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Article
Open AccessMicroridge-like structures anchor motile cilia
Several tissues contain cells with multiple motile cilia that generate a fluid or particle flow to support development and organ functions; defective motility causes human disease. Developmental cues orient mo...
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Article
Open AccessPhosphoproteomics identifies dual-site phosphorylation in an extended basophilic motif regulating FILIP1-mediated degradation of filamin-C
The PI3K/Akt pathway promotes skeletal muscle growth and myogenic differentiation. Although its importance in skeletal muscle biology is well documented, many of its substrates remain to be identified. We here...
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Article
Open AccessOptogenetic control of integrin-matrix interaction
Optogenetic approaches have gathered momentum in precisely modulating and interrogating cellular signalling and gene expression. The use of optogenetics on the outer cell surface to interrogate how cells recei...
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Protocol
Light-Regulated Protein Kinases Based on the CRY2-CIB1 System
Optogenetic approaches enable the control of biological processes in a time- and space-resolved manner. These light-based methods are noninvasive and by using light as sole activator minimize side effects in c...
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Article
Open AccessOptogenetic clustering of CNK1 reveals mechanistic insights in RAF and AKT signalling controlling cell fate decisions
Scaffold proteins such as the multidomain protein CNK1 orchestrate the signalling network by integrating and controlling the underlying pathways. Using an optogenetic approach to stimulate CNK1 uncoupled from ...
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Article
Open AccessOptogenetically controlled RAF to characterize BRAF and CRAF protein kinase inhibitors
Here, we applied optoRAF, an optogenetic tool for light-controlled clustering and activation of RAF proteins that mimics the natural occurring RAS-mediated dimerization. This versatile tool allows studying the...
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Article
Open AccessPharmacologically Controlled Protein Switch for ON-OFF Regulation of Growth Factor Activity
The precise manipulation of growth factor signaling is central to the progress of tissue engineering. Methods for direct time-resolved activation of signaling pathways through controlled receptor dimerization ...
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Article
Phosphorylierungen bei Signalproteinen erkennen und deuten
Detailed knowledge of phosphorylation events in proteins is an important key for a better understanding of biological signalling processes in health and disease. The combined use of traditional biochemical met...
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Article
Retroviral gene transfer of dominant negative Raf-1 mutants suppresses Ha-ras-induced transformation and delays tumor formation
Activating mutants of ras are among the most frequently found genetic alterations in human cancers. Therefore, Ras appears to be an attractive target for therapeutic intervention using gene transfer. The protein ...
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Article
MEK1 mediates a positive feedback on Raf-1 activity independently of Ras and Src
Growth factor stimulated receptor tyrosine kinases activate a protein kinase cascade via the serine/threonine protein kinase Raf-1. Direct upstream activators of Raf-1 are Ras and Src. This study shows that ME...
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Article
Complementation analysis of mutants defective in different steps of HBV reverse transcription
The HBV P gene encodes a multifuctional polyprotein which contains the major enzymatic activities required for hepadnaviral reverse transcription (protein primer, DNA polymerase, and RNase H). Mutant HBV genom...
