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Article
Open AccessInheritable testicular metabolic memory of high-fat diet causes transgenerational sperm defects in mice
The consumption of energy-dense diets has contributed to an increase in the prevalence of obesity and its comorbidities worldwide. The adoption of unhealthy feeding habits often occurs at early age, prompting ...
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Protocol
Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis
Mitochondria play a central role in metabolic reprograming that occurs in numerous disease conditions. A precise evaluation of the extent of mitochondrial involvement in the metabolic alterations is essential ...
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Article
Different Malabsorptive Obesity Surgery Interventions Result in Distinct Postprandial Amino Acid Metabolomic Signatures
Biliopancreatic diversion with duodenal switch (BPD-DS) is an effective weight loss surgical procedure. Yet, BPD-DS is technically difficult to perform and carries a higher risk of nutrient deficiencies as com...
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Article
The potential of micelleplexes as a therapeutic strategy for osteosarcoma disease
Osteosarcoma (OS) is a rare aggressive bone, presenting low patient survival rate, high metastasis and relapse occurrence, mostly due to multi-drug resistant cells. To surpass that, the use of nanomedicine for...
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Article
Gastric Bypass with Different Biliopancreatic Limb Lengths Results in Similar Post-absorptive Metabolomics Profiles
Roux-en-Y gastric bypass (RYGB) with a long biliopancreatic limb (BPL) was demonstrated to further improve type 2 diabetes (T2D) outcomes. Whether benefits occur at the cost of a negative impact on nutrient ab...
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Article
Open AccessIGF2 role in adrenocortical carcinoma biology
Clinical outcomes of adrenocortical carcinomas (ACC) could be improved by using novel treatment targets based on the recent advances of tumor biology knowledge. Insulin-like growth factor 2 (IGF2) protein expr...
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Article
Fractal Approach for 1H-NMR Spectra Simplification and Data Processing
Nuclear magnetic resonance (NMR) is a powerful instrumental technique suited to characterize and identify organic substances, and has been successfully applied in the analysis of complex matrices such as biolo...
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Article
Response to dietary carbohydrates in European seabass (Dicentrarchus labrax) muscle tissue as revealed by NMR-based metabolomics
Feed optimization is a key step to the environmental and economic sustainability of aquaculture, especially for carnivorous species. Plant-derived ingredients can contribute to reduce costs and nitrogenous eff...
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Article
The effects of the obesogen tributyltin on the metabolism of Sertoli cells cultured ex vivo
Human exposure to environmental contaminants is widespread. Some of these contaminants have the ability to interfere with adipogenesis, being thus considered as obesogens. Recently, obesogens have been singled...
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Protocol
Assessing Sertoli Cell Metabolic Activity
Nuclear magnetic resonance (NMR)-based metabolomics is widely used in the research of metabolic conditions of complex biological systems under various conditions, and its use has been found in the field of mal...
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Protocol
Mitochondrial Bioenergetics by 13C–NMR Isotopomer Analysis
Metabolic reprogramming has been associated to a plethora of diseases, and there has been increased demand for methodologies able to determine the metabolic alterations that characterize the pathological state...
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Article
Putative mechanisms of antitumor activity of cyano-substituted heteroaryles in HeLa cells
Six recently synthesized cyano-substituted heteroaryles, which do not bind to DNA but are highly cytotoxic against the human tumor cell line HeLa, were analyzed for their antitumor mechanisms of action (MOA). ...
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Protocol
NMR Methodologies for Studying Mitochondrial Bioenergetics
Nuclear magnetic resonance (NMR) spectroscopy is a technique with an increasing importance in the study of metabolic diseases. Its initial important role in the determination of chemical structures (1, 2) has ...
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Article
Potentially biologically active new substituted anilides of benzo[2,3-b]thiophene series
New substituted anilides of the heterocyclic series 2, 4, 5, 6, 7 together with the earlier described compounds 1 and 3 (Jarak I et al. (2005) J Med Chem 48:2346), were synthesized from the corresponding heterocy...
