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Article
Cloning and functional analysis of EpGHQH1 in shikonin production of Echium plantagineum
Shikonins are the main naphthoquinones in the root periderm of Echium plantagineum, as well as the hypocotyl and root hairs of young seedlings. These red pigments can be used as dyes or for medicinal uses since a...
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Article
Knockouts of a late flowering gene via CRISPR–Cas9 confer early maturity in rice at multiple field locations
OsGhd7 gene was discovered by screening our rice activation tagging population. CRISPR–Cas9 created knockouts of OsGhd7 conferred early flowering and early maturity in rice varieties across multiple geographical ...
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Article
A cytokinin-activation enzyme-like gene improves grain yield under various field conditions in rice
CRISPR-edited variants at the 3′-end of OsLOGL5's coding sequence (CDS), significantly increased rice grain yield under well-watered, drought, normal nitrogen, and low nitrogen field conditions at multiple geogra...
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Article
Transgenic analysis reveals LeACS-1 as a positive regulator of ethylene-induced shikonin biosynthesis in Lithospermum erythrorhizon hairy roots
The phytohormone ethylene (ET) is a crucial signaling molecule that induces the biosynthesis of shikonin and its derivatives in Lithospermum erythrorhizon shoot cultures. However, the molecular mechanism and the ...
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Article
The Promoters of Two Isoflavone Synthase Genes Respond Differentially to Nodulation and Defense Signals in Transgenic Soybean Roots
Isoflavonoids are a group of secondary metabolites common to leguminous plants that play roles in nodulation and defense responses. Isoflavone synthase (IFS) catalyzes the key entry point step of isoflavone bi...
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Article
A single Arabidopsis GF14 isoform possesses biochemical characteristics of diverse 14-3-3 homologues
Arabidopsis cDNA clones of GF14 proteins originally were isolated on the basis of their association with the G-box DNA/protein complex by a monoclonal antibody screening approach. GF14 proteins are homologous to...
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Article
Site-specific oligodeoxynucleotide binding to maize Adh1 gene promoter represses Adh1-GUS gene expression in vivo
There is a 36 bp tract of extreme homopurine/homopyrimidine (PuPy) asymmetry in the maize Adh1 gene promoter (from −44 to −79) that is S1-hypersensitive in plasmids under supercoil tension. Oligodeoxynucleotides ...