-
Article
Open AccessCharacterization of the ribonuclease activity on the skin surface
The rapid degradation of ribonucleic acids (RNA) by ubiquitous ribonucleases limits the efficacy of new therapies based on RNA molecules. Therefore, our aim was to characterize the natural ribonuclease activit...
-
Article
Bisucaberin – A dihydroxamate siderophore isolated from Vibrio salmonicida, an important pathogen of farmed Atlantic salmon (Salmo salar)
A siderophore of the bacterial fish pathogen, Vibrio salmonicida, was isolated from low-iron culture supernatant and structurally characterized as bisucaberin by FTICR- and FAB-MS, NMR and GC-MS analysis of the h...
-
Chapter
Solid-Supported Triazenes as Alkylating Polymers Employed for the Versatile Derivatization of Peptides
Recently, we have shown polymer-bound l-aryl-3-alkyl-triazenes to be versatile tools for the smooth esterification of various carboxylic acids [1], including peptides and products of combinatorial synthesis [2...
-
Chapter
Triphosgene as Peptide Coupling Reagent: Highly Efficient Total Synthesis of Cyclosporin O
Cyclosporins are immunosuppressive cyclic peptides from Cylindrocarpon lucidum Booth and Tolypocladium inflatum Gems. Cyclosporin A (CsA, Sandimmune) is a widely used drug for preventing the rejection of transpla...
-
Chapter
Biaryl-Bridged Lipopeptides from Streptomyces sp. TÜ 6075
HPLC-DAD screening for new metabolites [1] from Streptomyces sp. TÜ 6075 revealed two series of compounds composed of five colorless and seven yellow fractions. For structure elucidation, ES-FTICR-MS, chiral GC-M...
-
Chapter
Shedding Light on the Aglycon Formation of Glycopeptide Antibiotics
Glycopeptide antibiotics, with vancomycin as the most prominent representative, have gained considerable interest over recent years. This is due to their function as antibiotics of last resort for infections o...
-
Chapter
Deamidation within a γ-Gliadin-Derived Peptide Enhances Its Recognition by Serum Antibodies of CD Patients
Coeliac disease (CD) or gluten sensitive enteropathy is an autoimmune disease mainly affecting the proximal small intestine [1]. It is caused in susceptible persons by the ingestion of certain cereal proteins, i....
-
Article
An automated prediction of MHC class I-binding peptides based on positional scanning with peptide libraries
Specificities of three mouse major histocompatibility complex (MHC) class I molecules, Kb, Db, and Ld, were analyzed by positional scanning using combinatorial peptide libraries. The result of the analysis was u...
-
Chapter and Conference Paper
Repertoire forecast of MHC class I binding peptides with peptide libraries
Understanding the repertoire of MHC binding peptides is a pivotal step towards looking into the genetic forces that keep the MHC molecules polymorphic and polygenic. We have attempted to forecast the repertoir...
-
Article
Heterobifunctionalized tetraethylene glycol: A spacer for surface attachment of viral peptide epitopes for ELISA and derivatization of membrane modifying peptides
New hydrophilic linkers of the formula Fmoc-NHCH2CH2COO(CH2CH2O)4X (X=COCH2CH2COOH Fmoc-Ats (2), X=CONHCH2COOH Fmoc-Atg (4) and X=CONHCH2CH2COOH Fmoc-Ata (5) have been prepared by heterobifunctional modification ...
-
Article
A study of extracellular matrix-cell adhesion peptidic epitopes related to human serum amyloid A (SAA)
Serum amyloid A (SAA), an acute-phase protein, exists normally in the serum while complexed with high-density lipoprotein 3 (SAA-HDL3). Its levels increase markedly during inflammatory diseases. The pentapeptide ...
-
Article
Heterobifunctionalized tetraethylene glycol: A spacer for surface attachment of viral peptide epitopes for ELISA and derivatization of membrane modifying peptides
New hydrophilic linkers of the formula Fmoc- NHCH2CH2COO(CH2CH2O)4X (X = COCH2CH2COOH Fmoc-Ats (2), X = CONHCH2COOH Fmoc-Atg (4), and X = CONHCH2CH2COOH Fmoc-Ata (5)) have been prepared by heterobifunctional m...
-
Article
A study of extracellular matrix-cell adhesion peptidic epitopes related to human serum amyloid A (SAA)
Serum amyloid A (SAA), an acute-phase protein, exists normally in the serum while complexed with high-density lipoprotein 3 (SAA- HDL3). Its levels increase markedly during inflammatory diseases. The pentapept...
-
Article
Synthesis of peptidomimetics using a polymer-bound Boc-linker
Boc-resin-bound α-hydroxy-β-amino-aldehydes are accessible starting from N-terminally bound amino acidesters by using Dondoni's C1-homologationreaction sequence. The conversion of these synthons totwo different p...
-
Article
Oligocarbamates as MHC class I ligands
New ligands for major histocompatibility complex (MHC) class I molecules were prepared using a flexible automated synthesis of oligocarbamates. An efficient solution-phase synthesis was found for Fmoc-amino al...
-
Article
Oligocarbamates as MHC class I ligands
New ligands for major histocompatibility complex (MHC) class I molecules were prepared using a flexible automated synthesis of oligocarbamates. An efficient solution-phase synthesis was found for Fmoc-amino al...
-
Chapter
A Novel Series of Serum-Resistant Lipoaminoacid Compounds for Cellular Delivery of Plasmid DNA
A variety of non-viral methods have been developed to accomplish gene transfer into eukaryotic cells. Advanced non-viral gene delivery vesicles include poly-lysine conjugates 1–3, polymers 4, dendrimers 5–7, a...
-
Protocol
Map** of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis
This chapter focuses on methods for epitope map** on novel viral polypeptrdes and on fine tuning sensitivity and spectficity of the identified peptide antigens for application in virus diagnosis. Because of ...
-
Article
Solid phase synthesis of allylic alcohols via the Baylis-Hillman reaction
An efficient method for the solid phase synthesis of allylic alcohols via the Baylis-Hillman reaction has been developed. In the presence of DABCO® or 3-quinuclidinol the coupling of resin bound acrylic acid w...
-
Article
Composition and Purity of Combinatorial Aryl Ether Collections Analyzed by Electrospray Mass Spectrometry
Electrospray mass spectrometry (ESI-MS), tandem massspectrometry and on-line RP-HPLC-ESI-MS were used toevaluate the composition and purity of three differentaryl ether mixtures consisting of 10 and 45 aryleth...
