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Article
Multicellular tumor spheroid model to study the multifaceted role of tumor-associated macrophages in PDAC
While considerable efforts have been made to develop new therapies, progress in the treatment of pancreatic cancer has so far fallen short of patients’ expectations. This is due in part to the lack of predicti...
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Article
Defining myeloid-derived suppressor cells
Myeloid-derived suppressor cells (MDSCs) are cells of neutrophil and monocyte lineages with potent immunosuppressive activity. Numerous studies in mice and humans have identified important roles for MDSCs in s...
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Article
Open AccessCell shape sensing licenses dendritic cells for homeostatic migration to lymph nodes
Immune cells experience large cell shape changes during environmental patrolling because of the physical constraints that they encounter while migrating through tissues. These cells can adapt to such deformati...
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Article
Open AccessSopa: a technology-invariant pipeline for analyses of image-based spatial omics
Spatial omics data allow in-depth analysis of tissue architectures, opening new opportunities for biological discovery. In particular, imaging techniques offer single-cell resolutions, providing essential insi...
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Article
Open AccessSpatially-resolved transcriptomics reveal macrophage heterogeneity and prognostic significance in diffuse large B-cell lymphoma
Macrophages are abundant immune cells in the microenvironment of diffuse large B-cell lymphoma (DLBCL). Macrophage estimation by immunohistochemistry shows varying prognostic significance across studies in DLB...
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Article
Open AccessCebp1 and Cebpβ transcriptional axis controls eosinophilopoiesis in zebrafish
Eosinophils are a group of granulocytes well known for their capacity to protect the host from parasites and regulate immune function. Diverse biological roles for eosinophils have been increasingly identified...
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Article
Open AccessContribution of circulating monocytes in maintaining homeostasis of resident macrophages in postnatal and young adult mouse cochlea
The percentage of macrophage subpopulations based on their origins in the adult cochlea remains unclear. This study aimed to elucidate the origins of cochlear macrophages during the onset phase and development...
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Protocol
Spectral Flow Cytometry Analysis of Resident Tissue Macrophages
Spectral flow cytometry improves flow cytometry panels by resolving the full emission spectra of individual fluorophores, allowing greater flexibility to incorporate more fluorochromes when designing multicolo...
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Article
Presence of onco-fetal neighborhoods in hepatocellular carcinoma is associated with relapse and response to immunotherapy
Onco-fetal reprogramming of the tumor ecosystem induces fetal developmental signatures in the tumor microenvironment, leading to immunosuppressive features. Here, we employed single-cell RNA sequencing, spatia...
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Article
An IL-4 signalling axis in bone marrow drives pro-tumorigenic myelopoiesis
Myeloid cells are known to suppress antitumour immunity1. However, the molecular drivers of immunosuppressive myeloid cell states are not well defined. Here we used single-cell RNA sequencing of human and mouse n...
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Article
iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer
Microglia are specialized brain-resident macrophages that arise from primitive macrophages colonizing the embryonic brain1. Microglia contribute to multiple aspects of brain development, but their precise roles i...
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Article
IL-1β+ macrophages fuel pathogenic inflammation in pancreatic cancer
Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with high resistance to therapies1. Inflammatory and immunomodulatory signals co-exist in the pancreatic tumour microenvironment, leading to dysregulate...
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Article
Open AccessBrain macrophage development, diversity and dysregulation in health and disease
Brain macrophages include microglia in the parenchyma, border-associated macrophages in the meningeal-choroid plexus-perivascular space, and monocyte-derived macrophages that infiltrate the brain under various...
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Article
Open AccessRoles of macrophages in tumor development: a spatiotemporal perspective
Macrophages are critical regulators of tissue homeostasis but are also abundant in the tumor microenvironment (TME). In both primary tumors and metastases, such tumor-associated macrophages (TAMs) seem to supp...
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Article
Open AccessSingle-cell analyses implicate ascites in remodeling the ecosystems of primary and metastatic tumors in ovarian cancer
Ovarian cancer (OC) is an aggressive gynecological tumor usually diagnosed with widespread metastases and ascites. Here, we depicted a single-cell landscape of the OC ecosystem with five tumor-relevant sites, ...
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Article
Open AccessLactation-associated macrophages exist in murine mammary tissue and human milk
Macrophages are involved in immune defense, organogenesis and tissue homeostasis. Macrophages contribute to the different phases of mammary gland remodeling during development, pregnancy and involution postlac...
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Article
Dedicated macrophages organize and maintain the enteric nervous system
Correct development and maturation of the enteric nervous system (ENS) is critical for survival1. At birth, the ENS is immature and requires considerable refinement to exert its functions in adulthood2. Here we d...
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Article
Open AccessMaternal dendritic cells influence fetal allograft response following murine in-utero hematopoietic stem cell transplantation
Intrauterine hematopoietic stem cell transplantation (IUT), potentially curative in congenital haematological disease, is often inhibited by deleterious immune responses to donor cells resulting in subtherapeu...
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Article
Reply to ‘Reclassification of plasmacytoid dendritic cells as innate lymphocytes is premature’
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Article
Open AccessMafB-restricted local monocyte proliferation precedes lung interstitial macrophage differentiation
Resident tissue macrophages (RTMs) are differentiated immune cells that populate distinct niches and exert important tissue-supportive functions. RTM maintenance is thought to rely either on differentiation fr...
