13 Result(s)
within
Hong Feng
Biotechnology
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Article
Open AccessTuning transcription factor DegU for develo** extracellular protease overproducer in Bacillus pumilus
Global transcription machinery engineering (gTME) is an effective approach employed in strain engineering to rewire gene expression and reshape cellular metabolic fluxes at the transcriptional level.
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Article
Precisely map** a major QTL for grain weight on chromosome 5B of the founder parent Chuanmai42 in the wheat-growing region of southwestern China
QTgw.saas-5B was validated as a major thousand-grain weight-related QTL in a founder parent used for wheat breeding and then precisely mapped to a 0.6 cM interval.
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Characterization of a Protease Hyper-Productive Mutant of Bacillus pumilus by Comparative Genomic and Transcriptomic Analysis
Bacillus pumilus BA06 has great potential for the production of alkaline proteases. To improve the protease yield, classical mutagenesis to combine the physical and chemical mutagens was performed to obtain a pro...
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A novel DNA methylation motif identified in Bacillus pumilus BA06 and possible roles in the regulation of gene expression
Single-molecule real-time (SMRT) sequencing can be used to identify a wide variety of chemical modifications of the genome, such as methylation. Here, we applied this approach to identify N6-methyl-adenine (m6...
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Open AccessRecombinant expression and biochemical characterization of a novel keratinase BsKER71 from feather degrading bacterium Bacillus subtilis S1-4
Bacillus subtilis S1-4, isolated from chicken feather could efficiently degrade feathers by secreting several extracellular proteases. In order to get insight into the individual protease involved in keratin hydr...
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Open AccessEngineering Bacillus pumilus alkaline serine protease to increase its low-temperature proteolytic activity by directed evolution
Mesophilic alkaline serine proteases from various bacteria have been commercially applied in a range of industries owing to their high catalytic efficiency and wide substrate specificity. However, these protea...
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Identification and characterization of a novel antioxidant peptide from feather keratin hydrolysate
To improve the potential value of feather, which is a valuable protein resource, we have separated and identified antioxidant peptide(s) from feather hydrolysate.
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Transcriptional analysis of adaptation to high glucose concentrations in Zymomonas mobilis
The ethanologenic bacterium Zymomonas mobilis is usually tolerant to high concentrations of glucose. The addition of sorbitol decreases the lag phase and increases ethanol yield and productivity of the bacteria i...
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A Simple and Reproducible Method for Directed Evolution: Combination of Random Mutation with dITP and DNA Fragmentation with Endonuclease V
An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used ...
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Article
Changes in functional diversity of soil microbial community with addition of antibiotics sulfamethoxazole and chlortetracycline
Potential effects of antibiotics on agricultural soil microflora have recently become increasing concerns with antibiotic-contaminated biosolid now being used in agricultural land. However, changes of soil mic...
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Substrate Specificity and Thermostability of the Dehairing Alkaline Protease from Bacillus pumilus
An alkaline protease (DHAP) from Bacillus pumilus has shown great potential in hide dehairing. To get better insights on its catalytic properties for application, the substrate specificity and thermostability wer...
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Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif
A novel bacterial cell-surface display system was developed in Escherichia coli using omp1, a hypothetical outer membrane protein of Zymomonas mobilis. By using this system, we successfully expressed β-amylase ge...
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Cloning and Expression of an α-Amylase Gene from Phanerochaete chrysosporium
Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an α-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment r...
