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Article
Site-specific integration of genes into hot spots for recombination flanking aadA in Tn21 transposons
Tn21-related transposons are widespread among bacteria and carry various resistance determinants at preferential sites, hs1 and hs2. In an in vivo integrative recombination assay it was demonstrated that these ho...
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Article
Use of the gef gene in a biological containment system in soil microcosms
Data on the use of a biological containment system in microcosms are presented. The system is based on a transposon Tn5-cassette containing a fusion of the lac promoter and gef controlled by lacIQ. The suicide fu...
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Article
Screening of soil bacteria for plasmids carrying antibiotic resistance
To evaluate the role of plasmids in soil communities antibiotic-resistant bacteria have been isolated from soil. Among them, 419 l-aminopeptidase positive strains (Gram-negative) and 28 l-aminopeptidase negative ...
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Chapter
The role of soil bacteria in risk assessment analysis
There are many possible applications for agronomically important genetically engineered microorganisms (GEMs) which would benefit plant production. These include treatment of legume seeds, biodegradation of xe...
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Chapter
The use of a Sesbania rostrata microcosm for studying gene transfer among microorganisms
In the past decades, bacteria have frequently been introduced into the soil for commercial reasons such as pest control, pollution abatement (e.g., degradation of xenobiotics) and frost protection of crops. Fu...
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Article
Survival, distribution, and gene transfer of bacteria in a compact soil microcosm system
A microcosm has been designed for application in risk assessment. It was used to investigate the vertical distribution and survival of bacteria in a soil column. Plasmid transfer was studied in sterile and non...
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Article
Fate and dispersal of recombinant bacteria in a soil microcosm containing the earthworm Lumbricus terrestris
A model ecosystem was designed containing Lumbricus terrestris in soil. The microcosm is suitable for assessing the dispersal and survival of genetically engineered microorganisms. After the inoculation of geneti...
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Chapter and Conference Paper
Studies on the Fate and Genetic Stability of Recombinant Microorganisms in Model Ecosystems
Risk assessment studies concering deliberate release of recombinant microorganisms were performed in micro-ecosystems. For this purpose chemostate and microcosm model systems were designed comprising plain soi...