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Article
Genome analysis of Zoysia japonica ‘Yaji’ cultivar using PacBio long-read sequencing
The Zoysia japonica plant is a warm-season turfgrass and a horticultural crop used in various places and an important genetic resource because it is more resilient to stress from high temperature, drought, coasta...
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Article
Risk assessment of genetically modified zoysiagrasses (Zoysia japonica) in a zebrafish model
Genetically modified (GM) plants have been mainly advanced for the mass production of agricultural plants. However, there are concerns about GM crops which cause side effects on ecosystems and human beings. Ze...
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Article
Pollen-mediated flow of bar gene in transgenic herbicide-resistant turf grass Zoysia japonica
Weed control can be most effectively achieved through the use of herbicide-resistance transgene. A preliminary study bar-transgenic Zoysia japonica posed no serious risk on the unintended escape of the transgene ...
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Article
Overexpression of ATHG1/AHL23 and ATPG3/AHL20, Arabidopsis AT-hook motif nuclear-localized genes, confers salt tolerance in transgenic Zoysia japonica
Zoysia japonica Steud. is a native turfgrass in the Korean peninsula and is used worldwide. Abnormal conditions of soil salinity inhibit plant growth and adversely affect the quality of the turfgrass. AT-hook mot...
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Article
A novel basic helix-loop-helix transcription factor, ZjICE2 from Zoysia japonica confers abiotic stress tolerance to transgenic plants via activating the DREB/CBF regulon and enhancing ROS scavenging
ZjICE2 works as a positive regulator in abiotic stress responses and ZjICE2 is a valuable genetic resource to improve abiotic stress tolerance in the molecular breeding program of Zoysia japonica.
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Article
Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass
Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of fre...
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Article
Overexpression of ICE1, a Regulator of Cold-Induced Transcriptome, Confers Cold Tolerance to Transgenic Zoysia japonica
Numerous genes are known to regulate the cold response in plants. The ICE1 gene is a regulator of coldinduced transcriptome playing an important role in cold tolerance in Arabidopsis thaliana. Here, the Arabidops...
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Article
Resistance of transgenic zoysiagrass overexpressing the zoysiagrass class II chitinase gene Zjchi2 against Rhizoctonia solani AG2-2 (IV)
Zoysiagrass (Zoysia japonica Steud.) is an important turfgrass species used in golf courses and athletic fields. However, zoysiagrass is susceptible to large patch disease caused by Rhizoctonia solani AG2-2 (IV)....
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Article
Molecular cloning and characterization of chitinase genes from zoysiagrass (Zoysia Japonica Steud.)
Zoysiagrass (Zoysia Japonica Steud.) is used frequently in golf courses and athletic fields. However, Zoysiagrass suffers from large-patch disease caused by Rhizoctonia solani AG2-2 (IV), which results in physica...
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Article
Investigation into effects of transgenic glufosinate-resistant Zoysia grasses with herbicide application on bacterial communities under field conditions
Zoysia grass (Zoysia japonica Steud.) is a commercially valuable turfgrass that is popularly cultivated for use in sports and recreational environments. A field study was conducte...
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Article
Homologous expression of cytosolic dehydroascorbate reductase increases grain yield and biomass under paddy field conditions in transgenic rice (Oryza sativa L. japonica)
Dehydroascorbate reductase (DHAR, EC 1.8.5.1) maintains redox pools of ascorbate (AsA) by recycling oxidized AsA to reduced AsA. To investigate whether DHAR affects rice yield under normal environmental condit...
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Article
Mitochondrial activity in illuminated leaves of chlorophyll-deficient mutant rice (OsCHLH) seedlings
The rice CHLH gene encodes the Mg2+-chelatase H subunit, which is involved in chlorophyll biosynthesis. Growth of the chlorophyll-deficient oschlh mutant is supported by mitochondrial activity. In this study, we ...
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Article
Assessment of Phosphinothricin Acetyltransferase (PAT) Degradation From Transgenic Zoysiagrass Digested with Simulated Gastric Fluid (SGF)
The in vitro pepsin digestion assay is the international standard for assessing the safety or risk of novel proteins newly produced in transgenic crops. However, this protocol, based on the degradation of prot...
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Article
Evaluation of Viability, Shedding Pattern, and Longevity of Pollen from Genetically Modified (GM) Herbicide-tolerant and Wild-type Zoysiagrass (Zoysia japonica Steud.)
Knowledge about pollen viability is important when evaluating the risk of genetically modified (GM) plants. Here, staining via iodine potassium iodide (IKI) or triphenyl tetrazolium chloride (TTC) could not di...
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Article
Breeding of four-leaf white clover (Trifolium repens L.) through 60Co gamma-ray irradiation
Four-leaf white clover is not found easily in nature due to its low appearance rate (1 in 10,000). Because people believe that it brings good luck and like to either keep it or present it to a loved one, it ha...
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Article
Rice phot1a mutation reduces plant growth by affecting photosynthetic responses to light during early seedling growth
The aim of this work was to characterize the phot1 mutant of rice during early seedling growth in various light conditions. We isolated the rice T-DNA insertion mutant phot1a-1 and compared it to the Tos17 insert...
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Article
Identification of the ADP-glucose pyrophosphorylase isoforms essential for starch synthesis in the leaf and seed endosperm of rice (Oryza sativa L.)
ADP-glucose pyrophosphorylase (AGP) catalyzes the first committed step of starch biosynthesis in higher plants. To identify AGP isoforms essential for this biosynthetic process in sink and source tissues of ri...
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Article
Morphological alterations by ectopic expression of the rice OsMADS4 gene in tobacco plants
OsMADS4, a rice MADS-box gene, is a member of the GLO/PI family that specifies the identity of petals and stamens in combination with other MADS-box genes. We report here the ectopic expression of OsMADS4 fused t...
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Article
Generation of T-DNA Tagging Lines with a Bidirectional Gene Trap Vector and the Establishment of an Insertion-Site Database
We have developed a binary T-DNA vector, pGA2717, that contains the promoter-less β-glucuronidase (gus) gene adjacent to the right border and the promoter-less green fluorescence protein (gfp) gene next to the le...
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Article
A complete sequence of the pGA1611 binary vector
We report the nucleotide sequence of the binary vector pGA1611, which is used for the transformation of foreign DNA into rice. This vector is 13,476 bp long. The 5577- bp T- DNA region consists of a 1987- bp u...