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  1. No Access

    Article

    Precise DNA cleavage using CRISPR-SpRYgests

    Methods for in vitro DNA cleavage and molecular cloning remain unable to precisely cleave DNA directly adjacent to bases of interest. Restriction enzymes (REs) must bind specific motifs, whereas wild-type CRIS...

    Kathleen A. Christie, Jimmy A. Guo, Rachel A. Silverstein… in Nature Biotechnology (2023)

  2. Article

    Open Access

    Characterization of Cme and Yme thermostable Cas12a orthologs

    CRISPR-Cas12a proteins are RNA-guided endonucleases that cleave invading DNA containing target sequences adjacent to protospacer adjacent motifs (PAM). Cas12a orthologs have been repurposed for genome editing ...

    Ryan T. Fuchs, Jennifer L. Curcuru, Megumu Mabuchi… in Communications Biology (2022)

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  3. Article

    Open Access

    A catalogue of biochemically diverse CRISPR-Cas9 orthologs

    Bacterial Cas9 nucleases from type II CRISPR-Cas antiviral defence systems have been repurposed as genome editing tools. Although these proteins are found in many microbes, only a handful of variants are used ...

    Giedrius Gasiunas, Joshua K. Young, Tautvydas Karvelis… in Nature Communications (2020)

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  4. No Access

    Article

    Dynamic RNA acetylation revealed by quantitative cross-evolutionary map**

    N4-acetylcytidine (ac4C) is an ancient and highly conserved RNA modification that is present on tRNA and rRNA and has recently been investigated in eukaryotic mRNA1–3. However, the distribution, dynamics and func...

    Aldema Sas-Chen, Justin M. Thomas, Donna Matzov, Masato Taoka, Kellie D. Nance… in Nature (2020)

  5. No Access

    Article

    Target-dependent nickase activities of the CRISPR–Cas nucleases Cpf1 and Cas9

    Clustered regularly interspaced short palindromic repeats (CRISPR) machineries are prokaryotic immune systems that have been adapted as versatile gene editing and manipulation tools. We found that CRISPR nucle...

    Becky Xu Hua Fu, Justin D. Smith, Ryan T. Fuchs, Megumu Mabuchi… in Nature Microbiology (2019)

  6. No Access

    Article

    Specific and potent RNAi in the nucleus of human cells

    RNA interference (RNAi) has become a research tool to control gene expression in various organisms and holds potential as a new therapeutic strategy. The mechanism of small interfering RNA (siRNA)-mediated RNA...

    G Brett Robb, Kirk M Brown, Jaspreet Khurana… in Nature Structural & Molecular Biology (2005)