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Article
Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification
Current imaging approaches limit the ability to perform multi-scale characterization of three-dimensional (3D) organotypic cultures (organoids) in large numbers. Here, we present an automated multi-scale 3D im...
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Article
Open AccessCirculating Tumor Cell cluster phenotype allows monitoring response to treatment and predicts survival
Circulating tumor cells (CTCs) are putative markers of tumor prognosis and may serve to evaluate patient’s response to chemotherapy. CTCs are often detected as single cells but infrequently as clusters and are...
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Article
Open AccessLow-dose anti-inflammatory combinatorial therapy reduced cancer stem cell formation in patient-derived preclinical models for tumour relapse prevention
Emergence of drug-resistant cancer phenotypes is a challenge for anti-cancer therapy. Cancer stem cells are identified as one of the ways by which chemoresistance develops.
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Protocol
Artificial Microniche Array with Spatially Structured Biochemical Cues
We present here a method to create arrays of microcavities that can be differentially coated on their bottom, side, and top with different proteins. These cavities range in size from single cell to multicellul...
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Article
Expansion of patient-derived circulating tumor cells from liquid biopsies using a CTC microfluidic culture device
This protocol describes a microfluidics approach for culturing liquid-biopsy-derived circulating tumor cell clusters to predict a patient's response toward various therapeutic strategies.
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Article
Chemical vapour deposition of zeolitic imidazolate framework thin films
Integrating metal–organic frameworks (MOFs) in microelectronics has disruptive potential because of the unique properties of these microporous crystalline materials. Suitable film deposition methods are crucia...
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Article
3D high- and super-resolution imaging using single-objective SPIM
Light-sheet microscopy using a single objective and micromirrors to orient the light sheet enables high- and super-resolution imaging of cells and embryos on a standard microscope.
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Article
Continuous adsorption in highly ordered porous matrices made by nanolithography
The exposed surface area of porous materials is usually determined by measuring the mass of adsorbed gas as a function of vapour pressure. Here we report a comprehensive study of adsorption in systems with clo...
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Article
Positive resist for UV and X-ray lithography synthesized through sol–gel chemistry
A positive tone resist for UV and X-ray lithography synthesized starting from an organically modified silicon alkoxide, bis(triethoxysilyl)benzene, through the sol–gel method, either in basic or in acid cataly...