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Protocol
Co-immunoprecipitation for Assessing Protein–Protein Interactions in Agrobacterium-Mediated Transient Expression System in Nicotiana benthamiana
The characterization of protein–protein interactions (PPI) often provides functional information about a target protein. Yeast-two-hybrid (Y2H) and luminescence/fluorescence-based detections, therefore, have b...
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Article
Open AccessNorthward expansion trends and future potential distribution of a dragonfly Ischnura senegalensis Rambur under climate change using citizen science data in South Korea
Citizen science is becoming a mainstream approach of baseline data collection to monitor biodiversity and climate change. Dragonflies (Odonata) have been ranked as the highest priority group in biodiversity mo...
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Protocol
High-Throughput Targeted Transcriptional Profiling of Defense Genes Using RNA-Mediated Oligonucleotide Annealing, Selection, and Ligation with Next-Generation Sequencing in Arabidopsis
Tracking RNA transcription has been one of the most powerful tools to gain insight into the biological process. While a wide range of molecular methods such as northern blotting, RNA-seq, and quantitative RT-P...
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Protocol
Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae
Changes in chromatin accessibility are an important aspect of the molecular changes that occur in eukaryotic cells responding to stress, and they appear to play a critical role in stress-induced transcriptiona...
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Article
Correction: Corrigendum: CRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity
Nature Communications 3: Article number: 1297 (2012); Published: 18 December 2012; Updated: 26 February 2013. The original version of this Article contained a typographical error in the spelling of the author ...
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Article
CRT1 is a nuclear-translocated MORC endonuclease that participates in multiple levels of plant immunity
Arabidopsis thaliana CRT1 (compromised for recognition of Turnip Crinkle Virus) was previously shown to be required for effector-triggered immunity. Sequence analyses previously revealed that CRT1 contains the AT...
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Article
Salicylic acid-inducible Arabidopsis CK2-like activity phosphorylates TGA2
We demonstrate that TGA2, TGA5 and TGA6, and TGA3 to a lesser extent, are phosphorylated by an activity in rabbit reticulocytes. Using deletion and point mutagenesis of TGA2, three amino acid (aa) residues, 11 Se...
