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Article
A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector
Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, ind...
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Article
Open AccessIdentification and rapid map** of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA capture technologies
A broad-spectrum late blight disease-resistance gene from Solanum verrucosum has been mapped to potato chromosome 9. The gene is distinct from previously identified-resistance genes.
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Article
Open AccessHigh contiguity Arabidopsis thaliana genome assembly with a single nanopore flow cell
The handheld Oxford Nanopore MinION sequencer generates ultra-long reads with minimal cost and time requirements, which makes sequencing genomes at the bench feasible. Here, we sequence the gold standard Arabidop...
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Article
Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing
A method for rapid cloning of plant disease-resistance genes could provide sustainable genetic solutions to crop pests and pathogens in place of agrichemicals.
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Article
Open AccessHypersensitive response to Potato virus Y in potato cultivar Sárpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX
Potato virus Y (PVY, Potyvirus) is the fifth most important plant virus worldwide in terms of economic and scientific impact. It infects members of the family Solanaceae and causes losses in pota...
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Article
Open AccessDefining the full tomato NB-LRR resistance gene repertoire using genomic and cDNA RenSeq
The availability of draft crop plant genomes allows the prediction of the full complement of genes that encode NB-LRR resistance gene homologs, enabling a more targeted breeding for disease resistance. Recentl...
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Protocol
Genomic DNA Library Preparation for Resistance Gene Enrichment and Sequencing (RenSeq) in Plants
Enrichment of genomic DNA for genes of interest prior to high-throughput sequencing offers an efficient and cost-effective approach to reduce genome complexity. Target enrichment typically yields higher read-d...
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Article
Open AccessIdentification and localisation of the NB-LRR gene family within the potato genome
The potato genome sequence derived from the Solanum tuberosum Group Phureja clone DM1-3 516 R44 provides unparalleled insight into the genome composition and organisation of this important crop. A key class of ge...
