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Article
Highly Sensitive Simultaneous Bioluminescent Measurement of Acetate Kinase and Pyruvate Phosphate Dikinase Activities Using a Firefly Luciferase-Luciferin Reaction and Its Application to a Tandem Bioluminescent Enzyme Immunoassay
We have developed a simultaneous bioluminescent measurement of acetate kinase (AK) and pyruvate phosphate dikinase (PPDK) activities and its application to a tandem enzyme immunoassay. The principle of the pro...
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Article
The Immunoassay of Methotrexate by Capillary Electrophoresis with Laser-induced Fluorescence Detection
Immunoassay is widely employed as a highly sensitive, specific analytical method for hormones and drugs in biological samples. A technique utilizing capillary electrophoresis with laser-induced fluorescence de...
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Article
Simultaneous Analysis of Two-Site Gene Polymorphisms Using Time-Resolved Fluorescence Immunoassay
We have developed a method for the simultaneous analysis of two-site gene polymorphisms. The principle developed by us is that two amplicons are produced by duplex PCR using two different primer sets (digoxige...
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Article
Chemiluminescent Assay for Catecholamines by the Generation of Hydrogen Peroxide in Basic Solution and the Use of Isoluminol-Microperoxidase
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Article
Bioluminescent PCR-RFLP Enzyme-Linked Immunosorbent Assay for Analysis of Vitamin D Receptor Gene Polymorphism
We developed a sensitive and rapid PCR-RFLP ELISA using acetate kinase (AK) and firefly luciferase as a detection system. AK used as a label enzyme could sensitively be detected by bioluminescent assay using t...
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Article
Enhanced Resolution in the Capillary Electrophoretic Separation of Double-Stranded DNA Using Dextran Sulfate and Its Application to the Analysis of PCR Products
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Article
Chemiluminescent Enzyme Immunoassay Using an Alkaline Phosphatase Label and an Indolyl Phosphate Substrate
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Article
Chemiluminescent and Bioluminescent Assays for Polyadenylic Acid and Applications to DNA Probe Assay and Immunoassay
We developed a chemiluminescent (CL) assay for polyadenylic acid (poly A) based on the enzymatic cycling reaction following the conversion to ATP by pyruvate kinase/polynucleotide phosphoryrase enzyme reaction...
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Article
Fluorescent Enzyme Immunoassay of Free Thyroxin Using Glucose Oxidase as Enzyme Label
A fluorescent enzyme immunoassay for free thyroxin using glucose oxidase as the label enzyme has been developed. Double antibody solid phase method was used. After separation of bound and free fractions the gl...
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Article
Chemiluminescent Enzyme Immunoassay A Review
Highly sensitive chemiluminescent enzyme immunoassays developed in our laboratory are reviewed. Oxidases, peroxidase and glucose oxidase, generating H2O2 can be assayed by isoluminol/microperoxidase or peroxy-oxa...
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Article
Strong gusts in the lowest 250-m layer over the city of Tokyo
From the architectural point of view, the vertical distribution of strong gusts over large cities is very important. Although special instrumentation for very strong winds is required, the available recording ...
