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Chapter
Regeneration of plants from Alfalfa (Medicago sativa L.) Protoplasts by Direct Embryogenesis
Alfalfa plants are usually regenerated from protoplasts with an intervening callus stage (Johnson et al. 1981; Arcioni et al. 1982; Atanassov and Brown 1984; Pezzotti et al. 1984). Because considerable somaclo...
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Article
Callus induction and plant regeneration from anther and inflorescence culture of Sorghum
Twenty-five inbred lines, including grain and forage types from the USA and China, two hybrids, one Sorghum almum, and one Parasorghum (S. versicolor) were tested for their response to anther culture. Three nutri...
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Article
Forage quality of genetically diverse alfalfa germplasms at four phenological growth stages
Forage quality of various alfalfa (Medicago sativa L.) cultivars has been determined under different environmental conditions, and numerous trials with alfalfa have documented an inverse relationship between fora...
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Article
Field resistance of perennial glandular-haired Medicago strains and alfalfa cultivars to the alfalfa weevil
Field plots planted in 1986 to four, perennial, glandular-haired Medicago strains and four alfalfa cultivars were evaluated for alfalfa weevil, [Hypera postica (Gyllenhal)], abundance and feeding damage as well a...
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Article
High transmission of paternal plastid DNA in alfalfa plants demonstrated by restriction fragment polymorphic analysis
A high frequency of paternal plastid transmission occurred in progeny from crosses among normal green alfalfa plants. Plastid transmission was analyzed by hybridization of radiolabeled alfalfa plastid DNA (cpD...
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Article
Glandular hair densities in three perennial Medicago species
Because a number of insect pests periodically reduce the yields and/or quality of alfalfa, control measures are often required to prevent or limit losses. Physical characteristics such as glandular hairs have ...
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Article
Restriction endonuclease analysis of mitochondrial DNA from sorghum with fertile and male-sterile cytoplasms
Mitochondrial DNA from four paired (fertile and male-sterile) lines and six isocytoplasmic strains of sorghum (Sorghum bicolor (L.) Moench) were fragmented by endonucleases and their electrophoretic patterns were...
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Article
The effects of kinetin on callus characters in alfalfa (Medicago sativa L.)
Three callus initiation media, B2-k, B2, and 7951, were used to study the effects of kinetin on callus initiation, morphology, histology, and regenerability in alfalfa (Medicago sativa L.). The presence of kineti...
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Article
Genetic control of in vitro regeneration in alfalfa (Medicago sativa L.)
The genetic control of plant regeneration from callus culture was studied in tetraploid alfalfa (Medicago sativa L.). Seven cultivars (total 72 plants) were screened for regenerability. Ladak had the best regener...
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Article
In vitro culture of pods from annual and perennial Medicago species
Because most interspecific Medicago embryos abort before they can be excised and cultured, our objective was to grow young pods in vitro. Various media were used to grow three-day-old pods of annuals [diploids, M...
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Article
Pollen germination and pollen tube growth following self-pollination and intra- and interspecific pollination of Medicago species
Pollen germination and pollen tube growth after self- and intra- and interspecific pollinations of annual [M. scutellata (L.) Mill., M. disciformis DC., and M. rigidula (L.) All.] and perennial (M. sativa L.) Med...
