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Open AccessValidation of the predictive value of BDNF -87 methylation for antidepressant treatment success in severely depressed patients—a randomized rater-blinded trial
Brain-derived neurotrophic factor (BDNF) is essential for antidepressant treatment of major depressive disorder (MDD). Our repeated studies suggest that DNA methylation of a specific CpG site in the promoter r...
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Open AccessAuthor Correction: Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors
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Open AccessCorrection: Neural stem cells genetically-modified to express neprilysin reduce pathology in Alzheimer transgenic models
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Article
Open AccessImagery rescripting and cognitive restructuring for inpatients with moderate and severe depression – a controlled pilot study
This controlled pilot study investigates the effect of the combined use of cognitive restructuring (CR) and imagery rescripting (IR) compared to treatment as usual among inpatients with moderate and severe dep...
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Article
Open AccessAutonomous transposons tune their sequences to ensure somatic suppression
Transposable elements (TEs) are a major constituent of human genes, occupying approximately half of the intronic space. During pre-messenger RNA synthesis, intronic TEs are transcribed along with their host ge...
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Article
Open AccessComparison of methylation estimates obtained via MinION nanopore sequencing and sanger bisulfite sequencing in the TRPA1 promoter region
Bisulfite sequencing has long been considered the gold standard for measuring DNA methylation at single CpG resolution. However, in recent years several new approaches like nanopore sequencing have been develo...
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Open AccessDynamic antagonism between key repressive pathways maintains the placental epigenome
DNA and Histone 3 Lysine 27 methylation typically function as repressive modifications and operate within distinct genomic compartments. In mammals, the majority of the genome is kept in a DNA methylated state...
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Open AccessEnhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells
Cerebral organoids exhibit broad regional heterogeneity accompanied by limited cortical cellular diversity despite the tremendous upsurge in derivation methods, suggesting inadequate patterning of early neural...
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Article
Open AccessDnmt1 has de novo activity targeted to transposable elements
DNA methylation plays a critical role during development, particularly in repressing retrotransposons. The mammalian methylation landscape is dependent on the combined activities of the canonical maintenance e...
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Open AccessAn automated and high-throughput-screening compatible pluripotent stem cell-based test platform for developmental and reproductive toxicity assessment of small molecule compounds
The embryonic stem cell test (EST) represents the only validated and accepted in vitro system for the detection and classification of compounds according to their developmental and reproductive teratogenic pot...
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Article
TETs compete with DNMT3 activity in pluripotent cells at thousands of methylated somatic enhancers
Mammalian cells stably maintain high levels of DNA methylation despite expressing both positive (DNMT3A/B) and negative (TET1-3) regulators. Here, we analyzed the independent and combined effects of these regu...
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Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing
Expansions of short tandem repeats are genetic variants that have been implicated in several neuropsychiatric and other disorders, but their assessment remains challenging with current polymerase-based methods1–4
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Open AccessAutomated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors
The culture of human induced pluripotent stem cells (hiPSCs) at large scale becomes feasible with the aid of scalable suspension setups in continuously stirred tank reactors (CSTRs). Innovative monitoring opti...
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Utilizing Regulatory Networks for Pluripotency Assessment in Stem Cells
Pluripotency is a term in cell biology describing a unique state present in distinct stem cell lines, which were either established from the inner cell mass of the mammalian embryo or derived from somatic cell...
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Open AccessPrincipal components analysis and the reported low intrinsic dimensionality of gene expression microarray data
Principal components analysis (PCA) is a common unsupervised method for the analysis of gene expression microarray data, providing information on the overall structure of the analyzed dataset. In the recent ye...
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Article
Clinical and radiological results of patients treated with orthogonal double plating for periprosthetic femoral fractures
The aim of this study was to determine the outcome of surgically-treated periprosthetic femoral fractures with an orthogonal double plate system.
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Article
A compass for stem-cell differentiation
The development of CellNet — network-biology software that determines how cell types generated in vitro relate to their naturally occurring counterparts — could improve our ability to produce desirable cells in c...
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Open AccessNeural stem cells genetically-modified to express neprilysin reduce pathology in Alzheimer transgenic models
Short-term neural stem cell (NSC) transplantation improves cognition in Alzheimer’s disease (AD) transgenic mice by enhancing endogenous synaptic connectivity. However, this approach has no effect on the under...
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Article
The anterior subcutaneous internal fixator (ASIF) for unstable pelvic ring fractures. Clinical and radiological mid-term results
The purpose of this study was to determine the outcome of unstable type C pelvic fractures treated with posterior stabilisation and the anterior subcutaneous internal fixator (ASIF).
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Screw placement in percutaneous acetabular surgery: gender differences of anatomical landmarks in a cadaveric study
Percutaneous reduction and periarticular screw implantation techniques have been successfully introduced in acetabular surgery. The advantages of this less invasive approach are attenuated by higher risks of s...
