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Article
Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification
Current imaging approaches limit the ability to perform multi-scale characterization of three-dimensional (3D) organotypic cultures (organoids) in large numbers. Here, we present an automated multi-scale 3D im...
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Protocol
Artificial Microniche Array with Spatially Structured Biochemical Cues
We present here a method to create arrays of microcavities that can be differentially coated on their bottom, side, and top with different proteins. These cavities range in size from single cell to multicellul...
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Article
Expansion of patient-derived circulating tumor cells from liquid biopsies using a CTC microfluidic culture device
This protocol describes a microfluidics approach for culturing liquid-biopsy-derived circulating tumor cell clusters to predict a patient's response toward various therapeutic strategies.
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Article
3D high- and super-resolution imaging using single-objective SPIM
Light-sheet microscopy using a single objective and micromirrors to orient the light sheet enables high- and super-resolution imaging of cells and embryos on a standard microscope.