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Article
Expression of the Legume-Specific Nod Factor Receptor Proteins Alters Developmental and Immune Responses in Rice
Legumes form symbiosis with rhizobia, which fix nitrogen for the benefit of host plant in return for carbon resources. Development of this unique symbiosis in legumes is triggered by rhizobia-secreted nodulati...
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Article
Identification of miRNAs linked to peanut nodule functional processes
microRNAs (miRNAs) are non-coding small RNAs that regulate gene expression at post-transcriptional level. Thousands of miRNAs have been identified in legumes, but studies about miRNAs linked to peanut nodule f...
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Protocol
Functional Analysis of Root microRNAs by a Constitutive Overexpression Approach in a Composite Plant System
Agrobacterium-mediated transformation is a fast and efficient method for genome modification in plants. In this protocol, we apply this technique for the analysis of root microRNA functionality. The induction of ...
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Article
Expression in rice of an autoactive variant of Medicago truncatula DMI3, the Ca+2/calmodulin-dependent protein kinase from the common symbiotic pathway modifies root transcriptome and improves mycorrhizal colonization
Rice is the principle staple food for more than half of humankind. Frequently, productivity of rice is affected by low nitrogen in the soil and hence, for enhanced rice production it heavily relies on syntheti...
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Article
Open AccessIdentification and analysis of alternative splicing events in Phaseolus vulgaris and Glycine max
The vast diversification of proteins in eukaryotic cells has been related with multiple transcript isoforms from a single gene that result in alternative splicing (AS) of primary transcripts. Analysis of RNA s...
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Article
Rhizobium etli bacteroids engineered for Vitreoscilla hemoglobin expression alleviate oxidative stress in common bean nodules that reprogramme global gene expression
The common bean (Phaseolus vulgaris L.)–Rhizobium etli symbiosis and crop productivity are highly affected by adverse environmental conditions that cause oxidative stress. Based on the improved symbiosis of commo...
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Article
Open AccessGenome-wide identification of the Phaseolus vulgaris sRNAome using small RNA and degradome sequencing
MiRNAs and phasiRNAs are negative regulators of gene expression. These small RNAs have been extensively studied in plant model species but only 10 mature microRNAs are present in miRBase version 21, the most u...
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Article
Open AccessCommon bean (Phaseolus vulgarisL.) PvTIFY orchestrates global changes in transcript profile response to jasmonate and phosphorus deficiency
TIFY is a large plant-specific transcription factor gene family. A subgroup of TIFY genes named JAZ (Jasmonate-ZIM domain) has been identified as repressors of jasmonate (JA)-regulated transcription in Arabidopsi...
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Article
Open AccessTranscript profiling of common bean (Phaseolus vulgaris L.) using the GeneChip®Soybean Genome Array: optimizing analysis by masking biased probes
Common bean (Phaseolus vulgaris L.) and soybean (Glycine max) both belong to the Phaseoleae tribe and share significant coding sequence homology. This suggests that the GeneChip® Soybean Genome Array (soybean Gen...
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Article
Open AccessMetabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system
Shikimic acid (SA) is utilized in the synthesis of oseltamivir-phosphate, an anti-influenza drug. In this work, metabolic engineering approaches were employed to produce SA in Escherichia coli strains derived fro...
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Article
Regeneration of different cultivars of common bean (Phaseolus vulgaris L.) via indirect organogenesis
A protocol for in vitro regeneration via indirect organogenesis for Phaseolus vulgaris cv. Negro Jamapa was established. The explants used were apical meristems and cotyledonary nodes dissected from the embryonic...
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Article
Open AccessCoutilization of glucose and glycerol enhances the production of aromatic compounds in an Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system
Escherichia coli strains lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system (PTS) are capable of coutilizing glucose and other carbon sources due to the absence of catabolite repression by gl...
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Chapter
Modulation of Ammonium Assimilation in Transgenic Legumes During the Symbiosis with Rhizobium
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Book
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Chapter
Modulation of Key Symbiotic Metabolic Pathways Through Reverse Genetics of Transgenic Legumes
The use of reverse genetics in plants offers the possibility to modulate the expression of specific genes important for certain metabolic pathway and therefore to vary quantitatively the flux control of the pr...
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Article
Establishment of transformed root cultures of Perezia cuernavacana producing the sesquiterpene quinone perezone
The sesquiterpene quinone currently known as perezone is abundantly produced by the roots of Perezia cuernavacana. This compound is of biotechnological interest since it may be used as a pigment and has several p...
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Article
Root exuded nod-gene inducing signals limit the nodulation capacity of different alfalfa varieties with Rhizobium meliloti
Different nodulation capacities were found among nine different varieties of alfalfa, cultivated in the Central region of Mexico, by Rhizobium meliloti 2011. A correlation between nodulation capacity and foliar d...
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Article
Embryogenic response of Mexican alfalfa (Medicago sativa) varieties
The in vitro embryogenic response of nine varieties of alfalfa (Medicago sativa L.) grown in México (five Mexican varieties: Puebla 76, Inia 76, Bajío 76, Sintético I and Sintético II and four foreign or introduc...
