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    Article

    Genome-Wide Editing Provides Insights into Role of Unsaturated fatty Acids in Low Temperature Growth of the Psychrotrophic Yeast Metschnikowia bicuspidata var. australis W7-5

    In order to know the function of C18:2 and C18:3 fatty acids in the cold growth of the psychrotrophic yeast M. bicuspidata var. australis W7-5, the mutant 1 without C18:2 fatty acid and the mutant 2 without C18:3

    **n Wei, Miao Zhang, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi in Marine Biotechnology (2023)

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    Production, Gene Cloning, and Overexpression of a Laccase in the Marine-Derived Yeast Aureobasidium melanogenum Strain 11-1 and Characterization of the Recombinant Laccase

    Aureobasidium melanogenum strain 11-1 with a high laccase activity was isolated from a mangrove ecosystem. Under the optimal conditions, the 11-1 strain yielded the highest laccase activity up to 3120.0 ± 170 mU/...

    Thu Aung, Hong Jiang, Cheng-Cheng Chen, Guang-Lei Liu, Zhong Hu in Marine Biotechnology (2019)

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    Production, Purification, and Gene Cloning of a β-Fructofuranosidase with a High Inulin-hydrolyzing Activity Produced by a Novel Yeast Aureobasidium sp. P6 Isolated from a Mangrove Ecosystem

    After screening of over 300 yeast strains isolated from the mangrove ecosystems, it was found that Aureobasidium sp. P6 strain had the highest inulin-hydrolyzing activity. Under the optimal conditions, this yeast...

    Hong Jiang, Yan Ma, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi in Marine Biotechnology (2016)

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    Cloning and Characterization of a Pyruvate Carboxylase Gene from Penicillium rubens and Overexpression of the Genein the Yeast Yarrowia lipolytica for Enhanced Citric Acid Production

    In this study, a pyruvate carboxylase gene (PYC1) from a marine fungus Penicillium rubens I607 was cloned and characterized. ORF of the gene (accession number: KM397349.1) had 3534 bp encoding 1177 amino acids wi...

    Ge-Yi Fu, Yi Lu, Zhe Chi, Guang-Lei Liu, Shou-Feng Zhao, Hong Jiang in Marine Biotechnology (2016)

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    Genetic Modification of the Marine-Isolated Yeast Aureobasidium melanogenum P16 for Efficient Pullulan Production from Inulin

    In this study, in order to directly and efficiently convert inulin into pullulan, the INU1 gene from Kluyveromyces maximum KM was integrated into the genomic DNA and actively expressed in the high pullulan produc...

    Zai-Chao Ma, Nan-Nan Liu, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi in Marine Biotechnology (2015)

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    Both Decrease in ACL1 Gene Expression and Increase in ICL1 Gene Expression in Marine-Derived Yeast Yarrowia lipolytica Expressing INU1 Gene Enhance Citric Acid Production from Inulin

    In this study, some of the ATP-citrate lyase genes (ACL1) were deleted and the copy number of the iso-citrate lyase gene (ICL1) was increased in the marine-derived yeast Yarrowia lipolytica SWJ-1b displaying the ...

    **ao-Yan Liu, Zhe Chi, Guang-Lei Liu, Catherine Madzak in Marine Biotechnology (2013)

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    Article

    Cloning of Exo-β-1,3-glucanase Gene from a Marine Yeast Williopsis saturnus and Its Overexpression in Yarrowia lipolytica

    The exo-β-1,3-glucanase structural gene (WsEXG1 gene, accession number: FJ875997.2) was isolated from both the genomic DNA and cDNA of the marine yeast Williopsis saturnus WC91-2 by inverse PCR and RT-PCR. An ope...

    Ying Peng, Guang-Lei Liu, **n-Jun Yu, **ang-Hong Wang, Li **g in Marine Biotechnology (2011)