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Article
Open AccessEnhanced energy savings in enzymatic refining of hardwood and softwood pulp
Pulp refining is an energy consuming, but integral part of paper production with the aim to increase tensile strength and smoothness of paper sheets. Commercial enzyme formulations are used to lower the energy...
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Article
Open AccessMechanistic investigation of the effect of endoglucanases related to pulp refining
Endoglucanases are increasingly being touted as the ultimate solution for reducing energy consumption during the refining process in the pulp and paper industry. However, due to the high variety of endoglucana...
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Delivery of Biomolecules Using Chitosan Wound Dressings
The role of wound dressings has significantly transformed from by providing a simple protective function to dressings that actively participate in every stage of the wound healing process. Chitosan is one of t...
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Article
Open AccessAuthor Correction: Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessShotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos
Knufia chersonesos is an ascomycotal representative of black fungi, a morphological group of polyextremotolerant melanotic fungi, whose ability to resort to recalcitrant carbon sources makes it an interesting can...
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Article
Open AccessEnzymatic synthesis of lignin derivable pyridine based polyesters for the substitution of petroleum derived plastics
Following concerns over increasing global plastic pollution, interest in the production and characterization of bio-based and biodegradable alternatives is rising. In the present work, the synthesis of a serie...
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Article
Surface engineering of polyester-degrading enzymes to improve efficiency and tune specificity
Certain members of the carboxylesterase superfamily can act at the interface between water and water-insoluble substrates. However, nonnatural bulky polyesters usually are not efficiently hydrolyzed. In the re...
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Article
Open AccessSynergistic effect of mutagenesis and truncation to improve a polyesterase from Clostridium botulinum for polyester hydrolysis
The activity of the esterase (Cbotu_EstA) from Clostridium botulinum on the polyester poly(ethylene terephthalate) (PET) was improved by concomitant engineering of two different domains. On the one hand, the zinc...
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Article
Open AccessPpEst is a novel PBAT degrading polyesterase identified by proteomic screening of Pseudomonas pseudoalcaligenes
A novel esterase, PpEst, that hydrolyses the co-aromatic-aliphatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT) was identified by proteomic screening of the Pseudomonas pseudoalcaligenes secretome...
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Article
Characterization of a poly(butylene adipate-co-terephthalate)-hydrolyzing lipase from Pelosinus fermentans
Certain α/β hydrolases have the ability to hydrolyze synthetic polyesters. While their partial hydrolysis has a potential for surface functionalization, complete hydrolysis allows recycling of valuable buildin...
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Article
Biomarkers for infection: enzymes, microbes, and metabolites
Wound infection is a severe complication causing delayed healing and risks for patients. Conventional methods of diagnosis for infection involve error-prone clinical description of the wound and time-consuming...
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Microbiology and Molecular Biology Tools for Biogas Process Analysis, Diagnosis and Control
Many biotechnological processes such as biogas production or defined biotransformations are carried out by microorganisms or tightly cooperating microbial communities. Process breakdown is the maximum credible...
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Article
Microbial Conversion of Crude Glycerol to Dihydroxyacetone
Glycerol is increasingly being used as a versatile raw material within the biorefinery. However, it is very expensive to refine the crude glycerol to a high purity. In this study, the biotransformation of crud...
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Article
Open AccessSemi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production
The ability of fungal cellobiose dehydrogenase (CDH) to generate H2O2in-situ is highly interesting for biotechnological applications like cotton bleaching, laundry detergents or antimicrobial functionalization of...
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Article
Enzyme-based online monitoring and measurement of antioxidant activity using an optical oxygen sensor coupled to an HPLC system
It is estimated that up to 50 % of the adult population take antioxidant products on a daily basis to promote their health status. Strangely, despite the well-recognized importance of antioxidants, currently t...
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Article
Protein disulphide isomerase-assisted functionalization of keratin-based matrices
In living systems, protein disulphide isomerase (PDI, EC 5.3.4.1) regulates the formation of new disulphide bonds in proteins (oxidase activity) and catalyzes the rearrangement of non-native disulphide bonds (...
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Chapter
Hydrolases in Polymer Chemistry: Part III: Synthesis and Limited Surface Hydrolysis of Polyesters and Other Polymers
Limited enzymatic surface hydrolysis of polyamides, polyethyleneterphthalates (PET) and polyacrylonitriles has been demonstrated to be a powerful and yet mild strategy for directly improving polymer surface pr...
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Article
Enzymatic reduction of complex redox dyes using NADH-dependent reductase from Bacillus subtilis coupled with cofactor regeneration
Conventional vat dyeing involves chemical reduction of dyes into their water-soluble leuco form generating considerable amounts of toxic chemicals in effluents. In the present study, a new β-nicotinamide adeni...
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Article
Antioxidant activity assay based on laccase-generated radicals
A novel antioxidant activity assay was developed using laccase-oxidized phenolics. In a three-step approach, phenolic compounds were first oxidized by laccase. Laccase was then inhibited using 80% (v/v) methan...
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Article
Siloxane removal from biogas by biofiltration: biodegradation studies
Recently a lot of attention has been focused on volatile methyl siloxanes (VMSs) in biogas because of the costly problems deriving from the formation of silicate-based deposits in biogas-fuelled power plant eq...
