-
Article
Open AccessThe pathogen-encoded signalling receptor Tir exploits host-like intrinsic disorder for infection
The translocated intimin receptor (Tir) is an essential type III secretion system (T3SS) effector of attaching and effacing pathogens contributing to the global foodborne disease burden. Tir acts as a cell-sur...
-
Article
Open AccessEndothelial sensing of AHR ligands regulates intestinal homeostasis
Endothelial cells line the blood and lymphatic vasculature, and act as an essential physical barrier, control nutrient transport, facilitate tissue immunosurveillance and coordinate angiogenesis and lymphangio...
-
Article
Open AccessAuthor Correction: OmpK36-mediated Carbapenem resistance attenuates ST258 Klebsiella pneumoniae in vivo
-
Article
Open AccessMating pair stabilization mediates bacterial conjugation species specificity
Bacterial conjugation mediates contact-dependent transfer of DNA from donor to recipient bacteria, thus facilitating the spread of virulence and resistance plasmids. Here we describe how variants of the plasmi...
-
Article
Open AccessFlagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments
Flagellar filaments function as the propellers of the bacterial flagellum and their supercoiling is key to motility. The outer domains on the surface of the filament are non-critical for motility in many bacte...
-
Protocol
Overview of the Effect of Citrobacter rodentium Infection on Host Metabolism and the Microbiota
Citrobacter rodentium is a natural enteric mouse pathogen that models human intestinal diseases, such as pathogenic E. coli infections, ulcerative colitis, and colon cancer. Upon reaching the monolayer of intesti...
-
Article
Citrobacter rodentium–host–microbiota interactions: immunity, bioenergetics and metabolism
Citrobacter rodentium is an extracellular enteric mouse-specific pathogen used to model infections with human pathogenic Escherichia coli and inflammatory bowel disease. C. rodentium injects type III secretion sy...
-
Article
Open AccessOmpK36-mediated Carbapenem resistance attenuates ST258 Klebsiella pneumoniae in vivo
Carbapenem-resistance in Klebsiella pneumoniae (KP) sequence type ST258 is mediated by carbapenemases (e.g. KPC-2) and loss or modification of the major non-selective porins OmpK35 and OmpK36. However, the mechan...
-
Protocol
The Galleria mellonella Infection Model for Investigating the Molecular Mechanisms of Legionella Virulence
Legionella species evolved virulence factors to exploit protozoa as replicative niches in the environment. Cell culture infection models demonstrated that many of these factors also enable the bacteria to thrive ...
-
Protocol
Determination of In Vivo Interactomes of Dot/Icm Type IV Secretion System Effectors by Tandem Affinity Purification
The Dot/Icm type IV secretion system (T4SS) is essential for the pathogenesis of Legionella species and translocates a multitude of effector proteins into host cells. The identification of host cell targets of th...
-
Article
Open AccessAuthor Correction: Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion
The original version of this Article contained an error in the spelling of the author David Ruano-Gallego, which was incorrectly given as David R. Gallego. This has now been corrected in both the PDF and HTML ...
-
Article
Open AccessHost-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion
Niche-adaptation of a bacterial pathogen hinges on the ability to recognize the complexity of signals from the environment and integrate that information with the regulation of genes critical for infection. He...
-
Article
Sieving through gut models of colonization resistance
The development of innovative high-throughput genomics and metabolomics technologies has considerably expanded our understanding of the commensal microorganisms residing within the human body, collectively ter...
-
Chapter
The Type III Secretion System of Pathogenic Escherichia coli
Infection with enteropathogenic and enterohaemorrhagic Escherichia coli (EPEC and EHEC), enteroinvasive E. coli (EIEC) and Shigella relies on the elaboration of a type III secretion system (T3SS). Few strains als...
-
Article
Erratum: EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation
Nature Microbiology 2, 16258 (2017); published online 13 January 2017; corrected 13 February 2017 In the original HTML version of this Letter, the author information appeared incorrectly. Jaclyn S. Pearson and...
-
Article
EspL is a bacterial cysteine protease effector that cleaves RHIM proteins to block necroptosis and inflammation
Cell death signalling pathways contribute to tissue homeostasis and provide innate protection from infection. Adaptor proteins such as receptor-interacting serine/threonine-protein kinase 1 (RIPK1), receptor-i...
-
Article
Citrobacter rodentium mouse model of bacterial infection
Infection of mice with Citrobacter rodentium is the gold-standard method for studying virulence of the closely related human pathogens enteropathogenic and enterohemorrhagic Escherichia coli. This protocol detail...
-
Article
Open AccessThe Hd, Hj, and Hz66 flagella variants of Salmonella enterica serovar Typhi modify host responses and cellular interactions
Salmonella Typhi, the causative agent of typhoid fever, is a monophyletic, human-restricted bacterium that exhibits limited phenotypic variation. S. Typhi from Indonesia are a notable exception, with circulating ...
-
Article
Open AccessThe Escherichia coli effector EspJ blocks Src kinase activity via amidation and ADP ribosylation
The hallmark of enteropathogenic Escherichia coli (EPEC) infection is the formation of actin-rich pedestal-like structures, which are generated following phosphorylation of the bacterial effector Tir by cellular ...
-
Article
Citrobacter rodentium: infection, inflammation and the microbiota
The mouse pathogen Citrobacter rodentium is a useful model to investigate important human intestinal diseases, including enteropathogenic Escherichia coli (EPE...
