Rab GTPases
Methods and Protocols
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13 Result(s)
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GuangPu **e
Biochemistry, general
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Protocol
Newer Methods Drive Recent Insights into Rab GTPase Biology: An Overview
The conserved Ypt/Rab GTPases regulate all major intracellular protein traffic pathways, including secretion, endocytosis and autophagy. These GTPases undergo distinct changes in conformation between their GTP...
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Protocol
Use of Immunohistochemistry to Determine Expression of Rab5 Subfamily of GTPases in Mature and Developmental Brains
Rab GTPases are essentially molecular switches. They serve as master regulators in intracellular membrane trafficking from the formation and transport of vesicles at the originating organelle to its fusion to ...
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Book
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Article
ESCRT-III accessory proteins regulate fungal development and plant infection in Fusarium graminearum
Ubiquitinated biosynthetic and surface proteins destined for degradation are sorted into the lysosome/vacuole via the multivesicular body sorting pathway, which depends on the function of ESCRT machinery. Fusa...
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Article
Multiprotein-bridging factor 1 regulates vegetative growth, osmotic stress, and virulence in Magnaporthe oryzae
Multiprotein bridging factor 1 (MBF1) is a transcriptional co-activator that mediates transcriptional activation by bridging sequence-specific activator like proteins and the TATA-box binding protein (TBP). MB...
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Book
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Protocol
Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells with or without NGF
Macroautophagy selectively recycles damaged or unneeded proteins and organelles by degradation via targeting to the autophagosome. The following method seeks to identify candidate Rab GTPases that likely modul...
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Protocol
Determination of Rab5 Activity in the Cell by Effector Pull-Down Assay
Rab5 targets to early endosomes and is a master regulator of early endosome fusion and endocytosis in all eukaryotic cells. Like other GTPases, Rab5 functions as a molecular switch by alternating between GTP-b...
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Protocol
Rab Family of GTPases
Rab proteins represent the largest branch of the Ras-like small GTPase superfamily and there are 66 Rab genes in the human genome. They alternate between GTP- and GDP-bound states, which are facilitated by gua...
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Chapter
Endocytosis and the Regulation of Cell Signaling, Cell Adhesion, and Epithelial to Mesenchymal Transition in Cancer
Endosomes play key roles in the control and execution of such diverse and spatially restricted processes as cell signaling, epithelial to mesenchymal transitions as well as cell adhesion and migration. The end...
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Article
Structural basis of Rab5-Rabaptin5 interaction in endocytosis
Rab5 is a small GTPase that regulates early endosome fusion. We present here the crystal structure of the Rab5 GTPase domain in complex with a GTP analog and the C-terminal domain of effector Rabaptin5. The pr...
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Article
Sensitivity and specificity amplification in signal transduction
Intracellular signal transduction pathways transmit signals from the cell surface to various intracellular destinations, such as cytoskeleton and nucleus through a cascade of protein-protein interactions and a...
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Chapter and Conference Paper
GTPases: Key regulatory components of the endocytic pathway
GTP-binding proteins or GTPases are versatile cyclic molecular switches (Gilman, A.G. 1987 ; Bourne et al., 1990, 1992). In the past few years there has been an explosion of interest in unraveling the role of ...
