23 Result(s)
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Erika L. F. Holzbaur
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Article
Open AccessAn interphase actin wave promotes mitochondrial content mixing and organelle homeostasis
Across the cell cycle, mitochondrial dynamics are regulated by a cycling wave of actin polymerization/depolymerization. In metaphase, this wave induces actin comet tails on mitochondria that propel these organ...
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Protocol
Employing Live-Cell Imaging to Study Motor-Mediated Transport
Microtubule-based transport is a highly regulated process, requiring kinesin and/or dynein motors, a multitude of motor-associated regulatory proteins including activating adaptors and scaffolding proteins, an...
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Protocol
Single-Molecule Studies of Motor Adaptors Using Cell Lysates
Long-range transport of organelles and other cellular cargoes along microtubules is driven by kinesin and dynein motor proteins in complex with cargo-specific adaptors. While some adaptors interact exclusively...
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Article
Selective motor activation in organelle transport along axons
The active transport of organelles and other cargos along the axon is required to maintain neuronal health and function, but we are just beginning to understand the complex regulatory mechanisms involved. The ...
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Protocol
Live Imaging of Autophagosome Biogenesis and Maturation in Primary Neurons
Live-cell imaging of autophagy in primary neurons has revealed a robust and constitutive pathway for nonselective autophagy in the axon. Autophagosome biogenesis occurs in the distal axon; newly formed autopha...
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Article
Open AccessMitochondrial adaptor TRAK2 activates and functionally links opposing kinesin and dynein motors
Mitochondria are transported along microtubules by opposing kinesin and dynein motors. Kinesin-1 and dynein-dynactin are linked to mitochondria by TRAK proteins, but it is unclear how TRAKs coordinate these mo...
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Article
Actin cables and comet tails organize mitochondrial networks in mitosis
Symmetric cell division requires the even partitioning of genetic information and cytoplasmic contents between daughter cells. Whereas the mechanisms coordinating the segregation of the genome are well known, ...
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Article
Open AccessA tunable LIC1-adaptor interaction modulates dynein activity in a cargo-specific manner
Cytoplasmic dynein-1 (dynein) is the motor responsible for most retrograde transport of cargoes along microtubules in eukaryotic cells, including organelles, mRNA and viruses. Cargo selectivity and activation ...
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Article
The ADP/ATP translocase drives mitophagy independent of nucleotide exchange
Mitochondrial homeostasis depends on mitophagy, the programmed degradation of mitochondria. Only a few proteins are known to participate in mitophagy. Here we develop a multidimensional CRISPR–Cas9 genetic scr...
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Protocol
Imaging the Dynamics of Mitophagy in Live Cells
Investigating the precise spatiotemporal dynamics of mitophagy can provide insights into how mitochondrial quality control is regulated in different tissues and organisms. Here, we outline live imaging assays ...
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Article
Open AccessA conserved interaction of the dynein light intermediate chain with dynein-dynactin effectors necessary for processivity
Cytoplasmic dynein is the major minus-end-directed microtubule-based motor in cells. Dynein processivity and cargo selectivity depend on cargo-specific effectors that, while generally unrelated, share the abil...
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Article
The impact of cytoskeletal organization on the local regulation of neuronal transport
The neuronal cytoskeleton is functionally specialized within distinct compartments; local differences in cytoskeletal organization regulate local traffic patte...
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Article
Open AccessDynamic actin cycling through mitochondrial subpopulations locally regulates the fission–fusion balance within mitochondrial networks
Mitochondria form interconnected networks that dynamically remodel in response to cellular needs. Using live-cell imaging, we investigate the role of the actin cytoskeleton in regulating mitochondrial fission ...
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Protocol
Methods for Assessing Nuclear Rotation and Nuclear Positioning in Develo** Skeletal Muscle Cells
Skeletal muscle cells are large syncytia, containing hundreds of nuclei positioned regularly along the length of the fiber. During development, nuclei are actively distributed throughout the myotube by the mic...
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Article
Dynactin functions as both a dynamic tether and brake during dynein-driven motility
Dynactin is an essential cofactor for most cellular functions of the microtubule motor cytoplasmic dynein, but the mechanism by which dynactin activates dynein remains unclear. Here we use single molecule appr...
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Article
Dynein at odd angles?
Cytoplasmic dynein drives vesicular transport from the periphery to the cell body of neurons. Missense mutations in the dynein tail domain cause neurodegenerative disease in mouse models. New data on the effec...
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Chapter
Axonal Transport and Neurodegenerative Disease
Active intracellular transport is required to maintain the extended cellular processes of neurons. Long-distance transport along the axon is mediated by molecular motor proteins moving along the microtubule cy...
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Article
Processive bidirectional motion of dynein–dynactin complexes in vitro
Cytoplasmic dynein is the primary molecular motor responsible for transport of vesicles, organelles, proteins and RNA cargoes from the periphery of the cell towards the nucleus along the microtubule cytoskelet...
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Article
Tangled NUDELs?
NUDEL was first identified as a protein required for nuclear migration in filamentous fungi. Now, a study shows that it is important for normal assembly of neurofilaments in the mammalian nervous system. In utero
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Article
Mutant dynactin in motor neuron disease
Impaired axonal transport in motor neurons has been proposed as a mechanism for neuronal degeneration in motor neuron disease. Here we show linkage of a lower motor neuron disease to a region of 4 Mb at chromo...
