Hepatitis C
Methods and Protocols
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Protocol
iPS Cell Differentiation into Brain Microvascular Endothelial Cells
The blood–brain barrier is a tissue structure that modulates the selective entry of molecules into the brain compartment. This barrier offers protection to the brain microenvironment from toxins or any fluctua...
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Article
Biological activity-based modeling identifies antiviral leads against SARS-CoV-2
Computational approaches for drug discovery, such as quantitative structure–activity relationship, rely on structural similarities of small molecules to infer biological activity but are often limited to ident...
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Article
Open AccessEmetine inhibits Zika and Ebola virus infections through two molecular mechanisms: inhibiting viral replication and decreasing viral entry
The re-emergence of Zika virus (ZIKV) and Ebola virus (EBOV) poses serious and continued threats to the global public health. Effective therapeutics for these maladies is an unmet need. Here, we show that emet...
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Article
Zika virus directly infects peripheral neurons and induces cell death
Zika virus infection is associated with neurological disorders, yet few studies have directly examined its impact on the peripheral nervous system. Oh et al. show that Zika virus can infect peripheral neurons in ...
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Article
Identification of small-molecule inhibitors of Zika virus infection and induced neural cell death via a drug repurposing screen
A high-throughput screen of preclinical, investigational and FDA-approved drugs identifies compounds that possess antiviral and neuroprotective effects against Zika virus infection in human neural progenitor c...
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Open AccessZika virus and neural developmental defects: building a case for a cause
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Article
Targeting host cofactors to inhibit viral infection
The majority of FDA-approved drugs indicated for the treatment of viral infections are inhibitors of viral proteins, of which the emergence of resistant strains is a major concern. This issue is exacerbated as...
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Book
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Selection and Characterization of Drug-Resistant HCV Replicons In Vitro with a Flow Cytometry-Based Assay
Because HCV RNA-dependent RNA polymerase is error-prone and the viral RNA has a high turnover rate, the genetic diversity of HCV is very high both in vitro and in vivo. The mutation rate in long-term replicon cul...
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Reference Work Entry In depth
Proteomics in Human‐Pathogen Interactions
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Article
Sam68, RNA helicase A and Tap cooperate in the post-transcriptional regulation of human immunodeficiency virus and type D retroviral mRNA
Unlike cellular mRNA, retroviral mRNA bypasses the tight coupling of the splicing and nuclear export steps to allow the export of intron-containing viral RNA transcripts to the cytoplasm. Two distinct nuclear ...
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Article
Inhibition of HIV replication by dominant negative mutants of Sam68, a functional homolog of HIV-1 Rev
The HIV-1 Rev protein facilitates the nuclear export of mRNA containing the Rev response element (RRE) through binding to the export receptor CRM-1. Here we show that a cellular nuclear protein, Sam68 (Src-ass...
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Article
Functional interaction of the HTLV-1 transactivator Tax with activating transcription factor-4 (ATF4)
The Tax protein of the Human T-cell Leukemia Virus (HTLV) activates the expression of viral mRNA through a three 21 bp repeat enhancer located within the HTLV-1 LTR. Since Tax does not bind to the 21 bp DNA re...
