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Open AccessMonkeypox virus genomic accordion strategies
The 2023 monkeypox (mpox) epidemic was caused by a subclade IIb descendant of a monkeypox virus (MPXV) lineage traced back to Nigeria in 1971. Person-to-person transmission appears higher than for clade I or s...
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Article
Open AccessGenotypic study of Chlamydia trachomatis for lymphogranuloma venereum diagnosis in rectal specimens from men who have sex with men: a cost-effectiveness analysis
The significant proportion of asymptomatic patients and the scarcity of genotypic analysis of lymphogranuloma venereum (LGV), mainly among men who have sex with men (MSM), triggers a high incidence of underdia...
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Article
Open AccessSARS-CoV-2-reactive IFN-γ-producing CD4+ and CD8+ T cells in blood do not correlate with clinical severity in unvaccinated critically ill COVID-19 patients
We examined the relationship between peripheral blood levels of SARS-CoV-2 S (Spike protein)1/M (Membrane protein)-reactive IFN-γ-producing CD4+ and CD8+ T cells, serum levels of biomarkers of clinical severity, ...
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Article
Open AccessCombined kinetic analysis of SARS-CoV-2 RNAemia, N-antigenemia and virus-specific antibodies in critically ill adult COVID-19 patients
Combined kinetic analysis of plasma SARS-CoV-2 RNAemia, Nucleocapsid (N)-antigenemia and virus-specific antibodies may help ascertain the role of antibodies in preventing virus dissemination in COVID-19 patien...
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Article
Performance comparison of a flow cytometry immunoassay for intracellular cytokine staining and the QuantiFERON® SARS-CoV-2 test for detection and quantification of SARS-CoV-2-Spike-reactive-IFN-γ-producing T cells after COVID-19 vaccination
We compared the performance of an in-house-developed flow cytometry assay for intracellular cytokine staining (FC-ICS) and a commercially-available cytokine release assay (the QuantiFERON® SARS-CoV-2 Test [QF]...
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Article
Open AccessNeutralizing antibodies against SARS-CoV-2 variants of concern elicited by the comirnaty COVID-19 vaccine in nursing home residents
Immunosenescence may impact the functionality and breadth of vaccine-elicited humoral immune responses. The ability of sera to neutralize the SARS-CoV-2 spike protein (S) from Beta, Gamma, Delta, and Epsilon v...
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Article
Open AccessMulti-body-site colonization screening cultures for predicting multi-drug resistant Gram-negative and Gram-positive bacteremia in hematological patients
To investigate the multi-drug resistant bacteria (MDRB) colonization rate in hematological patients hospitalized for any cause using a multi-body-site surveillance approach, and determine the extent to which t...
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Article
Open AccessPerformance of a flow cytometry-based immunoassay for detection of antibodies binding to SARS-CoV-2 spike protein
The performance of a laboratory-developed IgG/IgA flow cytometry-based immunoassay (FCI) using Jurkat T cells stably expressing full-length native S protein was compared against Elecsys electrochemiluminiscent...
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Article
The first wave of the COVID-19 epidemic in Spain was associated with early introductions and fast spread of a dominating genetic variant
The coronavirus disease 2019 (COVID-19) pandemic has affected the world radically since 2020. Spain was one of the European countries with the highest incidence during the first wave. As a part of a consortium...
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Article
Performance of a MALDI-TOF mass spectrometry-based method for rapid detection of third-generation oxymino-cephalosporin-resistant Escherichia coli and Klebsiella spp. from blood cultures
We optimized and prospectively evaluated a simple MALDI-TOF MS-based method for direct detection of third-generation oxymino-cephalosporin resistance (3rd CephR) in Escherichia coli and Klebsiella spp. from blood...
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Article
Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemiluminescent immunoassays
Whether antibody levels measured by commercially available enzyme or chemiluminescent immunoassays targeting the SARS-CoV-2 spike (S) protein can act as a proxy for serum neutralizing activity remains to be es...
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Article
Vancomycin MICs and risk of complicated bacteremia by glycopeptide-susceptible Staphylococcus aureus
Vancomycin (VAN) minimum inhibitory concentrations (MICs) at the upper end of the susceptible range for Staphylococcus aureus (S. aureus), as measured by the Etest method, have been associated with poor clinical ...
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Article
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) proteomic profiling of cerebrospinal fluid in the diagnosis of enteroviral meningitis: a proof-of-principle study
The use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for diagnosing viral infections by directly testing clinical specimens has not previously been explored. I...
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Evolutionary Ethnobotanical Studies of Incipient Domestication of Plants in Mesoamerica
Human cultures that occupied the area currently known as Mesoamerica developed a broad repertory of technologies for managing the biotic resources and ecosystems of the surrounding areas they lived. These tech...
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Article
Open AccessTEK, local perceptions of risk, and diversity of management practices of Agave inaequidens in Michoacán, Mexico
Mescal production is the main economic activity associated to agaves in Mexico, which involves 53 species mostly harvested from forests. The increasing mescal demand has influenced risk in both agave populatio...
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Article
Population Dynamics and Sustainable Management of Mescal Agaves in Central Mexico: Agave potatorum in the Tehuacán-Cuicatlán Valley
Population Dynamics and Sustainable Management of Mescal Agaves in Central Mexico: Agave potatorum in the Tehuacán-Cuicatlán Valley. A total of 37 agave species ar...
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Article
Open AccessVulnerability and risk management of Agave species in the Tehuacán Valley, México
Our study analysed the vulnerability of the useful Agave species of the Tehuacán Valley, Mexico, considering ecological, cultural and economic aspects, and management types. We hypothesized that management intens...
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Article
Open AccessInfluence of traditional markets on plant management in the Tehuacán Valley
The Tehuacán Valley, Mexico is a region with exceptionally high biocultural richness. Traditional knowledge in this region comprises information on nearly 1,600 plant species used by local peoples to satisfy t...
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Article
Plant Management in the Tehuacán-Cuicatlán Valley, Mexico1
Plant Management in the Tehuacán-Cuicatlán Valley, Mexico. Plant management types currently practiced in the Tehuacán-Cuicatlán Valley, México, were documented and analyzed based on ethnobotanical studies conduct...
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Article
Traditional Knowledge and Useful Plant Richness in the Tehuacán–Cuicatlán Valley, Mexico
Traditional Knowledge and Useful Plant Richness in the Tehuacán–Cuicatlán Valley, Mexico. This study systematizes ethnobotanical information about the interactions between people and plan...