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  1. No Access

    Chapter

    Modulation of CD4 Expression on Helper T Lymphocytes and U937 Cells by Ganglioside GM3 and Its Derivatives

    Ganglioside GM3 and a variety of chemically modified derivatives (e.g. lyso-GM3, de-N- acetyl-GM3, GM3-amides) have been assayed for their potential upon CD4 modulation on human peripheral blood lymphocytes an...

    D. Heitmann, P. Budde, J. Frey, J. Lehmann… in New Developments and New Applications in A… (2002)

  2. No Access

    Article

    Monitoring of ascorbate at a constant rate in cell culture: Effect on cell growth

    Ascorbic acid (vitamin C) is a primary antioxidant for cells. But, ascorbic acid added to culture medium is not readily available to cells in culture, because it is unstable in aqueous media. We determined the...

    T. Chepda, M. Cadau, Ph. Girin, J. Frey… in In Vitro Cellular & Developmental Biology … (2001)

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    Article

    Mineralization and Alkaline Phosphatase Activity in Collagen Lattices Populated by Human Osteoblasts

    Adult human osteoblastic cells were grown in a native type I collagen gel. Proliferation and viability analyses showed that cells rapidly stopped dividing and became blocked in the G0G1 phase (91% on day 13)....

    A. Rattner, O. Sabido, J. Le, L. Vico, C. Massoubre… in Calcified Tissue International (2000)

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    Article

    Characterization of human osteoblastic cells: Influence of the culture conditions

    Human osteoblastic cells were isolated enzymatically from adult human spongy bone and grown in MEM-Ham F12 1:1 medium supplemented with 2% Ultroser (USM). They were subcultured and examined for osteoblast feat...

    A. Rattner, O. Sabido, C. Massoubre… in In Vitro Cellular & Developmental Biology … (1997)

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    Article

    A direct fluorimetric DNA assay on cell suspensions

    An improved direct fluorimetric assay is described using bisbenzimidazol fluorescence at 356 nm excitation and 458 nm emission wavelengths. The turbidity of the medium was shown to have no effect on fluorescen...

    M. P. Bonis, N. Germain, J. Frey in Journal of tissue culture methods (1991)

  6. No Access

    Article

    Ω mutagenesis in gram-negative bacteria: A selectable DNA fragment which terminates transcription in a wide range of bacterial species

    J. Frey, J. Deshusses, H. M. Krisch in Experientia (1986)

  7. No Access

    Chapter

    Mutagenesis

    Bacterial plasmids are autonomously replicating mini chromosomes (replicons) which contain genetic information for many different properties, including the production of toxins and adhesion antigens, resistanc...

    R. Eichenlaub, A. Pühler, V. Krishnapillai, H. Heilmann… in Advanced Molecular Genetics (1984)

  8. No Access

    Chapter

    Basic Methods

    Gene transfer in bacteria occurs by three main processes: transformation and transfection, which involve the direct uptake of free DNA by bacteria; transduction, which involves bacteriophage mediated transmiss...

    M. P. O’Connell, U. Priefer, N. Datta, M. E. Nugent… in Advanced Molecular Genetics (1984)

  9. No Access

    Chapter

    Gene Cloning

    Genetic recombination is the fundamental biological process of exchange of genetic information between different chromosomes. Its constant activity during evolution has enabled the large number of spontaneousl...

    K. N. Timmis, G. Brady, J. Frey, A. Pühler, U. Priefer… in Advanced Molecular Genetics (1984)

  10. No Access

    Article

    Place de la prolyl hydroxylase dans la synthèse du collagène de cellules KB

    KB cells, which synthetized collagen at a low rate, shown a prolyl hydroxylase activity at the same rate that fibroblast. The relationship between collagen synthesis and prolyl hydroxylase activity in these ce...

    J. Farjanel, P. Vallier, M. C. Ronzière, J. Frey in Experientia (1979)

  11. Article

    Étude de l'activité de la protocollagène proline hydroxylase de foie d'embryon de Poulet en fonction de l'âge

    In chick embryo liver, there was a peak activity of protocollagen proline hydroxylase (PPH) on the 18th day of the embryonnic state. The effect of triton X100 suggested that PPH was a particulate enzyme, in co...

    Geneviève Helfre, J. Farjanel, Juliette Fonvielle, J. Frey in Experientia (1974)

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  12. No Access

    Article

    Étude biochimique des poissons: 1. Variations semestrielles de la teneur en eau et en protéines du tissu musculaire de la truite Arc-en-ciel (Salmo gairdnerii Rich)

    The authors have noted, during the reproductive cycle of the Rainbow trout (Salmo gairdnerii Rich), important variations concerning water and total proteins content. This study shows a great correlation between t...

    J. Gras, R. Reynaud, L. Gamoty, J. Frey, J. C. Henry in Experientia (1967)

  13. No Access

    Article

    Étude biochimique des poissons: 2. Variations semestrielles de la teneur en glycogène, en lipides totaux et en minéraux du tissu musculaire de la truite Arc-en-ciel (Salmo gairdnerii Rich)

    The variations of glycogen, lipids and ashes in muscular tissue of the Rainbow trout (Salmo gairdnerii Rich.) show that during sexual cycle there are 4 periods. Each period corresponds to a particular biological ...

    J. Gras, R. Reynaud, L. Gamoty, J. Frey, J. C. Henry in Experientia (1967)

  14. No Access

    Article

    Die energiereichen Phosphate in der Mäuseniere unter Einwirkung von Inulin und Harnstoff

    K. H. Göggel, G. Hierholzer, J. Kraus… in Pflüger's Archiv für die gesamte Physiolog… (1960)

  15. No Access

    Article

    Antidiurese und Zunahme der Harnkonzentration nach i.v. Vasopressin-Gabe beim Menschen

    J. Frey, L. Kerp, W. Reichardt in Pflüger's Archiv für die gesamte Physiolog… (1960)