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Article
Comparative nutritional value of Jatropha curcas protein isolate and soy protein isolate in common carp
Jatropha seed cake (JSC) is an excellent source of protein but does contain some antinutritional factors (ANF) that can act as toxins and thus negatively affect the growth and health status of fish. While this...
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Book
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Book
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Chapter
Determination of Condensed Tannins (Proanthocyanidins)
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Chapter
Gallotannin Determination Using HPLC
Pipette 1 ml of supernatant A (see Section 5) into the above mentioned culture tubes, remove acetone in a vacuum oven adjusted at 40°C (pressure 300 mbar). It takes about 3 h to remove acetone. Acetone can also b...
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Chapter
Treatment of Plant Material, Extraction of Tannins, and an Overview of Tannin Assays Presented in the Manual
Tannins are naturally occurring polyphenolic substances with various molecular weights and a variable complexity. These are chemically not well-defined substances but rather a group of substances with the abil...
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Chapter
Tannin Bioassay
Dried leaves should be passed through a 1 mm sieve.
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Chapter
Simplified Radiolabelled BSA Precipitation Method
The above radiolabelled method hass been simplified to eliminate the more difficult procedures of relatively high speed (13,000 g) centrifugation and manipulations of small volumes of radiochemical solutions by b...
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Chapter
Determination of Protein-Precipitable Phenolics
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Chapter
References
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Chapter
Measurement of Total Phenolics and Tannins Using Folin-Ciocalteu Method
The method for total phenol is useful in order to know the efficiency of extraction of phenolics in solvents. This method can be coupled with the use of insoluble matrix, polyvinyl polypyrrolidone (PVPP; binds...
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Chapter
Radial Diffusion Assay
Type I, gel point 36°C (Sigma A-6013), bovine serum albumin (fraction V) (Sigma A-3350), glacial acetic acid, ascorbic acid and sodium hydroxide.
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Chapter
Gallotannin Determination by Rhodanine Assay
The plant material was extracted as described in Section 2.2.4. The supernatant is designated as supernatant A.
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Chapter
Radiolabelled BSA Precipitation Method
This is the most sensitive method for determining amounts of protein precipitated by a tannin. It is also useful for running competitive binding assay to characterize tannin-protein interactions. The method de...
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Chapter
Determination of Hydrolysable Tannins (Gallotannins and Ellagitannins) after Reaction with Potassium Iodate
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Chapter
Characterization of Phenolic Compounds by Thin Layer Chromatography (TLC)
About 500 g of the shade dried plant material should be ground first to pass a 2 mm screen. All the ground material including those parts remaining inside the mill should be taken, mixed well and approximately...
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Chapter
Protein-Binding Capacity by Filter Paper Assay
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Chapter
Summary of Results from the First Phase of the Co-Ordinated Research Project
This CRP was initiated in 1999. The Second Research Coordination Meeting was held from 19 to 23 November 2001 at Centro de Energia Nuclear na Agricultura (CENA), Universidade de Sao Paulo, Piracicaba, Brazil, ...
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Chapter
Chemical, Protein Precipitation and Bioassays for Tannins, Tannin Levels and Activity in Unconventional Feeds, and Efects and Fate of Tannins
Animal production systems in the tropical and subtropical countries utilize a wide range of feedstuffs; the main amongst these being the crop and industrial by-products, grasses, legumes, trees and shrubs. Tre...