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  1. No Access

    Article

    Microtubule array formation during root hair infection thread initiation and elongation in the Mesorhizobium-Lotus symbiosis

    Nuclear migration during infection thread (IT) development in root hairs is essential for legume-Rhizobium symbiosis. However, little is known about the relationships between IT formation, nuclear migration, and ...

    F. M. Perrine-Walker, M. Lartaud, H. Kouchi, R. W. Ridge in Protoplasma (2014)

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    Article

    Endoplasmic reticulum-targeted GFP reveals ER remodeling in Mesorhizobium-treated Lotus japonicus root hairs during root hair curling and infection thread formation

    The endoplasmic reticulum (ER) of the model legume Lotus japonicus was visualized using green fluorescent protein (GFP) fused with the KDEL sequence to investigate the changes ...

    F. M. Perrine-Walker, H. Kouchi, R.W. Ridge in Protoplasma (2014)

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    Article

    Nicotianamine synthase specifically expressed in root nodules of Lotus japonicus

    In dicotyledonous plants, nicotianamine synthase (NAS) is thought to play a role in the intercellular transport of iron (Fe). Fe is an essential metal for nitrogen-fixing root nodules of legumes, prompting us ...

    T. Hakoyama, H. Watanabe, J. Tomita, A. Yamamoto, S. Sato, Y. Mori, H. Kouchi in Planta (2009)

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    Article

    Regulatory regions and nuclear factors involved in nodule-enhanced expression of a soybean phosphoenolpyruvate carboxylase gene: implications for molecular evolution

    We have determined the genomic organization of two closely related phosphoenolpyruvate carboxylase genes in soybean, GmPEPC7, which is expressed at high levels in root nodules, and the housekee** gene GmPEPC15

    T. Nakagawa, K. Takane, T. Sugimoto, K. Izui, H. Kouchi in Molecular Genetics and Genomics (2003)

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    Article

    Analysis of ENOD40 expression in alb1, a symbiotic mutant of Lotus japonicus that forms empty nodules with incompletely developed nodule vascular bundles

    The alb1 mutant of Lotus japonicus (Ljsym74) forms empty nodules in which most of the bacteria remain in abnormally enlarged infection threads and fail to enter the host plant cells. The alb1 mutant was also fou...

    H. Imaizumi-Anraku, H. Kouchi, K. Syono, S. Akao in Molecular Genetics and Genomics (2000)

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    Chapter

    The Expression of Uricase Genes (NOD-35) in Ureide- and Amide-Type Legume Plants and their Molecular Evolution

    S. Tajima, M. Nomura, K. Takane, X. G. Cheng in Nitrogen Fixation: From Molecules to Crop … (2000)

  7. No Access

    Chapter

    Exploring the Genetic Potential of Rice for Forming Symbiotic Associations with Rhizobia

    P. M. Reddy, R. J. Hernandez-Oane, H. Kouchi in Nitrogen Fixation: From Molecules to Crop … (2000)

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    Article

    Endogenous protease-dependent replication of human influenza viruses in two MDCK cell lines

    Multi-cycle replication and plaque formation of influenza A and B viruses and cleavage activation of their hemagglutinin (HA) by an endogenous protease(s) were examined in two MDCK cell lines, MDCK(−) and MDCK...

    K. Noma, K. Kiyotani, H. Kouchi, Y. Fujii, Y. Egi, K. Tanaka in Archives of Virology (1998)

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    Chapter

    Expression of Two Uricase Genes and the Promoter Analysis Using Transgenic Hairy Roots

    Uricase (homotetramer of nodulin 35) is one of the major proteins in soybean nodules, and the physiological role is significant for producing ureides as major compounds to export the fixed nitrogen to the shoots.

    S. Tajima, K. Takane, H. Kouchi in Biological Nitrogen Fixation for the 21st Century (1998)

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    Chapter

    Soybean Nodule-Enhanced Phosphoenolpyruvate Carboxylase Gene Shows Striking Similarity to Another Gene for House-Kee** Isoform

    Two different cDNAs for phosphoenolpyruvate carboxylase (PEPC) were isolated from soybean root nodules. The full-lenght cDNA of the most abundant isoform, GmPEPC7, was very similar to another one, GmPEPC15, the n...

    H. Kouchi, K. Izui, S. Hata in Biological Nitrogen Fixation for the 21st Century (1998)

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    Article

    Expression pattern of tobacco cyclin genes

    We have previously isolated three cDNA clones (Ntcyc25, Ntcyc27, Ntcyc29) encoding mitotic cyclins from tobacco (Nicotiana tabacum), and in this report we describe the expression patterns of these genes. RNA gelb...

    Y. Y. Setiady, M. Sekine, T. Yamamoto, H. Kouchi, A. Shinmyo in Plant Cell Reports (1997)

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    Chapter

    Metabolism and Compartmentation of Carbon and Nitrogen in Legume Nodules

    Symbiotic association of legume plants with soil bacteria of the genera Rhizobium, Bradyrhizobium, and Azorhizobium leads to the formation of nodules in which the microsymbionts are capable of fixing atmospheric ...

    S. Tajima, H. Kouchi in Plant-microbe Interactions 2 (1997)

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    Chapter

    Signal Exchange During Soybean Nodulation by Bradyrhizobium Japonicum

    Rhizobium, Azorhizobium and Bradyrhizobium species infect leguminous plants and induce root organogenesis resulting in a nodule in which the symbionts reside. Legume nodules can be classified int...

    G. Stacey, E. Minami, H. Kouchi, J. R. Cohn in Nitrogen Fixation: Fundamentals and Applic… (1995)