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Article
Open AccessHyaluronic acid impacts hematological endpoints and spleen histological features in African catfish (Clarias gariepinus)
Since its identification in the vitreous humour of the eye and laboratory biosynthesis, hyaluronic acid (HA) has been a vital component in several pharmaceutical, nutritional, medicinal, and cosmetic uses. How...
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Article
Biodegradable microplastics interaction with pollutants and their potential toxicity for aquatic biota: a review
The global plastic production has steadily increased from 1.7 million tons in 1950 to over 400 million tons in 2022, with about 60% of plastic ultimately ending up in landfills and oceans. There is also growin...
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Article
Acute and multigenerational toxicity of polylactic acid microplastics on a copepod bioindicator
Bioplastics such as polylactic acid are actually promoted as eco-friendly alternatives to fossil fuel-derived plastics, yet bioplastic toxicity remains poorly known. Here we studied the acute and multigenerati...
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Article
Open AccessPolystyrene nanoplastic and engine oil synergistically intensify toxicity in Nile tilapia, Oreochromis niloticus
Polystyrene nanoplastic (PS-NPs) and Engine oil (EO) pose multiple ecotoxic effects with increasing threat to fish ecosystems. The current study investigated the toxicity of 15 days exposure to PS-NPs and / or...
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Article
Open Access3D bioprinted multilayered cerebrovascular conduits to study cancer extravasation mechanism related with vascular geometry
Cerebral vessels are composed of highly complex structures that facilitate blood perfusion necessary for meeting the high energy demands of the brain. Their geometrical complexities alter the biophysical behav...
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Article
Exposure protocol for ecotoxicity testing of microplastics and nanoplastics
Despite the increasing concern about the harmful effects of micro- and nanoplastics (MNPs), there are no harmonized guidelines or protocols yet available for MNP ecotoxicity testing. Current ecotoxicity studie...
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Article
Open Access3D biofabrication of diseased human skin models in vitro
Human skin is an organ located in the outermost part of the body; thus, it frequently exhibits visible signs of physiological health. Ethical concerns and genetic differences in conventional animal studies hav...
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Article
Open AccessAchieving volatile potassium promoted ammonia synthesis via mechanochemistry
Potassium oxide (K2O) is used as a promotor in industrial ammonia synthesis, although metallic potassium (K) is better in theory. The reason K2O is used is because metallic K, which volatilizes around 400 °C, sep...
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Article
Limitations of the Plasmid-Based Cas9-Zinc Finger Fusion System for Homology-Directed Knock-In in Chinese Hamster Ovary Cells
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been used for the insertion of large transgenes into Chinese hamster ovary cells via co-trans...
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Article
Open AccessProtective efficacy of dietary natural antioxidants on microplastic particles-induced histopathological lesions in African catfish (Clarias gariepinus)
Microplastic particles (MPs) are a common environmental pollutant easily ingested by fish in aquaculture. The current study evaluated the protective efficacies of some antioxidant, e.g., lycopene, citric acid,...
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Article
Open AccessEpigenetic plasticity enables copepods to cope with ocean acidification
Plasticity enhances species fitness and survival under climate change. Ocean acidification poses a potential threat to copepods, a major zooplankton group that serves as a key link between the lower and higher...
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Article
The Genome of the Marine Rotifer Brachionus manjavacas: Genome-Wide Identification of 310 G Protein-Coupled Receptor (GPCR) Genes
The marine rotifer Brachionus manjavacas is widely used in ecological, ecotoxicological, and ecophysiological studies. The reference genome of B. manjavacas is a good starting point to uncover the potential molec...
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Article
Effects of zinc and mercury on ROS-mediated oxidative stress-induced physiological impairments and antioxidant responses in the microalga Chlorella vulgaris
The rapid growth of industrialization and urbanization results in deterioration of freshwater systems around the world, rescinding the ecological balance. Among many factors that lead to adverse effects in aqu...
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Next-Generation Cell Engineering Platform for Improving Recombinant Protein Production in Mammalian Cells
Industrial manufacturing of high-value biopharmaceuticals is dominated by mammalian cells. Advances in mammalian cell engineering have paved the way for remarkable achievements in efficient mammalian cell expr...
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Article
CHO Cell Line Development and Engineering via Site-specific Integration: Challenges and Opportunities
Chinese hamster ovary (CHO) cells are utilized as primary mammalian expression host cells for the production of biopharmaceuticals. Recombinant CHO cell line development (CLD) has been a crucial step for thera...
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Article
Benthic Respiration and Nutrient Release Associated with Net Cage Fish and Longline Oyster Aquaculture in the Geoje-Tongyeong Coastal Waters in Korea
Sediment oxygen demand (SOD) and benthic nutrient fluxes were measured at five sites using an in situ benthic chamber: fish farm (FF), oyster farm (OF), Tongyeong Marine Science Station (TMSS), and two control...
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Article
Zinc Pyrithione (ZnPT) as an Antifouling Biocide in the Marine Environment—a Literature Review of Its Toxicity, Environmental Fates, and Analytical Methods
Since the ban of tributyltin in antifouling paints, many alternative biocides have been introduced to prevent settlement and growth of marine organisms on ship hulls. Zinc pyrithione (ZnPT) is one of the most ...
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Article
Targeted cytochrome P450 3045C1 (CYP3045C1) gene mutation via CRISPR-Cas9 ribonucleoproteins in the marine rotifer Brachionus koreanus
The CRISPR-Cas9 system has revolutionized genetic engineering and has been applied in numerous model organisms to date. To examine the capacity of the CRISPR-Cas9 system for generation of mutants in the marine...
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Article
Bioaccumulation of Mercury in the Copepod Pseudodiaptomus marinus: A Comparative Study Between Waterborne and Dietary Pathways
The fate and the accumulation kinetics of mercurychloride (HgCl2) were investigated in the invasive copepod species Pseudodiaptomus marinus, which originates from the North-Western Pacific Ocean and was recently ...
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Article
Genome-wide identification and expression of eight fatty acid desaturase genes, and the fatty acid profile, in the marine rotifer Brachionus koreanus fed the alga Tetraselmis suecica
Fatty acid desaturases catalyze the formation of a C=C double bond from a C–C single bond in fatty acids, leading to diversification of the fatty acid pool. In this study, we identified the fatty acid desatura...
