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  1. No Access

    Chapter

    Low-pollen-allergen ryegrasses: towards a continent free of hay fever?

    The ryegrasses (Lolium spp.) are the major grass species sown for forage and amenity use in temperate areas of the world. Pollen of ryegrass is a widespread source of airborne allergens and is a major cause of ha...

    G. Spangenberg, N. Petrovska, G. A. Kearney, K. F. Smith in Allergy Matters (2006)

  2. No Access

    Chapter and Conference Paper

    Gene Discovery and Microarray-Based Transcriptome Analysis in Grass Endophytes

    A genomic resource of 9411 expressed sequence tags (ESTs) representing 5038 genes has been established for Neotyphodium and Epichloë endophytes. A cDNA spotted microarray interrogating 4195 Neotyphodium and 920 E...

    S. Felitti, K. Shields, M. Ramsperger, P. Tian… in Molecular Breeding of Forage and Turf (2004)

  3. No Access

    Chapter and Conference Paper

    Field Evaluation of Transgenic White Clover with AMV Immunity and Development of Elite Transgenic Germplasm

    Viral diseases such as alfalfa mosaic virus (AMV) cause significant reductions in dry matter yield and persistency of white clover (Trifolium repens L.). Transgenic white clover plants expressing the AMV coat pro...

    M. Emmerling, P. Chu, K. Smith, R. Kalla… in Molecular Breeding of Forage and Turf (2004)

  4. No Access

    Chapter

    Establishment of and Plant Regeneration from Embryogenic Cell Suspensions and their Protoplasts in Forage Grasses

    An efficient system for green plant regeneration from embryogenic suspensions and corresponding protoplasts in the temperate forage and turf grass species: Festuca arundinacea, F. pratensis, F. rubra, Lolium mult...

    Z. Y. Wang, G. Legris, G. Spangenberg in Gene Transfer to Plants (1995)

  5. No Access

    Chapter

    Polyethylene Glycol-Mediated Direct Gene Transfer to Tobacco Protoplasts and Regeneration of Transgenic Plants

    The protocol detailed below describes the procedure for the transient and stable transformation of leaf protoplasts obtained from axenic shoot cultures of Nicotiana tabacum L. cv. Petit Havana SR1, a widely used ...

    G. Spangenberg, I. Potrykus in Gene Transfer to Plants (1995)

  6. No Access

    Chapter

    Standard Molecular Techniques for the Analysis of Transgenic Plants

    In the following sections, a series of step-by-step protocols for standard molecular techniques widely used in the characterization of transgenic plants and in gene expression studies with plant cells are comp...

    J. Fütterer, A. Gisel, V. Iglesias, A. Klöti, B. Kost… in Gene Transfer to Plants (1995)