12 Result(s)
within
Eun-Ji Kim
Microbiology
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Article
Open AccessHarnessing novel engineered feeder cells expressing activating molecules for optimal expansion of NK cells with potent antitumor activity
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Article
Differences in seroprevalence between epicenter and non-epicenter areas of the COVID-19 outbreak in South Korea
To compare the standardized severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) seroprevalence of high epicenter region with non-epicenter region, serological studies were performed with a total of 3,...
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Article
Algicidal effect of hybrid peptides as potential inhibitors of harmful algal blooms
To biochemically characterize synthetic peptides to control harmful algal blooms (HABs) that cause red tides in marine water ecosystems.
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Article
Characterization and engineering of the ethylmalonyl-CoA pathway towards the improved heterologous production of polyketides in Streptomyces venezuelae
Streptomyces venezuelae has an inherent advantage as a heterologous host for polyketide production due to its fast rate of growth that cannot be endowed easily through metabolic engineeri...
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Article
Achievements and impacts of glycosylation reactions involved in natural product biosynthesis in prokaryotes
Bioactive natural products, such as polyketides, flavonoids, glycopeptides, and aminoglycosides, have been used as therapeutic agents. Many of them contain structurally diverse sugar moieties attached to the a...
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Article
Engineered biosynthesis of glycosylated derivatives of narbomycin and evaluation of their antibacterial activities
A 14-membered macrolide antibiotic narbomycin produced from Streptomyces venezuelae ATCC 15439 is composed of polyketide macrolactone ring and D-desosamine as a deoxysugar moiety, which acts as an important deter...
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Article
A combined approach of classical mutagenesis and rational metabolic engineering improves rapamycin biosynthesis and provides insights into methylmalonyl-CoA precursor supply pathway in Streptomyces hygroscopicus ATCC 29253
Rapamycin is a macrocyclic polyketide with immunosuppressive, antifungal, and anticancer activity produced by Streptomyces hygroscopicus ATCC 29253. Rapamycin production by a mutant strain (UV2-2) induced by ultr...
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Article
Rapid Quantification of Lipids in Acremonium chrysogenum Using Oil Red O
A method based on staining condition and volume of culture broth for the rapid estimation of the level of intracellular lipids in Acremonium chrysogenum using Oil red O was developed. Lipids in A. chrysogenum wer...
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Article
Generation of reduced macrolide analogs by regio-specific biotransformation
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Article
Differentiation of Acremonium chrysogenum M35 in submerged culture with glass beads or silicone rubbers
In this study, we investigated the effects of glass beads and silicone rubbers on the differentiation and morphological changes of A. chrysogenum M35 in submerged culture. Differentiation in the center of the cel...
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Article
Genetic engineering of macrolide biosynthesis: past advances, current state, and future prospects
Polyketides comprise one of the major families of natural products. They are found in a wide variety of bacteria, fungi, and plants and include a large number of medically important compounds. Polyketides are ...
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Article
Heterologous production of epothilones B and D in Streptomyces venezuelae
Epothilones, produced from the myxobacterium Sorangium cellulosum, are potential anticancer agents that stabilize microtubules in a similar manner to paclitaxel. The entire epothilone biosynthetic gene cluster wa...
