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Open AccessTwo-component latency distributions indicate two-step vesicular release at simple glutamatergic synapses
It is often assumed that only stably docked synaptic vesicles can fuse following presynaptic action potential stimulation. However, during action potential trains docking sites are increasingly depleted, raisi...
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Article
Open AccessNeurosecretion: what can we learn from chromaffin cells
Many of the molecular players in the stimulus-secretion chain are similarly active in neurosecretion and catecholamine release. Therefore, studying chromaffin cells uncovered many details of the processes of d...
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Article
Open AccessIon channels: their discovery, their function, and their role in disease
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Protein scaffolds in the coupling of synaptic exocytosis and endocytosis
Nervous system function crucially depends on synaptic vesicle exocytosis and endocytosis at defined areas within the nerve terminal. Exocytosis preferentially ...
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Article
Open AccessModel of synaptic transmission in the calyx of Held
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Open AccessInformation transmission by synapses with short-term synaptic plasticity
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A comparison between exocytic control mechanisms in adrenal chromaffin cells and a glutamatergic synapse
It has been known since the work of Katz and collaborators in the early 1950s that an increase in intracellular Ca++ concentration ([Ca++]) is the immediate trigger for neurotransmitter release [1]. Later work ha...
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Article
Direct modulation of synaptic vesicle priming by GABAB receptor activation at a glutamatergic synapse
Second messenger cascades involving G proteins1,2 and calcium3 are known to modulate neurotransmitter release4,5. A prominent effect of such a cascade is the downmodulation of presynaptic calcium influx6,7, which...
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Molecular biology meets microelectronics
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Article
Intracellular calcium dependence of transmitter release rates at a fast central synapse
Calcium-triggered fusion of synaptic vesicles and neurotransmitter release are fundamental signalling steps in the central nervous system. It is generally assumed that fast transmitter release is triggered by ...
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Plenary lectures
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Article
Multiple kinetic components of exocytosis distinguished by neurotoxin sensitivity
The secretion of synaptic and other vesicles is a complex process involving multiple steps. Many molecular components of the secretory apparatus have been identified, but how they relate to the different stage...
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Help from fast synapses
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Article
Swelling-induced catecholamine secretion recorded from single chromaffin cells
We have studied osmotically induced catecholamine secretion from bovine adrenal chromaffin cells by combining patch-clamp measurements, electrochemical detection of secretion, and Fura-2 measurements of intrac...
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Book
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Chapter
Voltage Offsets in Patch-Clamp Experiments
Offset voltages of various origins have to be considered in patch-clamp experiments. Some of the offsets are constant during a typical experiment, such as amplifier input offsets; some are variable, such as li...
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Chapter
Geometric Parameters of Pipettes and Membrane Patches
The object of the patch-current measurement is a small membrane patch and a pipette tip of comparable dimensions. Both are at the limit of resolution of the optical microscope. Only rarely is it possible to “s...
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Chapter
Tight-Seal Whole-Cell Recording
The tight-seal whole-cell recording method, often abbreviated as “whole-cell recording” (WCR), allows one to record from cells and modify their internal environment by using a patch-clamp pipette. This has bec...
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Article
Mice sans synaptotagmin
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Article
Calcium dependence of the rate of exocytosis in a synaptic terminal
RAPID calcium-dependent exocytosis underlies neurotransmitter release from nerve terminals. Despite the fundamental importance of this process, neither the relationship between presynaptic intra-cellular calci...
