42 Result(s)
within
G. Spangenberg
Life Sciences
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Article
Individual selection, culture and manipulation of higher plant cells
Due to the heterogeneity in morphology, physiological and morphogenetical capabilities of higher plant cells in mass culture, the development of methods for individually culturing defined cells seemed to be us...
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Transgenic rapeseed plants obtained by the microinjection of DNA into microspore-derived embryoids
A novel method in the field of genetic engineering of higher plants is presented: microinjection into multicellular structures which have a high competence for plant regeneration through embryogenesis. Microsp...
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Selection, Experimental Manipulation and Culture of Single Protoplasts
Heterogeneity of plant cell cultures with respect of response to culture conditions is one of the major obstacles in experimental manipulation of higher plant cells or protoplasts. One way to overcome this pro...
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Gene Transfer by Individual Manipulation of Plant Cells
The use of an improved microculture system originally developed for protoplasts of Nicotiana tabacum and Datura innoxia cells (Koop et al.1983a, Koop and Schweiger, 1985a) allows the individual selection of singl...
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Article
Somatic hybridization by microfusion of defined protoplast pairs in Nicotiana: morphological, genetic, and molecular characterization
Somatic hybrid/cybrid plants were obtained by microfusion of defined protoplast pairs from malefertile, streptomycin-resistant Nicotiana tabacum and cytoplasmic male-sterile (cms), streptomycin-sensitive N. tabac...
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Organelle transfer by microfusion of defined protoplast-cytoplast pairs
Defined cybridization was performed by one-to-one electrofusion (microfusion) of preselected protoplast-cytoplast pairs of male-fertile, streptomycin-resistant Nicotiana tabacum and cytoplasmic male-sterile, stre...
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Low density cultures: microdroplets and single cell nurse cultures
The development of low density cell culture systems is mainly based on two assumptions. Firstly, although plant cell (protoplast) cultures are commonly referred to as relatively homogeneous cell populations th...
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CMS system inNicotiana: flower development, patterns of mitochondrial DNA and mitochondrial gene expression
A detailed description of in planta floral ontogeny based on scanning electron microscopy, developmental histology and morphology is presented for three different alloplasmic gene-cytoplasmic male-sterile (CMS)Ni...
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Molecular and cytogenetic characterization of repetitive DNA sequences from Lolium and Festuca: applications in the analysis of Festulolium hybrids
A set of species-specific repetitive DNA sequences was isolated from Lolium multiflorum and Festuca arundinacea. The degree of their species specificity as well as possible homologies among them were determined b...
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Analysis of genetic stability of plants regenerated from suspension cultures and protoplasts of meadow fescue (Festuca pratensis Huds.)
A cytological and molecular analysis was performed to assess the genetic uniformity and true-to-type character of plants regenerated from 20 week-old embryogenic suspension cultures of meadow fescue (Festuca prat...
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Fertile plant regeneration from protoplasts of meadow fescue (Festuca pratensis Huds.)
Suspension cultures from mature embryo-derived compact callus were initiated in seven meadow fescue (Festuca pratensis Huds.) cultivars. Four to six months after initiation, embryogenic suspension cultures with a...
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Somatic Hybridization in Festuca and Lolium
Several species in the genus Festuca are commonly grown perennial forage and turf grasses of the temperate geographic region. Particularly tall fescue (F. arundinacea Schreb.) has a wide range of distribution as ...
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Somatic Hybridization by Microfusion of Protoplasts
Somatic hybridization in plants has classically relied on en masse fusion of protoplasts. In general, large populations of protoplasts isolated from both parental forms are mixed and subjected to fusogenic tre...
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Article
Asymmetric somatic hybridization between tall fescue (Festuca arundinacea Schreb.) and irradiated Italian ryegrass (Lolium multiflorum Lam.) protoplasts
Intergeneric asymmetric somatic hybrids have been obtained by the fusion of metabolically inactivated protoplasts from embryogenic suspension cultures ofFestuca arundinacea (recipient) and protoplasts from a non-...
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Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and their Protoplasts in Forage Grasses
An efficient system for green plant regeneration from embryogenic suspensions and corresponding protoplasts in the temperate forage and turf grass species: Festuca arundinacea, F. pratensis, F. rubra, Lolium mult...
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Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants
The protocol detailed below describes the procedure for the transient and stable transformation of leaf protoplasts obtained from axenic shoot cultures of Nicotiana tabacum L. cv. Petit Havana SR1, a widely used ...
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Plant Regeneration from Suspension and Protoplast Cultures in the Temperate Grasses Festuca and Lolium
An efficient system for green plant regeneration from protoplasts in different Festuca and Lolium species: F. arundinacea, F. pratensis, F. rubra, L. multiflorum, L. perenne and L. × boucheanum has been worked ou...
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Genetic Transformation in Festuca arundinacea Schreb. (Tall Fescue) and Festuca pratensis Huds. (Meadow Fescue)
Tall fescue (Festuca arundinacea Schreb.) is a wind-pollinated, highly self-infertile polyploid perennial cool-season forage, turf, and conservation grass. It is indigenous to Europe, also naturally occuring on t...
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Intergeneric symmetric and asymmetric somatic hybridization in Festuca and Lolium
Intergeneric symmetric and asymmetric somatic hybrids have been obtained by fusion of metabolically inactivated protoplasts from embryogenic suspension cultures of tall fescue (Festuca arundinacea Schreb.) and un...
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GENE Technology for Develo** Countries: Genetic Engineering of Indica Rice
Indica-type rice provides the staple food for 2 billion people in Third World Countries. We have established gene transfer to IRRI breeding lines to explore the contributions of genetic engineering to sustaine...
