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Article
MiR-146a induces the aging of mesenchymal stem cell via CASK
Mesenchymal stem cell (MSC) has elicited great hopes for regenerative medicine, however, its regenerative potential decreases with age, which raises questions about the efficiency of autologous transplantation...
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Article
Open AccessMorphology-based deep learning enables accurate detection of senescence in mesenchymal stem cell cultures
Cell senescence is a sign of aging and plays a significant role in the pathogenesis of age-related disorders. For cell therapy, senescence may compromise the quality and efficacy of cells, posing potential saf...
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Article
Continuous estimation of multi-DOF movement from sEMG based on non-negative matrix factorization and L2 regulation
Accurate continuous estimation of multi-DOF movement is crucial for simultaneous control of advanced myoelectric prosthetic. The decoupling of multi-DOF is a challenge for continuous estimation. In this paper,...
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Article
Development of the T-ALLiPSC-based therapeutic cancer vaccines for T-cell acute lymphoblastic leukemia
The T-cell acute lymphoblastic leukemia (T-ALL) is a kind of hematological malignancy in children. Despite the significant improvement in the cure rate of T-ALL upon treatment with chemotherapy regimens, stero...
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Protocol
Generation of Monoclonal iPSC Lines with Stable Cas9 Expression and High Cas9 Activity
CRISPR/Cas9-mediated gene editing has been rapidly and widely applied in many organisms for delicate genetic manipulation, including human-induced pluripotent stem cells (iPSCs). Gene editing in human iPSCs is...
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Protocol
CRISPR Guide RNA Library Screens in Human Induced Pluripotent Stem Cells
High-throughput CRISPR guide RNA (gRNA) library screen, that is, CRISPR/Cas9 screen, enables the unbiased identification of gene functions in a variety of biological processes. Typical pooled CRISPR/Cas9 scree...
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Chapter and Conference Paper
Semi-supervised Yolo Network for Induced Pluripotent Stem Cells Detection
Induced pluripotent stem cells (iPSCs) have broad prospects in clinical and industrial applications, and the automatic identification of iPSCs is essential to optimize the iPSCs manufacturing. However, the cha...
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Article
Open AccessIntra-articular delivery of extracellular vesicles secreted by chondrogenic progenitor cells from MRL/MpJ superhealer mice enhances articular cartilage repair in a mouse injury model
Chondrogenic progenitor cells (CPCs) have high self-renewal capacity and chondrogenic potential. Intra-articular delivery of purified mesenchymal stem cells (MSCs) from MRL/MpJ “superhealer” mice increased bon...
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Article
Oral Administration of Strontium Gluconate Effectively Reduces Articular Cartilage Degeneration Through Enhanced Anabolic Activity of Chondrocytes and Chondrogenetic Differentiation of Mesenchymal Stromal Cells
Osteoarthritis (OA), a common degenerative disease affecting articular cartilage, is caused by multiple factors, and currently, there are few approaches to effectively delay its progression. This study aimed t...
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Article
Open AccessRepair of cartilage defects in osteoarthritis rats with induced pluripotent stem cell derived chondrocytes
The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA)...
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Article
Open AccessMesenchymal stem cells derived from human induced pluripotent stem cells retain adequate osteogenicity and chondrogenicity but less adipogenicity
Previously, we established a simple method for deriving mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSC-MSCs). These iPSC-MSCs were capable of forming osteogenic structures in sca...
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Article
Open AccessHIF-1-modified BMSCs improve migration and reduce neuronal apoptosis after stroke in rats
Bone marrow-derived mesenchymal stem cells (BMSCs) have been demonstrated ameliorating neurologic deficits after stroke. Hypoxia-inducible factor-1 (HIF-1), the key regulator of cellular responses to low oxygen c...
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Article
Open AccessCis-regulatory functions of overlap** HIF-1alpha/E-box/AP-1-like sequences of CD164
CD164 (also known as MGC-24v or endolyn) is a sialomucin which has been suggested to participate in regulating the proliferation, cell adhesion and differentiation of hematopoietic stem and progenitor cells. C...
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Article
Open AccessInduction-dependent neural marker expression and electrophysiological characteristics of bone marrow mesenchymal stem cells that naturally express high levels of nestin
Under certain experimental conditions, bone marrow mesenchymal stem cells (MSCs) express neuronal phenotypes and neuronal markers, which suggests that they could be used to treat various neurological diseases....
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Article
Apoptosis and p53 gene expression in male reproductive tissues of cadmium exposed rats
Reverse transcription (RT) PCR technique was used to investigate the mechanism of apoptosis induced by Cd and the change of its related genes in testes and prostate of rats. Adult male rats were given a single...
