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Chapter
ADP-Ribose
ADP-ribosylation of proteins was first detected as a modification of nuclear proteins by polymeric ADP-ribose residues derived from NAD+. Subsequently, the field developed into three destinct sections: (i) Possib...
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A Role of Poly(ADP-Ribosyl)ation in Carcinogen-Inducible Transient DNA Amplification
DNA amplification is viewed as an adaptive cellular response to environmental changes and genotoxic stress (1). It is an important mechanism in the development of drug resistance (1–4) and seems to play signif...
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Chapter
Phospho ADP-Ribosylation and Phospho Adenylation of Proteins
In 1941 the late Fritz Lipmann formulated his now famous concept of energy-rich bonds (1). Although he developed his thesis for phosphate compounds, it has proven extremely fruitful in other areas as well. Whe...
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Divergence of Poly(ADP-ribose) Polymerase Activity, Poly(ADP-ribose) Levels and the Effect of Polymerase Inhibitors in Differentiating 3T3-L1 Preadipocytes
Modification of nuclear proteins by poly(ADP-ribose) has been implicated as an essential step in the differentiation of a number of different systems. However, in some systems a transient rise of poly(ADP-ribo...
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Chapter
Cytotoxicity of TPA-Stimulated Granulocytes and Inhibitors of ADP-Ribosylation
Reactive oxygen species as derived from stimulated phagocytes are able to induce DNA fragmentation (1, 2). Since fragmented DNA is known to activate poly(ADP-ribose) polymerase (3), it could be expected that t...
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Poly(ADP-Ribose) Polymerase: Determination of Content from TCA-Precipitated Cells, Existence of Species-Specific Isoforms and Changes During the Growth Cycle
Poly(ADP-ribose) polymerase itself is one of the major acceptors of poly(ADP-ribose) residues under conditions of DNA fragmentation in vitro (1) and in vivo (2, 3). For a better understanding of the involvement o...
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Book
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Mono(ADP-Ribosyl)ation and Phospho(ADP-Ribosylation)Reactions in Eukaryotic Cells
The existence in vivo of mono(ADPR) protein conjugates has been described by this and other laboratories [1, 2]. Also, a subfractionation of endogenous mono(ADPR) conjugates was introduced by Bredehorst and co...
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Chapter
Mono(ADP-Ribosyl) Histone H1 in Alkylated Hepatoma Cells: Unusual Acceptor Site
Treatment of cells with DNA-damaging agents like UV irradiation or alkylating chemicals is known to greatly stimulate mono- and poly(ADPR) formation [1, 2] and turnover [3, 4]. Although there are many attempts...
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Chapter
ADP-Ribosylation of Proteins in Normal and Neoplastic Tissues
In 1966, Mandel and coworkers in Strasbourg described an enzyme located in the nucleus of chicken liver, which is able to transfer active ADPR groups from NAD to form a homopolymer (1). In this polymer ADPR re...
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Article
Divergent action mechanism of cAMP and dibutyryl cAMP on cell proliferation and macromolecular synthesis in HeLa S3 cultures
In HeLa S3 cultures, exogenous cAMP and its dibutyryl derivative (DBcAMP) produced divergent effects with respect to glycogen levels as well as to incorporation of labeled precursors into nucleic acids, concen...
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Article
The Cytostatic Action of Extracellular NAD in Tumour-bearing Mice
Repeated injections of NAD led to a dose-dependent inhibition of cell proliferation in tumour-bearing mice (Ehrlich ascites carcinoma and a murine mastocytoma). NAD proved clearly superior to other adenine nuc...
