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Article
Open AccessMutations of IFT81, encoding an IFT-B core protein, as a rare cause of a ciliopathy
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Article
Influence of buffer composition on protein adsorption on agarose (Sepharose 6B)
The adsorption of albumin on a commercial, high quality agarose (Sepharose® 6B) was studied in different buffers of low pH (4.00) and low molarity (9–11 mM). Eluents containing 0.025% albumin were introduced onto...
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Chapter
Covalent Chromatography
Covalent chromatography is a method for separation of molecules, based on formation of reversible covalent bonds between functional groups in molecules and complementary structures on a stationary solid phase....
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Article
Sweden: Molecular Sieving and Adsorption
Research and development in universities and industry in Sweden has provided methods for separating molecules according to their size, shape and sometimes charge by the molecular sieving effect of gel-forming ...
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Article
Insulin antibodies: Description of specific serum protein localization in a patient with insulin resistant diabetes
1. A patient with insulin resistant diabetes mellitus has been described in detail with emphasis upon the time relationship between prior insulin therapy for mental illness and the subsequent development of ov...
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Article
Separation Methods based on Molecular Sieving and Ion Exclusion
CROSS-LINKED dextran (‘Sephadex’) has been shown to possess properties of a molecular sieve1. The size limits for effective molecular sieving depend on the degree of cross-linking. Molecules or ions capable of en...
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Article
A Method for concentrating Solutes of High Molecular Weight
CONCENTRATION of diluted aqueous solutions of labile solutes of high molecular weight, such as proteins, is often difficult in the laboratory as well as on a technical scale. We wish to present a mild concentr...
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Article
Further studies on corticotropin releasing factor (CRF); corticotropin releasing activity of synthetic peptides
Une fraction peptidique ayant une forte activité d'hypophyso-stimuline à effet corticotrope ou «Corticotropin Releasing Factor» (CRF),in vitro, a été obtenue à partir de la poudre d'hypophyses postérieures de por...
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Article
Gel Filtration as a Method for Purification of Protein-bound Peptides Exemplified by Oxytocin and Vasopressin
WE would like to call attention to a new procedure suitable for purification of simple compounds weakly associated with high molecular weight substances. The method, which was proposed elsewhere by one of us1, is...
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Article
Migration-elution of Zones in an Electrophoresis Column
SO far the preparation of electrophoretically defined fractions by means of zone electrophoresis in powder columns has been performed in two separate steps. Electrophoresis has been succeeded by displacement o...
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Article
Vitamine
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Article
Structure of Bacitracin A
A BACITRACIN polypeptide purified by charcoal chromatography was shown by ultracentrifugation to have a molecular weight of 1,400–1,5001. Recently, by a modified procedure using milder conditions, a peptide of hi...
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Article
A New Method for the Detection of Amino-Acids, Peptides, Proteins and Other Buffering Substances on Paper
DURING the past few years, a large number of papers have appeared dealing with the quantitative determination of amino-acids in paper chromatograms in situ or after elution; but no simple satisfactory general met...