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  1. No Access

    Article

    Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification

    Current imaging approaches limit the ability to perform multi-scale characterization of three-dimensional (3D) organotypic cultures (organoids) in large numbers. Here, we present an automated multi-scale 3D im...

    Anne Beghin, Gianluca Grenci, Geetika Sahni, Su Guo, Harini Rajendiran in Nature Methods (2022)

  2. Article

    Open Access

    Circulating Tumor Cell cluster phenotype allows monitoring response to treatment and predicts survival

    Circulating tumor cells (CTCs) are putative markers of tumor prognosis and may serve to evaluate patient’s response to chemotherapy. CTCs are often detected as single cells but infrequently as clusters and are...

    Ajay Balakrishnan, Deepak Koppaka, Abhishek Anand, Barnali Deb in Scientific Reports (2019)

  3. Article

    Open Access

    Low-dose anti-inflammatory combinatorial therapy reduced cancer stem cell formation in patient-derived preclinical models for tumour relapse prevention

    Emergence of drug-resistant cancer phenotypes is a challenge for anti-cancer therapy. Cancer stem cells are identified as one of the ways by which chemoresistance develops.

    Bee Luan Khoo, Gianluca Grenci, Joey Sze Yun Lim, Yan ** Lim in British Journal of Cancer (2019)

  4. No Access

    Protocol

    Artificial Microniche Array with Spatially Structured Biochemical Cues

    We present here a method to create arrays of microcavities that can be differentially coated on their bottom, side, and top with different proteins. These cavities range in size from single cell to multicellul...

    Xumei Gao, Celine Stoecklin, Yue Zhang, Zhe Weng, Richard De Mets in Cell-Based Microarrays (2018)

  5. No Access

    Article

    Expansion of patient-derived circulating tumor cells from liquid biopsies using a CTC microfluidic culture device

    This protocol describes a microfluidics approach for culturing liquid-biopsy-derived circulating tumor cell clusters to predict a patient's response toward various therapeutic strategies.

    Bee Luan Khoo, Gianluca Grenci, Ying Bena Lim, Soo Chin Lee in Nature Protocols (2018)

  6. No Access

    Article

    Chemical vapour deposition of zeolitic imidazolate framework thin films

    Integrating metal–organic frameworks (MOFs) in microelectronics has disruptive potential because of the unique properties of these microporous crystalline materials. Suitable film deposition methods are crucia...

    Ivo Stassen, Mark Styles, Gianluca Grenci, Hans Van Gorp in Nature Materials (2016)

  7. No Access

    Article

    3D high- and super-resolution imaging using single-objective SPIM

    Light-sheet microscopy using a single objective and micromirrors to orient the light sheet enables high- and super-resolution imaging of cells and embryos on a standard microscope.

    Remi Galland, Gianluca Grenci, Ajay Aravind, Virgile Viasnoff in Nature Methods (2015)

  8. No Access

    Article

    Continuous adsorption in highly ordered porous matrices made by nanolithography

    The exposed surface area of porous materials is usually determined by measuring the mass of adsorbed gas as a function of vapour pressure. Here we report a comprehensive study of adsorption in systems with clo...

    Giampaolo Mistura, Alessandro Pozzato, Gianluca Grenci in Nature Communications (2013)

  9. No Access

    Article

    Positive resist for UV and X-ray lithography synthesized through sol–gel chemistry

    A positive tone resist for UV and X-ray lithography synthesized starting from an organically modified silicon alkoxide, bis(triethoxysilyl)benzene, through the sol–gel method, either in basic or in acid cataly...

    Laura Brigo, Gianluca Grenci in Journal of Sol-Gel Science and Technology (2011)