resistance genes were...
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Article
Differences in acidity of apples are probably mainly caused by a malic acid transporter gene on LG16
Acidity has profound effects on the taste of apples (Malus × domestica). Malic acid is the predominant organic acid in apples. Differences in malic acid content are caused by differences in accumulation of malic ...
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Article
Open AccessThe mQTL hotspot on linkage group 16 for phenolic compounds in apple fruits is probably the result of a leucoanthocyanidin reductase gene at that locus
Our previous study on ripe apples from a progeny of a cross between the apple cultivars ‘Prima’ and ‘Fiesta’ showed a hotspot of mQTLs for phenolic compounds at the top of LG16, both in peel and in flesh tissu...
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Article
Open AccessPerformance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines
Introduction of sustainable scab resistance in elite apple cultivars is of high importance for apple cultivation when aiming at reducing the use of chemical crop protectants. Genetic modification (GM) allows t...
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Article
Open AccessFunctional analysis and expression profiling of HcrVf1 and HcrVf2 for development of scab resistant cisgenic and intragenic apples
Apple scab resistance genes, HcrVf1 and HcrVf2, were isolated including their native promoter, coding and terminator sequences. Two fragment lengths (short and long) of the native gene promoters and the strong ap...
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Article
Open AccessIsolation and characterization of strong gene regulatory sequences from apple, Malus × domestica
For the strong expression of genes in plant tissue, the availability of specific gene regulatory sequences is desired. We cloned promoter and terminator sequences of an apple (Malus x domestica) ribulose biphosph...
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Chapter
Functional Molecular Biology Research in Fragaria
The most economically important strawberry species is the octoploid Fragaria × ananassa, grown worldwide over a wide range of latitudes for fresh market and processing industry. The small-fruited diploid F. vesca
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Article
Chromosome elimination and mutation in tetraploid somatic hybrids ofSolanum tuberosum andSolanum phureja
The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetra...
