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Article
Open AccessDeconwolf enables high-performance deconvolution of widefield fluorescence microscopy images
Microscopy-based spatially resolved omic methods are transforming the life sciences. However, these methods rely on high numerical aperture objectives and cannot resolve crowded molecular targets, limiting the...
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Article
Open AccessSuper-resolution microscopy reveals that Na+/K+-ATPase signaling protects against glucose-induced apoptosis by deactivating Bad
Activation of the apoptotic pathway is a major cause of progressive loss of function in chronic diseases such as neurodegenerative and diabetic kidney diseases. There is an unmet need for an anti-apoptotic dru...
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Article
Open AccessInactivation of mediator complex protein 22 in podocytes results in intracellular vacuole formation, podocyte loss and premature death
Podocytes are critical for the maintenance of kidney ultrafiltration barrier and play a key role in the progression of glomerular diseases. Although mediator complex proteins have been shown to be important fo...
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Article
Open AccessOuabain Modulates the Functional Interaction Between Na,K-ATPase and NMDA Receptor
The N-methyl-D-aspartate (NMDA) receptor plays an essential role in glutamatergic transmission and synaptic plasticity and researchers are seeking for different modulators of NMDA receptor function. One possib...
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Article
A molecular mechanism explaining albuminuria in kidney disease
Mammalian kidneys constantly filter large amounts of liquid, with almost complete retention of albumin and other macromolecules in the plasma. Breakdown of the three-layered renal filtration barrier results in...
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Article
Open AccessAuthor Correction: Extracellular nanovesicles released from the commensal yeast Malassezia sympodialis are enriched in allergens and interact with cells in human skin
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.
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Article
Open AccessCoro2b, a podocyte protein downregulated in human diabetic nephropathy, is involved in the development of protamine sulphate-induced foot process effacement
Podocytes have an important role in the pathogenesis of diabetic nephropathy (DN). Podocyte foot process effacement, mediated largely by the actin-based cytoskeleton of foot processes, is commonly detected in ...
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Article
Open AccessExtracellular nanovesicles released from the commensal yeast Malassezia sympodialis are enriched in allergens and interact with cells in human skin
Malassezia sympodialis is a dominant commensal fungi in the human skin mycobiome but is also associated with common skin disorders including atopic eczema (AE). M. sympodialis releases extracellular vesicles, des...
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Article
Open AccessCerium oxide nanoparticles inhibit differentiation of neural stem cells
Cerium oxide nanoparticles (nanoceria) display antioxidant properties and have shown cytoprotective effects both in vitro and in vivo. Here, we explored the effects of nanoceria on neural progenitor cells using t...
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Article
Super-resolution structured illumination fluorescence microscopy of the lateral wall of the cochlea: the Connexin26/30 proteins are separately expressed in man
Globally 360 million people have disabling hearing loss and, of these, 32 million are children. Human hearing relies on 15,000 hair cells that transduce mechanical vibrations to electrical signals in the audit...
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Article
Open AccessSuper-resolution microscopy reveals γ-secretase at both sides of the neuronal synapse
The transmembrane protein assembly γ-secretase is a key protease in regulated intramembrane processing (RIP) of around 100 type-1 transmembrane proteins. Importantly, it has a pathological role in Alzheimer di...
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Article
Glial origin of mesenchymal stem cells in a tooth model system
In the mouse, nerve-associated peripheral glial cells give rise to mesenchymal stem cells that contribute to the development and growth of adult teeth.
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Article
Open AccessNanoscale elucidation of Na,K-ATPase isoforms in dendritic spines
The dimensions of neuronal synapses suggest that optical super-resolution imaging methods are necessary for thorough investigation of protein distributions and interactions. Nanoscopic evaluation of neuronal s...
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Chapter and Conference Paper
Modelling of Safety-Related Timing Constraints for Automotive Embedded Systems
Timing and functional safety are important aspects when develo** automotive embedded systems. The two aspects have however mostly been studied as separate aspects, up to now. This paper presents an investiga...
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Article
Open AccessAutomated data extraction from general practice records in an Australian setting: Trends in influenza-like illness in sentinel general practices and emergency departments
Influenza intelligence in New South Wales (NSW), Australia is derived mainly from emergency department (ED) presentations and hospital and intensive care admissions, which represent only a portion of influenza...
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Article
Open AccessSpatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy
The Na+,K+-ATPase plays an important role for ion homeostasis in virtually all mammalian cells, including neurons. Despite this, there is as yet little known about the isoform specific distribution in neurons.
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Article
Characterization, Production, and Purification of Carnocin H, a Bacteriocin Produced by Carnobacterium 377
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Chapter and Conference Paper
Flow Profiling in Microstructures with Micrometer Resolution
We demonstrate the determination of hydrodynamic flow properties in silicon channel microstructures by Fluorescence Correlation Spectroscopy (FCS). Flow velocities up to 10 mm/s and a parabolic flow profile we...
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Chapter and Conference Paper
Single Molecule Detection and Bead Trap** in Microstructures for Molecule Selection and DNA Degradation
Single diffusing, as well as fast flowing (10 mm/s), tetramethylrhodamine labeled molecules (TMR-4-dUTP) were detected in silicon microstructures by confocal fluorescence microscopy. Low fluorescent silicon-gl...
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Article
Characterization, Production, and Purification of Leucocin H, a Two-Peptide Bacteriocin from Leuconostoc MF215B
Leuconostoc MF215B was found to produce a two-peptide bacteriocin referred to as leucocin H. The two peptides were termed leucocin Hα and leucocin Hβ. When acting together, they inhibit, among others, Listeria m...
