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    Article

    Comparative nutritional value of Jatropha curcas protein isolate and soy protein isolate in common carp

    Jatropha seed cake (JSC) is an excellent source of protein but does contain some antinutritional factors (ANF) that can act as toxins and thus negatively affect the growth and health status of fish. While this...

    Sunil Nepal, Vikas Kumar, Harinder P. S. Makkar in Fish Physiology and Biochemistry (2018)

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    Book

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    Book

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    Chapter

    Determination of Condensed Tannins (Proanthocyanidins)

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Gallotannin Determination Using HPLC

    Pipette 1 ml of supernatant A (see Section 5) into the above mentioned culture tubes, remove acetone in a vacuum oven adjusted at 40°C (pressure 300 mbar). It takes about 3 h to remove acetone. Acetone can also b...

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Treatment of Plant Material, Extraction of Tannins, and an Overview of Tannin Assays Presented in the Manual

    Tannins are naturally occurring polyphenolic substances with various molecular weights and a variable complexity. These are chemically not well-defined substances but rather a group of substances with the abil...

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Tannin Bioassay

    Dried leaves should be passed through a 1 mm sieve.

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Simplified Radiolabelled BSA Precipitation Method

    The above radiolabelled method hass been simplified to eliminate the more difficult procedures of relatively high speed (13,000 g) centrifugation and manipulations of small volumes of radiochemical solutions by b...

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Determination of Protein-Precipitable Phenolics

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    References

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Measurement of Total Phenolics and Tannins Using Folin-Ciocalteu Method

    The method for total phenol is useful in order to know the efficiency of extraction of phenolics in solvents. This method can be coupled with the use of insoluble matrix, polyvinyl polypyrrolidone (PVPP; binds...

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Radial Diffusion Assay

    Type I, gel point 36°C (Sigma A-6013), bovine serum albumin (fraction V) (Sigma A-3350), glacial acetic acid, ascorbic acid and sodium hydroxide.

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Gallotannin Determination by Rhodanine Assay

    The plant material was extracted as described in Section 2.2.4. The supernatant is designated as supernatant A.

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Radiolabelled BSA Precipitation Method

    This is the most sensitive method for determining amounts of protein precipitated by a tannin. It is also useful for running competitive binding assay to characterize tannin-protein interactions. The method de...

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Determination of Hydrolysable Tannins (Gallotannins and Ellagitannins) after Reaction with Potassium Iodate

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Characterization of Phenolic Compounds by Thin Layer Chromatography (TLC)

    About 500 g of the shade dried plant material should be ground first to pass a 2 mm screen. All the ground material including those parts remaining inside the mill should be taken, mixed well and approximately...

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Protein-Binding Capacity by Filter Paper Assay

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Summary of Results from the First Phase of the Co-Ordinated Research Project

    This CRP was initiated in 1999. The Second Research Coordination Meeting was held from 19 to 23 November 2001 at Centro de Energia Nuclear na Agricultura (CENA), Universidade de Sao Paulo, Piracicaba, Brazil, ...

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)

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    Chapter

    Chemical, Protein Precipitation and Bioassays for Tannins, Tannin Levels and Activity in Unconventional Feeds, and Efects and Fate of Tannins

    Animal production systems in the tropical and subtropical countries utilize a wide range of feedstuffs; the main amongst these being the crop and industrial by-products, grasses, legumes, trees and shrubs. Tre...

    Harinder P. S. Makkar in Quantification of Tannins in Tree and Shrub Foliage (2003)