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  1. No Access

    Article

    Site-specific integration of genes into hot spots for recombination flanking aadA in Tn21 transposons

    Tn21-related transposons are widespread among bacteria and carry various resistance determinants at preferential sites, hs1 and hs2. In an in vivo integrative recombination assay it was demonstrated that these ho...

    E. J. Nücken, R. B. Henschke, F. R. J. Schmidt in Molecular and General Genetics MGG (1991)

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    Article

    Use of the gef gene in a biological containment system in soil microcosms

    Data on the use of a biological containment system in microcosms are presented. The system is based on a transposon Tn5-cassette containing a fusion of the lac promoter and gef controlled by lacIQ. The suicide fu...

    R. B. Henschke, E. J. Henschke, F. R. J. Schmidt in Biology and Fertility of Soils (1991)

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    Article

    Screening of soil bacteria for plasmids carrying antibiotic resistance

    To evaluate the role of plasmids in soil communities antibiotic-resistant bacteria have been isolated from soil. Among them, 419 l-aminopeptidase positive strains (Gram-negative) and 28 l-aminopeptidase negative ...

    R. B. Henschke, F. R. J. Schmidt in Biology and Fertility of Soils (1990)

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    Chapter

    The role of soil bacteria in risk assessment analysis

    There are many possible applications for agronomically important genetically engineered microorganisms (GEMs) which would benefit plant production. These include treatment of legume seeds, biodegradation of xe...

    F. R. J. Schmidt, J. Rosien, A. Brokamp in Bacterial Genetics in Natural Environments (1990)

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    Chapter

    The use of a Sesbania rostrata microcosm for studying gene transfer among microorganisms

    In the past decades, bacteria have frequently been introduced into the soil for commercial reasons such as pest control, pollution abatement (e.g., degradation of xenobiotics) and frost protection of crops. Fu...

    H. Schneidereit, F. R. J. Schmidt in Bacterial Genetics in Natural Environments (1990)

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    Article

    Survival, distribution, and gene transfer of bacteria in a compact soil microcosm system

    A microcosm has been designed for application in risk assessment. It was used to investigate the vertical distribution and survival of bacteria in a soil column. Plasmid transfer was studied in sterile and non...

    R. B. Henschke, F. R. J. Schmidt in Biology and Fertility of Soils (1989)

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    Article

    Fate and dispersal of recombinant bacteria in a soil microcosm containing the earthworm Lumbricus terrestris

    A model ecosystem was designed containing Lumbricus terrestris in soil. The microcosm is suitable for assessing the dispersal and survival of genetically engineered microorganisms. After the inoculation of geneti...

    R. B. Henschke, E. Nücken, F. R. J. Schmidt in Biology and Fertility of Soils (1989)

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    Chapter and Conference Paper

    Studies on the Fate and Genetic Stability of Recombinant Microorganisms in Model Ecosystems

    Risk assessment studies concering deliberate release of recombinant microorganisms were performed in micro-ecosystems. For this purpose chemostate and microcosm model systems were designed comprising plain soi...

    F. R. J. Schmidt, R. Henschke, E. Nücken in Risk Assessment for Deliberate Releases (1988)