T-Cell Differentiation
Methods and Protocols
Page
%P
-
Article
Open AccessDirect analysis of thymic function in children with Down's syndrome
Down's syndrome (DS) is characterized by several immunological defects, especially regarding T cell compartment. DS is considered the best example of accelerated ageing in humans. Direct observations of the th...
-
Article
Genetic polymorphisms of Fas (CD95) and Fas ligand (CD178) influence the rise in CD4+ T cell count after antiretroviral therapy in drug-naïve HIV-positive patients
Fas and Fas ligand (FasL) are the main genes that control cell death in the immune system. Indeed, they are crucial for the regulation of T lymphocyte homeostasis because they can influence cell proliferation....
-
Article
Multiparametric analysis of cells with different mitochondrial membrane potential during apoptosis by polychromatic flow cytometry
The analysis of changes in mitochondrial membrane potential (MMP) that can occur during apoptosis provides precious information on the mechanisms and pathways of cell death. For many years, the metachromatic f...
-
Protocol
Investigating T Cells by Polychromatic Flow Cytometry
Since its development, flow cytometry gave a relevant contribution to the field of Immunology. Its unique potential to analyse multiple parameters at the single cell level allowed the identification of unknown...
-
Chapter
Lymphocytes Sub-Types and Functions in Centenarians as Models for Successful Ageing
Several cell subsets participate to the immune response, and their close interplay is fundamental for the successful elimination of harmful pathogens. In addition, a tight regulation of the immune response has...
-
Article
A human memory T cell subset with stem cell–like properties
Whether there exists a human memory T cell population with stem cell–like properties of self-renewal and multipotency is under active investigation. Here Gattinoni et al. characterize a subset of human T cells th...
-
Article
Identification, isolation and in vitro expansion of human and nonhuman primate T stem cell memory cells
The T cell compartment is phenotypically and functionally heterogeneous; subsets of naive and memory cells have different functional properties, and also differ with respect to homeostatic potential and the ab...
-
Book
-
Article
Open AccessMolecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018
Over the past decade, the Nomenclature Committee on Cell Death (NCCD) has formulated guidelines for the definition and interpretation of cell death from morphological, biochemical, and functional perspectives....
-
Article
Development, application and computational analysis of high-dimensional fluorescent antibody panels for single-cell flow cytometry
The interrogation of single cells is revolutionizing biology, especially our understanding of the immune system. Flow cytometry is still one of the most versatile and high-throughput approaches for single-cell...
-
Article
Defining ‘T cell exhaustion’
‘T cell exhaustion’ is a broad term that has been used to describe the response of T cells to chronic antigen stimulation, first in the setting of chronic viral infection but more recently in response to tumou...
-
Article
Open AccessGlobal chromatin conformation differences in the Drosophila dosage compensated chromosome X
In Drosophila melanogaster the single male chromosome X undergoes an average twofold transcriptional upregulation for balancing the transcriptional output between sexes. Previous literature hypothesised that a gl...
-
Article
Open AccessImmunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway
The diversity of the naïve T cell repertoire drives the replenishment potential and capacity of memory T cells to respond to immune challenges. Attrition of the immune system is associated with an increased pr...
-
Article
A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by Treg cells
Genetic variations underlying susceptibility to complex autoimmune and allergic diseases are concentrated within noncoding regulatory elements termed enhancers1. The functions of a large majority of disease-assoc...
-
Article
Two subsets of stem-like CD8+ memory T cell progenitors with distinct fate commitments in humans
T cell memory relies on the generation of antigen-specific progenitors with stem-like properties. However, the identity of these progenitors has remained unclear, precluding a full understanding of the differe...
-
Article
A fresh look at the T helper subset dogma
Single-cell-sequencing techniques enable the decades-old T helper subset dogma to be re-examined in an unsupervised manner, bringing nuance to the definition of known subsets while simultaneously identifying i...
-
Article
Open AccessCirculating mucosal-associated invariant T cells identify patients responding to anti-PD-1 therapy
Immune checkpoint inhibitors are used for treating patients with metastatic melanoma. Since the response to treatment is variable, biomarkers are urgently needed to identify patients who may benefit from such ...
-
Article
Clonally expanded EOMES+ Tr1-like cells in primary and metastatic tumors are associated with disease progression
Regulatory T (Treg) cells are a barrier for tumor immunity and a target for immunotherapy. Using single-cell transcriptomics, we found that CD4+ T cells infiltrating primary and metastatic colorectal cancer and n...
-
Article
Aggressive early-stage lung adenocarcinoma is characterized by epithelial cell plasticity with acquirement of stem-like traits and immune evasion phenotype
Lung adenocarcinoma (LUAD) is the main non-small-cell lung cancer diagnosed in ~40–50% of all lung cancer cases. Despite the improvements in early detection and personalized medicine, even a sizable fraction o...
-
Article
Open AccessSingle-cell profiling defines the prognostic benefit of CD39high tissue resident memory CD8+ T cells in luminal-like breast cancer
Luminal-like breast cancer (BC) constitutes the majority of BC subtypes, but, differently from highly aggressive triple negative BC, is poorly infiltrated by the immune system. The quality of the immune infilt...
