43 Result(s)

Article

Expression of a synthetic cholera toxin B subunit in tobacco using ubiquitin promoter and bar gene as a selectable marker

A protocol has been developed to produce a cholera toxin B subunit (CTB) in tobacco tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. The synthetic CTB subunit gene was altered t...

Tae-** Kang, Bang-Geul Kim, Ji-Yeon Yang, Moon-Sik Yang in Molecular Biotechnology (2006)

Article

Expression of a synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat labile enterotoxin in rice endosperm

Epitopes often require co-delivery with adjuvant and targeting proteins to enable recognition by the immune system, and this approach may also increase the efficacy of the antigen. In this study, we assess and...

Maria Oszvald, Tae-** Kang, Sandor Tomoskozi, Cecilia Tamas… in Molecular Biotechnology (2007)

Article

A Novel Strategy for Analyzing RNA-Protein Interactions by Surface Plasmon Resonance Biosensor

Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on...

Yaling Yang, Quan Wang, Deyin Guo in Molecular Biotechnology (2008)

Article

A Bipartite Recombinant Yeast System for the Identification of Subtype-Selective Estrogen Receptor Ligands

Kaiwei Liang, Liuqing Yang, Zhimin **ao, Jian Huang in Molecular Biotechnology (2009)

Article

Correction: Corrigendum: Identifying polyglutamine protein species in situ that best predict neurodegeneration

Nat. Chem. Biol. 7, 925–934 (2011); published online 30 October 2011; corrected after print 2 December 2011 In the version of this article initially published, lanes 6 and 8 in Figure 6b had identical blot ima...

Jason Miller, Montserrat Arrasate, Elizabeth Brooks… in Nature Chemical Biology (2012)

Article

Correction: Corrigendum: N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO

Nat. Chem. Biol. 7, 885–887 (2011); published online 16 October 2011; corrected after print 3 July 2012 In the version of this article initially published, one of the institute affiliations of co–first author ...

Guifang Jia, Ye Fu, Xu Zhao, Qing Dai, Guanqun Zheng, Ying Yang… in Nature Chemical Biology (2012)

Article

Erratum: Pyruvate kinase M2 activators promote tetramer formation and suppress tumorigenesis

Nat. Chem. Biol. 8, 839–847 (2012); published online 26 August 2012; corrected after print 11 October 2012 In the version of this article initially published, the accession codes to the Protein Data Bank were ...

Dimitrios Anastasiou, Yimin Yu, William J Israelsen… in Nature Chemical Biology (2012)

Chapter

Application of Molecular Beacons in Real-Time PCR

Real-time PCR or quantitative PCR (QPCR) is a powerful technique that allows measurement of PCR product while the amplification reaction proceeds. It incorporates the fluorescent element into conventional PCR ...

Chunming Wang, Chaoyong James Yang in Molecular Beacons (2013)

Article

Construction of an Immunostimulatory Plasmid, pUCpGs10, and Research on its Immune Adjuvant Effect

In order to overcome the instability of CpG ODN in vivo, sequence diversity, and individual differences, eleven CpG ODN fragments were meticulously selected and linked to form a Multi-CpG, which were repeatedl...

Li Tang, **aoyan Feng, Feng He, Rui Huang, **g He, Bingshui **u… in Molecular Biotechnology (2013)

Article

Correction: Corrigendum: Regulation of nuclear PKA revealed by spatiotemporal manipulation of cyclic AMP

Nat. Chem. Biol. 8, 375–382 (2012); published online 26 February 2012; corrected after print 19 April 2013 In the version of this article initially published, the domain schemes in Figure 1a were incorrectly l...

Vedangi Sample, Lisa M DiPilato, Jason H Yang, Qiang Ni… in Nature Chemical Biology (2013)

Article

M Cell-Targeting Ligand and Consensus Dengue Virus Envelope Protein Domain III Fusion Protein Production in Transgenic Rice Calli

The spread of dengue (DEN) virus is becoming a major concern due to the possibility of primary infection with one of the four dengue serotypes (DEN 1–4) and secondary infection with other heterotypes, which ca...

Tae-Geum Kim, Mi-Young Kim, Nguyen-Xuan Huy, Sae-Hae Kim… in Molecular Biotechnology (2013)

Article

Dengue Virus E Glycoprotein Production in Transgenic Rice Callus

Dengue is a disease caused by dengue virus and represents the most important arthropod-borne viral disease in humans. Dengue virus enters host cells via binding of envelope glycoprotein (E) to a receptor. In t...

Tae-Geum Kim, Mi-Young Kim, Nguyen-Quang-Duc Tien… in Molecular Biotechnology (2014)

Article

Correction: Corrigendum: Sterol metabolism controls T_H17 differentiation by generating endogenous RORγ agonists

Nat. Chem. Biol. 11, 141–147 (2015); published online 5 January 2015; corrected after print 15 July 2015 In the version of this article initially published, the zymosterol and zymostenol structures shown in Fi...

**ao Hu, Yahong Wang, Ling-Yang Hao, **kui Liu, Chuck A Lesch… in Nature Chemical Biology (2015)

Article

Publisher Correction: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

In the version of this article originally published, the bottom of Figure 4f,g was partially truncated in the PDF. The error has been corrected in the PDF version of this article.

Kiryl D. Piatkevich, Erica E. Jung, Christoph Straub… in Nature Chemical Biology (2018)

Article

Publisher Correction: The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases

In the version of this article initially published, authors Sarah E. Wilkins, Charlotte D. Eaton, Martine I. Abboud and Maximiliano J. Katz were incorrectly included in the equal contributions footnote in the ...

Suzana Markolovic, Qinqin Zhuang, Sarah E. Wilkins… in Nature Chemical Biology (2018)

Article

Publisher Correction: N⁶-Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation

In the version of this article originally published, the references were incorrectly re-ordered during production. The hyphen in “N⁶-methyladenosine” in the title was also superscript. The errors have been correc...

Honghui Ma, **aoyun Wang, Jiabin Cai, Qing Dai… in Nature Chemical Biology (2019)

Article

Author Correction: Higher-order epistasis shapes the fitness landscape of a xenobiotic-degrading enzyme

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Gloria Yang, Dave W. Anderson, Florian Baier, Elias Dohmen… in Nature Chemical Biology (2020)

Article

Synthetic yeast brews neuroactive compounds

Metabolic engineering offers the flexibility to meet market demand for bioactive natural products but can be hampered when a necessary protein or intermediate is toxic. In yeast, modifying the subcellular loca...

Yang Gu, Peng Xu in Nature Chemical Biology (2021)

Article

Ligand recognition and G-protein coupling selectivity of cholecystokinin A receptor

Cholecystokinin A receptor (CCK_AR) belongs to family A G-protein-coupled receptors and regulates nutrient homeostasis upon stimulation by cholecystokinin (CCK). It is an attractive drug target for gastrointestina...

Qiufeng Liu, Dehua Yang, Youwen Zhuang, Tristan I. Croll… in Nature Chemical Biology (2021)

Article

Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2

Emerging evidence suggests that host glycans influence severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Here, we reveal that the receptor-binding domain (RBD) of the spike (S) protein on...

Linh Nguyen, Kelli A. McCord, Duong T. Bui, Kim M. Bouwman… in Nature Chemical Biology (2022)