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Article
Reply to ‘Evolutionary placement of Methanonatronarchaeia’
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Article
Homoectoine Protects Against Colitis by Preventing a Claudin Switch in Epithelial Tight Junctions
Inflammatory bowel diseases (IBD) are multifactorial disorders affecting millions of people worldwide with alarmingly increasing incidences every year. Dysfunction of the intestinal epithelial barrier is assoc...
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Article
Discovery of extremely halophilic, methyl-reducing euryarchaea provides insights into the evolutionary origin of methanogenesis
Methanogenic archaea are major players in the global carbon cycle and in the biotechnology of anaerobic digestion. The phylum Euryarchaeota includes diverse groups of methanogens that are interspersed with non...
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Article
Influence of osmotic stress on desiccation and irradiation tolerance of (hyper)-thermophilic microorganisms
This study examined the influence of prior salt adaptation on the survival rate of (hyper)-thermophilic bacteria and archaea after desiccation and UV or ionizing irradiation treatment. Survival rates after des...
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Article
The hydroxyectoine gene cluster of the non-halophilic acidophile Acidiphilium cryptum
Acidiphilium cryptum is an acidophilic, heterotrophic α-Proteobacterium which thrives in acidic, metal-rich environments (e.g. acid mine drainage). Recently, an ectABCDask gene cluster for biosyn...
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Article
Unexpected property of ectoine synthase and its application for synthesis of the engineered compatible solute ADPC
A new cyclic amino acid was detected in a deletion mutant of the moderately halophilic bacterium Halomonas elongata deficient in ectoine synthesis. Using mass spectroscopy (MS) and nuclear magnetic resonance (NMR...
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Article
Open AccessHeterologous ectoine production in Escherichia coli: By-passing the metabolic bottle-neck
Transcription of the ectoine biosynthesis genes ectA, ectB and ectC from Marinococcus halophilus in recombinant Escherichia coli DH5α is probably initiated from three individual σ70/σA-dependent promoter sequence...
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Article
Influence of salts and pH on growth and activity of a novel facultatively alkaliphilic, extremely salt-tolerant, obligately chemolithoautotrophic sufur-oxidizing Gammaproteobacterium Thioalkalibacter halophilus gen. nov., sp. nov. from South-Western Siberian soda lakes
A chemolithoautotrophic sulfur-oxidizing bacterium (SOB) strain ALCO 1 capable of growing at both near-neutral and extremely alkaline pH was isolated from hypersaline soda lakes in S-W Siberia (Altai, Russia)....
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Article
Open AccessNhaD type sodium/proton-antiporter of Halomonas elongata: a salt stress response mechanism in marine habitats?
Sodium/proton-antiporters (Nha) are known to play an important role in pH- and Na+-homeostasis. In microorganisms several types with different capacity, affinity and selectivity for Na+ and Li+ exist. The homeost...
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Article
Thialkalivibrio halophilus sp. nov., a novel obligately chemolithoautotrophic, facultatively alkaliphilic, and extremely salt-tolerant, sulfur-oxidizing bacterium from a hypersaline alkaline lake
A new chemolithoautotrophic, facultatively alkaliphilic, extremely salt-tolerant, sulfur-oxidizing bacterium was isolated from an alkaline hypersaline lake in the Altai Steppe (Siberia, Russia). According to 1...
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Article
Growth kinetics of haloalkaliphilic, sulfur-oxidizing bacterium Thioalkalivibrio versutus strain ALJ 15 in continuous culture
The chemolithoautotrophic, sulfur-oxidizing bacterium Thioalkalivibrio versutus strain ALJ 15, isolated from a soda lake in Kenya, was grown in a continuous culture, with thiosulfate or polysulfide as growth-limi...
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Article
Intracellular ion and organic solute concentrations of the extremely halophilic bacterium Salinibacter ruber
Salinibacter ruber is a red obligatory aerobic chemoorganotrophic extremely halophilic Bacterium, related to the order Cytophagales. It was isolated from saltern crystallizer ponds, and requires at least 150 g l
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Article
Investigation into a stress-inducible promoter region from Marinococcus halophilus using green fluorescent protein
A 480-bp Marinococcus halophilus DNA-fragment upstream of the ectoine genes ectABC was linked to the reporter gene gfp uv in the stress probe plasmid p...
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Article
Extrinsic protein stabilization by the naturally occurring osmolytes β-hydroxyectoine and betaine
Thermodynamic aspects of protein stabilization by two widespread naturally occurring osmolytes, β-hydroxyectoine and betaine, were studied using differential scanning calorimetry (DSC) and bovine ribonuclease...
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Chapter
Production and Application of Natural Stabilizing Compounds from Halotolerant Bacteria
Marine, halotolerant microorganisms have great potential for the production of organic osmolytes (compatible solutes), which typically attract water and stabilize biological structures in vivo and in vitro [Ga...
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Article
Influence of salt concentration on the susceptibility of moderately halophilic bacteria to antimicrobials and its potential use for genetic transfer studies
The influence of salinity on the susceptibility of 13 moderately halophilic collection strains belonging to the genera Chromohalobacter, Deleya, Halomonas, Vibrio, and Volcaniella to 10 common antimicrobials has ...
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Article
Carnitine: a novel compatible solute in Lactobacillus plantarum
The aim of this study was to unravel the identity of compatible solutes accumulated by Lactobacillus plantarum subjected to osmotic stress. Betaine was accumulated simulataneously with a novel compatible solute i...
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Article
Identification of N δ-acetylornithine as a novel osmolyte in some Gram-positive halophilic eubacteria
In the course of a screening programme for novel natural substances acting as compatible solutes, an unusual amino acid derivative was detected in several halophilic Gram-positive eubacteria. The compound was ...
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Article
Biosynthesis and function of trehalose inEctothiorhodospira halochloris
Trehalose-6-phosphate synthase, catalyzing the reaction between UDP-glucose and glucose 6-phosphate and forming trehalose 6-phosphate, was isolated and partially purified (30-fold) from the phototrophic, haloa...
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Article
Enzyme stabilization be ectoine-type compatible solutes: protection against heating, freezing and drying
The aim of this study was to elucidate the protective effect of the new compatible solutes, ectoine and hydroxyectoine, on two sensitive enzymes (lactic dehydrogenase, phosphofructokinase). The solutes tested ...
